Recombinant Human TLR2 Protein, CF

Catalog # Availability Size / Price Qty
2616-TR-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (13)
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Recombinant Human TLR2 Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity

Measured by its ability to bind biotinylated Pam3CysSerLys4 (PAM3CSK4) (2 μg/mL). The 50% maximum binding is achieved at 0.1‑0.5 μg/mL of Recombinant Human TLR2.

Results can vary with different preparations of biotinylated PAM3CSK4.
Optimal dilutions should be determined by each laboratory for each application.

Source
Mouse myeloma cell line, NS0-derived human TLR2 protein
Glu21-Leu590, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Glu21
Predicted Molecular Mass
65.7 kDa
SDS-PAGE
75-80 kDa, reducing conditions

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2616-TR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2616-TR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG 3350 with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: TLR2

Human toll-like receptor (TLR) family includes ten members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens (1-3). All TLR family members are type I transmembrane proteins with a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic Toll/IL-1 receptor (TIR) domain. Human TLR2 is synthesized as a 784 amino acid precursor (2) that contains a signal sequence (aa 1-18), an extracellular domain (aa 19‑588) with approximately 20 LRRs, a transmembrane segment (aa 589-609), and a cytoplasmic TIR domain (aa 610-784). The receptor is expressed on a number of cell types including monocytes, dendritic cells, neutrophils, B cells endothelial cells, and hepatocytes (1, 2, 4). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors (1). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers bind triacylated lipopeptides, while the TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (5). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 6). TLR2 signaling results in dendritic cell maturation characterized by increased surface expression of class II MHC and the T cell co-stimulators, CD80 and CD86 (1, 2). Activation via TLR2 also results in production of a number of pro‑inflammatory cytokines including TNF-alpha, IL-2, IL-6, IL-12, and MIP-2 (1-3).

References
  1. Wetzler, L. (2003) Vaccine 21:S2/55.
  2. Kirschning, C. and R. Schumann (2002) Curr. Top. microbiol. Immunol. 270:121.
  3. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  4. Flo T. et al. (2001) J. Leukoc. Biol. 69:474.
  5. Akira S. (2003) Curr. Opin. Immunol. 15:5.
  6. Yamamoto M. et al. (2002) Nature 420:324.
Long Name
Toll-like Receptor 2
Entrez Gene IDs
7097 (Human); 24088 (Mouse); 310553 (Rat)
Alternate Names
CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2

Citations for Recombinant Human TLR2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. The GAR/RGG motif defines a family of nuclear alarmins
    Authors: S Wu, BHD Teo, SYK Wee, J Chen, J Lu
    Cell Death & Disease, 2021;12(5):477.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. LYSMD3: A mammalian pattern recognition receptor for chitin
    Authors: X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence
    Cell Reports, 2021;36(3):109392.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis
    Authors: X Xie, H Lv, C Liu, X Su, Z Yu, S Song, H Bian, M Tian, C Qin, J Qi, Q Zhu
    Bmc Medicine, 2021;19(1):247.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot Control
  4. Role of TLR4 Receptor Complex in the Regulation of the Innate Immune Response by Fibronectin
    Authors: M Zheng, A Ambesi, P J McKeown-
    Cells, 2020;9(1):.
    Species: Human
    Sample Types:
    Applications: Cell Culture
  5. Toll-like Receptor 2 Antagonists Identified through Virtual Screening and Experimental Validation
    Authors: P Durai, HJ Shin, A Achek, HK Kwon, RG Govindaraj, S Panneersel, D Yesudhas, J Choi, KT No, S Choi
    FEBS J., 2017;0(0):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  6. Role of CD44 in Regulating TLR2 Activation of Human Macrophages and Downstream Expression of Proinflammatory Cytokines
    Authors: M Qadri, S Almadani, GD Jay, KA Elsaid
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. TLR-2 Recognizes Propionibacterium acnes CAMP Factor 1 from Highly Inflammatory Strains
    PLoS ONE, 2016;11(11):e0167237.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  8. Metalloproteinase-dependent TLR2 ectodomain shedding is involved in soluble toll-like receptor 2 (sTLR2) production.
    Authors: Langjahr P, Diaz-Jimenez D, De la Fuente M, Rubio E, Golenbock D, Bronfman F, Quera R, Gonzalez M, Hermoso M
    PLoS ONE, 2014;9(12):e104624.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding.
    Authors: Sakaguchi M, Murata H, Yamamoto K, Ono T, Sakaguchi Y, Motoyama A, Hibino T, Kataoka K, Huh NH
    PLoS ONE, 2011;6(8):e23132.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Non-acylated Mycobacterium bovis glycoprotein MPB83 binds to TLR1/2 and stimulates production of matrix metalloproteinase 9.
    Authors: Chambers MA, Whelan AO, Spallek R
    Biochem. Biophys. Res. Commun., 2010;400(3):403-8.
    Species: Bacteria
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  11. Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
    Authors: Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
    Clin. Exp. Immunol., 2009;156(2):285-93.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  12. Targeting Toll-like receptors with soluble Toll-like receptor 2 prevents peritoneal dialysis solution-induced fibrosis.
    Authors: Raby A, Gonzalez-Mateo G, Williams A, Topley N, Fraser D, Lopez-Cabrera M, Labeta M
    Kidney Int, 0;94(2):346-362.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Toll-Like Receptors 2 and 4 Are Potential Therapeutic Targets in Peritoneal Dialysis-Associated Fibrosis.
    Authors: Raby A, Colmont C, Kift-Morgan A, Kohl J, Eberl M, Fraser D, Topley N, Labeta M
    J Am Soc Nephrol, 0;28(2):461-478.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human TLR2 Protein, CF
By Anonymous on 08/28/2020
Application: Binding assay/Protein-protein interaction

Different concentrations of human TLR2-His was used to determine the affinity an a TLR2-specific mAb using the Octet system.