Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI9319-050
Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein, CF Binding Activity
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Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human TIM-1/KIM-1/HAVCR Affinity Purified Polyclonal Antibody (Catalog # AF1750) is immobilized at 0.2 μg/mL, 100 μl/well, Recombinant Human TIM-1/KIM-1/HAVCR Fc Chimera Avi-tag Protein (Catalog # AVI9319) binds with an ED50 of 1.5-9 ng/mL. Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.12-1.2 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human TIM-1/KIM-1/HAVCR protein
Human TIM-1/KIM-1/HAVCR
(Ser21-Thr288)
Accession # AAC39862.1
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ser21
Structure / Form
Disulfide-linked homodimer, biotinylated via Avi-tag
Predicted Molecular Mass
57 kDa
SDS-PAGE
115-135 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9319

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity View Larger

When Human TIM-1/KIM-1/HAVCR Affinity Purified Polyclonal Antibody (AF1750) is immobilized at 0.2 μg/mL, 100 μl/well, Recombinant Human TIM-1/KIM-1/HAVCR Fc Avi-tag Protein (AVI9319) binds with an ED50 of 1.5-9 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human TIM-1/KIM-1/HAVCR Fc Chimera Avi-tag (Catalog # AVI9319) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa and 230-270 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIM-1/KIM-1/HAVCR

T cell immunoglobulin and mucin domain 1 (TIM-1), also known as KIM-1 and HAVcr1, is a member of the TIM family which is involved in the regulation of innate and adaptive immune responses (1). TIM-1 is a type I transmembrane protein that contains an N-terminal immunoglobulin-like domain, a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain (2). Multiple TIM-1 variants can be produced due to polymorphisms or alternative splicing resulting in deletions in the mucin domain. Within the extracellular domain, human TIM-1 shares 41% amino acid sequence identity with mouse and rat TIM-1. TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, invariant NKT (iNKT) cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (1, 3-5). It is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (6-9). Metalloproteinase-mediated cleavage of TIM-1 at the membrane-proximal region results in the release of a soluble form of TIM-1 which is detectable in the urine and in circulation (10). TIM-1 serves as a receptor for phosphatidylserine, LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses (2, 11-16). Its interaction with phosphatidylserine enables TIM-1 to mediate the phagocytosis of apoptotic cells (12, 13) and iNKT cell activation (17). TIM-1 binding induces the activation of LMIR5-expressing myeloid cells, contributing to tissue homeostasis as well as damage following kidney injury (14). TIM-1 ligation co-stimulates T cell activation and enhances Th2 cytokine production (7, 15). In humans, TIM-1 serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus and Marburgvirus (2, 11). Our Avi-tag Biotinylated human TIM-1 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Du, P. et al. (2016) J. Immunol. Res. 2016:8605134.
  2. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  3. Ding, Q. et al. (2011) J. Clin. Invest. 121:3645.
  4. Ma, J. et al. (2011) Biochem. Biophys. Res. Commun. 406:223.
  5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
  6. Kuehn, E.W. et al. (2002) Am. J. Physiol. Renal Physiol. 283:F1326.
  7. Umetsu, S.E. et al. (2005) Nat. Immunol. 6:447.
  8. Xiao, S. et al. (2011) Eur. J. Immunol. 41:1539.
  9. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
  10. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  11. Kondratowicz, A.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:8426.
  12. Miyanishi, M. et al. (2007) Nature 450:435.
  13. Kobayashi, N. et al. (2007) Immunity 27:927.
  14. Yamanishi, Y. et al. (2010) J. Exp. Med. 207:1501.
  15. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  16. Tami, C. et al. (2007) J. Virol. 81:3437.
  17. Lee, H.H. et al. (2010) J. Immunol. 185:5225.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

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