Human TIM-1/KIM-1/HAVCR Antibody
Human TIM-1/KIM-1/HAVCR Antibody Summary
Ser21-Thr288
Accession # Q96D42
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Applications
Human TIM-1/KIM-1/HAVCR Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TIM‑1/KIM‑1/HAVCR in Human Kidney Cancer Tissue. TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TIM-1/KIM-1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIM-1/KIM-1/HAVCR
TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 and HAVCR) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
- Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
- Feigelstock, D. et al. (1998) J. Virol. 72:6621.
- Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
- Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
- Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43.
- Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Product Datasheets
Product Specific Notices
This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued.Citations for Human TIM-1/KIM-1/HAVCR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus
Authors: X Zhang, C Liang, H Wang, Z Guo, H Rong, J Pan, W Li, R Pei, X Chen, Z Zhang, XE Zhang, Z Cui
MBio, 2022;0(0):e0286021.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Single Nucleotide Variants of the Human TIM-1 IgV Domain with Reduced Ability to Promote Viral Entry into Cells
Authors: T Hattori, T Saito, H Miyamoto, M Kajihara, M Igarashi, A Takada
Viruses, 2022;14(10):.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery
Authors: K Hiratsuka, T Miyoshi, KT Kroll, NR Gupta, MT Valerius, T Ferrante, M Yamashita, JA Lewis, R Morizane
Science Advances, 2022;8(38):eabq0866.
Species: Human
Sample Types: Organoids
Applications: IHC -
The Phosphatidylserine Receptor TIM-1 Enhances Authentic Chikungunya Virus Cell Entry
Authors: J Kirui, Y Abidine, A Lenman, K Islam, YD Gwon, L Lasswitz, M Evander, M Bally, G Gerold
Cells, 2021;10(7):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Immortalized stem cell-derived hepatocyte-like cells: An alternative model for studying dengue pathogenesis and therapy
Authors: K Kongmanas, N Punyadee, K Wasuworawo, A Songjaeng, T Prommool, Y Pewkliang, S Manocheewa, S Thiemmeca, K Sa-Ngiamsu, C Puttikhunt, KF Faull, S Hongeng, P Avirutnan
PLoS Negl Trop Dis, 2020;14(11):e0008835.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling
Authors: K Uchimura, H Wu, Y Yoshimura, BD Humphreys
Cell Reports, 2020;33(11):108514.
Species: Human
Sample Types: Organoid
Applications: IHC -
Dynamic Dystroglycan Complexes Mediate Cell Entry of Lassa Virus
Authors: A Herrador, C Fedeli, E Radulovic, KP Campbell, H Moreno, G Gerold, S Kunz
MBio, 2019;10(2):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human Sertoli cells support high levels of Zika virus replication and persistence
Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
Sci Rep, 2018;8(1):5477.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection
Authors: J Niu, Y Jiang, H Xu, C Zhao, G Zhou, P Chen, R Cao
Viruses, 2018;10(11):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells
Nat Protoc, 2017;12(1):195-207.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Urinary kidney injury molecule?1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method
Authors: Y Jin, X Shao, B Sun, C Miao, Z Li, Y Shi
Mol Med Rep, 2017;0(0):.
Species: Rat
Sample Types: Urine
Applications: Immunochromatography -
Nanoparticle Detection of Urinary Markers for Point-of-Care Diagnosis of Kidney Injury.
Authors: Chung H, Pellegrini K, Chung J, Wanigasuriya K, Jayawardene I, Lee K, Lee H, Vaidya V, Weissleder R
PLoS ONE, 2015;10(7):e0133417.
Species: Human
Sample Types: Urine
Applications: ELISA Development -
Characterizing functional domains for TIM-mediated enveloped virus entry.
Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
J Virol, 2014;88(12):6702-13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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