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Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

R&D Systems | Catalog # 7754-BH

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein (7754-BH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P01137

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TGF-beta 1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TGF-beta 1 protein
Ala279-Ser390

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala279

Predicted Molecular Mass

12.8 kDa (monomer)

SDS-PAGE

11 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is 0.04-0.2 ng/mL.

Reviewed Applications

Read 16 reviews rated 4.8 using 7754-BH in the following applications:

Scientific Data Images for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Graph showing dose-dependent bioactivity of Human TGF-beta 1 protein

Bioactivity of Human TGF-beta 1 Protein

Recombinant human TGF-beta 1 (7754-BH) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7754-BH
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7754-BH/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 1

TGF‑ beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, ‑2 and ‑3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide-linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta  RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).

References

  1. Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press p. 29.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1985) Nature 316:701.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Gene Symbol

TGFB1

UniProt

P01137 (Human)

Additional TGF-beta 1 Products

Product Documents for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 7754-BH (with carrier)
Download SDS 7754-BH/CF (carrier free)

Product Specific Notices for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

For research use only

Citations for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

Customer Reviews for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein (16)

4.8 out of 5
16 Customer Ratings
5 Stars
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4 Stars
19%
3 Stars
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1 Stars
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Showing  1 - 5 of 16 reviews Showing All
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  • Stimulates human lung samples
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Species: Human
    Verified Customer | Posted 05/04/2026
    Stimulated lung slices with TGFB.
    This was used in a cocktail to stimulate fibrosis and inflammation in human lung slices.
    Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 09/07/2023
    We used several fibroblasts, to assess the immune cells interaction.
    Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Apoptosis assay
    Verified Customer | Posted 02/02/2023
    Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 01/20/2023
    Human liver cells were serum starved over night before TGF treatment.
    Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 11/18/2020
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Yuqing Feng
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/17/2019
  • Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/30/2019
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/07/2019
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 02/10/2019
    TGFB1 human peritoneal mesothelial cells
    Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/22/2018
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 04/12/2018
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 03/08/2018
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 03/07/2018
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Apoptosis assay
    Verified Customer | Posted 02/17/2018
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 02/14/2018
  • Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 10/01/2017
    Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein 7754-BH

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FAQs for Recombinant Human TGF-beta 1 (Human Cell-expressed) Protein

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  • Q: Can Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) be reconstituted in PBS or water?

    A: Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) needs to be reconstituted to 100 μg/mL in sterile 4 mM HCl containing 0.1% BSA or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.

  • Q: Has the half-life of recombinant human TGF beta 1 been determined?

    A: While our Recombinant Human TGF-beta 1 Protein has been evaluated for bioactivity, the half-life of a specific protein will depend on experimental conditions, including the cell number, density, and media content. It is up to the end-user to determine the appropriate concentration and timing when adding Recombinant Human TGF-beta 1 Protein to individual experiments. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

  • Q: Can Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) be reconstituted in PBS or water?

    A: Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) needs to be reconstituted to 100 μg/mL in sterile 4 mM HCl containing 0.1% BSA or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.

  • Q: Has the half-life of recombinant human TGF beta 1 been determined?

    A: While our Recombinant Human TGF-beta 1 Protein has been evaluated for bioactivity, the half-life of a specific protein will depend on experimental conditions, including the cell number, density, and media content. It is up to the end-user to determine the appropriate concentration and timing when adding Recombinant Human TGF-beta 1 Protein to individual experiments. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

  • Q: Can Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) be reconstituted in PBS or water?

    A: Recombinant Human TGF beta 1 Protein (Catalog # 7754-BH or 7754-BH/CF) needs to be reconstituted to 100 μg/mL in sterile 4 mM HCl containing 0.1% BSA or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.

  • Q: Has the half-life of recombinant human TGF beta 1 been determined?

    A: While our Recombinant Human TGF-beta 1 Protein has been evaluated for bioactivity, the half-life of a specific protein will depend on experimental conditions, including the cell number, density, and media content. It is up to the end-user to determine the appropriate concentration and timing when adding Recombinant Human TGF-beta 1 Protein to individual experiments. For techniques and methodologies, we recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

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