Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein, CF

Catalog # Availability Size / Price Qty
11107-TF-050
Recombinant Human TMEFF2/Tomoregulin‑2 His-tag Protein Binding Activity.
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Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human PDGF-AA  (Catalog # 221-AA) is immobilized at 5.00 μg/mL (100 μL/well), Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein binds with an ED50 of 0.750-4.50 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human TMEFF2/Tomoregulin-2 protein
Arg34-Val320, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Arg34
Predicted Molecular Mass
33 kDa
SDS-PAGE
55-66 kDa, under reducing conditions.

Product Datasheets

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11107-TF

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11107-TF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human PDGF-AA (221-AA) is immobilized at 5.00 μg/mL (100 μL/well), Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein (Catalog # 11107-TF) binds with an ED50 of 0.750‑4.50 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human TMEFF2/Tomoregulin‑2 His-tag Protein (Catalog # 11107-TF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-66 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMEFF2/Tomoregulin-2

TMEFF2 (transmembrane protein with an EGF-like and two Follistatin-like domains 2), also known as Tomoregulin-2, is a type I transmembrane glycoprotein that contains two extracellular follistatin modules, an extracellular EGF-like domain, a transmembrane domain, and a short conserved cytoplasmic tail (1) Soluble extracellular domains of both TMEFF1 and 2 can be generated by proteolytic cleavage. TMEFF2 amino acid (aa) sequences are 98.9% identical for human and mouse orthologs (1). The differential methylation of TMEFF2 can be related to the response to therapy and survival outcomes of the stages of cancers such as breast, prostate, lung, bladder, colon and rectal, gallbladder, renal, oesophageal, cardiac, stomach/gastric, ovarian, multiple myeloma, glioblastoma, and mesothelioma. The range of tissue expression of TMEFF2 functions as a possible biomarker and/or therapeutic target (1). Studies showed that TMEFF2 binds and inhibits PDGF-AA (2, 3). It also inhibits the proliferation of DU145 and PC3 prostate cancer cell lines (4). The amyloid-b protein, its precursor AbPP, and amyloid- b proteins AbPOs also bind TMEFF2, suggesting a neuroprotective role on Alzheimer's disease (5). TMEFF2's diverse function includes metabolism, embryonic development, cytoskeletal binding, extracellular matrix binding, chromatin binding and interaction of RNA polymerase II with DNA (6), and neuronal development (7).

References
  1. Masood, M. et al. (2020) Cancers 12:3862.
  2. Lin, K. et al. (2011) PLoS ONE 6:e18608.
  3. Huang, H. et al. (2017) Sci. Rep. 7:337.
  4. Gery, S. et al. (2002) Oncogene 21:4739.
  5. Hong, H.S. et al. (2015) Alzheimers Dis. 48:731.
  6. Gao, L. et al. (2020) Life Sci. 243:116910.
  7. Horie, M. et al. (2000) Genomics 67:146.
Long Name
Transmembrane Protein with EFG-like and two Follistatin-like Domains
Entrez Gene IDs
23671 (Human); 56363 (Mouse)
Alternate Names
CT120.2; HPP1member 2; Hyperplastic polyposis protein 1; TENB2; TMEFF2; Tomoregulin2; Tomoregulin-2; TPEFtomoregulin; TR-2; transmembrane protein TENB2; transmembrane protein with EGF-like and two follistatin-like domains 2; Transmembrane protein with EGF-like and two follistatin-like domains; TRtomoregulin-2

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