Human TIM-1/KIM-1/HAVCR Antibody

Catalog # Availability Size / Price Qty
AF1750
AF1750-SP
Human TIM-1/KIM-1/HAVCR Antibody in Immunohistochemistry
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Product Details
Citations (7)
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Human TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIM‑1/KIM-1/HAVCR in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) TIM-1, recombinant rat TIM-1, and recombinant human (rh) TIM-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM‑1/KIM-1/HAVCR (R&D Systems, Catalog # 1750-TM)
Ser21-Thr288
Accession # Q96D42
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TIM‑1/KIM‑1/HAVCR (Catalog # 1750-TM)
Immunohistochemistry
1-15 µg/mL
See below

Human TIM-1/KIM-1/HAVCR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human TIM‑1/KIM‑1/HAVCR Biotinylated Antibody (Catalog # BAF1750)

Standard: Recombinant Human TIM-1/KIM-1/HAVCR His-tag Protein, CF (Catalog # 1750-TM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry TIM‑1/KIM‑1/HAVCR in Human Kidney Cancer Tissue. View Larger

TIM‑1/KIM‑1/HAVCR in Human Kidney Cancer Tissue. TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 and HAVCR) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).

References
  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  4. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  5. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
  6. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  7. Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43.
  8. Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982.
  9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

Product Datasheets

Citations for Human TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Dynamic Dystroglycan Complexes Mediate Cell Entry of Lassa Virus
    Authors: A Herrador, C Fedeli, E Radulovic, KP Campbell, H Moreno, G Gerold, S Kunz
    MBio, 2019;10(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Human Sertoli cells support high levels of Zika virus replication and persistence
    Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
    Sci Rep, 2018;8(1):5477.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection
    Authors: J Niu, Y Jiang, H Xu, C Zhao, G Zhou, P Chen, R Cao
    Viruses, 2018;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells
    Nat Protoc, 2017;12(1):195-207.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Urinary kidney injury molecule?1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method
    Authors: Y Jin, X Shao, B Sun, C Miao, Z Li, Y Shi
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Urine
    Applications: Immunochromatography
  6. Nanoparticle Detection of Urinary Markers for Point-of-Care Diagnosis of Kidney Injury.
    Authors: Chung H, Pellegrini K, Chung J, Wanigasuriya K, Jayawardene I, Lee K, Lee H, Vaidya V, Weissleder R
    PLoS ONE, 2015;10(7):e0133417.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Development
  7. Characterizing functional domains for TIM-mediated enveloped virus entry.
    Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
    J Virol, 2014;88(12):6702-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human TIM-1/KIM-1/HAVCR Antibody
By Anonymous on 12/15/2017
Application: Detection Assay Sample Tested: Recombinant protein Species: Human