Recombinant Human PDGF-AA Protein, CF

Catalog # Availability Size / Price Qty
221-AA-010
221-AA-050
221-AA-025
221-AA-100
221-AA-01M
Graph showing bioactivity of PDGF-AA ability to induce NR6R-3T3 mouse fibroblast cell proliferation
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Product Details
Citations (38)
FAQs
Supplemental Products
Reviews (4)

Recombinant Human PDGF-AA Protein, CF Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002).
Source
E. coli-derived human PDGF-AA protein
Ser87-Thr211, with and without an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Ser87 and Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
29 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

221-AA

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *1 mg pack size (01M) is shipped with dry ice or equivalent. Upon receipt, store it immediately at -20 to -70 °C.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity of Recombinant Human PDGF-AA protein View Larger

Recombinant Human PDGF-AA 221-AA stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 50-200 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-AA

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name
Platelet-derived Growth Factor AA
Entrez Gene IDs
5154 (Human); 25266 (Rat)
Alternate Names
PDGFAA; PDGF-AA

Citations for Recombinant Human PDGF-AA Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Establishment of a simple one-step method for oligodendrocyte progenitor cell preparation from rodent brains
    Authors: A Yoshida, K Takashima, T Shimonaga, M Kadokura, S Nagase, S Koda
    J. Neurosci. Methods, 2020;0(0):108798.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures
    Authors: RM Marton, Y Miura, SA Sloan, Q Li, O Revah, RJ Levy, JR Huguenard, SP Pa?ca
    Nat. Neurosci., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Selective estrogen receptor modulators enhance CNS remyelination independent of estrogen receptors
    Authors: KA Rankin, F Mei, K Kim, YA Shen, SR Mayoral, C Desponts, DS Lorrain, AJ Green, SE Baranzini, JR Chan, R Bove
    J. Neurosci., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair
    Authors: T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton
    Cell Rep, 2019;26(10):2566-2579.e10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The effects of Centella asiatica (L.) Urban on neural differentiation of human mesenchymal stem cells in vitro
    Authors: N Omar, Y Lokanathan, ZR Mohd Razi, R Bt Haji Id
    BMC Complement Altern Med, 2019;19(1):167.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors
    Authors: M Matjusaiti, LJ Wagstaff, A Martella, B Baranowski, C Blin, S Gogolok, A Williams, SM Pollard
    Stem Cell Reports, 2019;13(6):1053-1067.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Teriflunomide promotes oligodendroglial differentiation and myelination
    Authors: P Göttle, A Manousi, D Kremer, L Reiche, HP Hartung, P Küry
    J Neuroinflammation, 2018;15(1):76.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation
    Authors: M Osipovitch, A Asenjo Mar, JN Mariani, A Cornwell, S Dhaliwal, L Zou, D Chandler-M, S Wang, X Li, SJ Benraiss, R Agate, A Lampp, A Benraiss, MS Windrem, SA Goldman
    Cell Stem Cell, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. STAT3 exacerbates survival of cancer stem-like tumorspheres in EGFR-positive colorectal cancers: RNAseq analysis and therapeutic screening
    Authors: CC Cheng, PN Liao, AS Ho, KH Lim, J Chang, YW Su, CG Chen, YW Chiang, BL Yang, HC Lin, YC Chang, CC Chang, YF Chang
    J. Biomed. Sci., 2018;25(1):60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Nintedanib decreases muscle fibrosis and improves muscle function in a murine model of dystrophinopathy
    Authors: P Piñol-Jura, X Suárez-Cal, E Fernández-, E Gallardo, N de la Oliv, A Martínez-M, P Gómez-Gálv, LM Escudero, M Pérez-Peir, L Wollin, N de Luna, X Navarro, I Illa, J Díaz-Maner
    Cell Death Dis, 2018;9(7):776.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3
    Authors: H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng
    Sci Rep, 2017;7(0):43736.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells
    Authors: T Yamashita, Y Miyamoto, Y Bando, T Ono, S Kobayashi, A Doi, T Araki, Y Kato, T Shirakawa, Y Suzuki, J Yamauchi, S Yoshida, N Sato
    PLoS ONE, 2017;12(2):e0171947.
    Species: Primate
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Expression of the Human Herpesvirus 6A Latency-Associated Transcript U94A Disrupts Human Oligodendrocyte Progenitor Migration
    Authors: A Campbell, JM Hogestyn, CJ Folts, B Lopez, C Pröschel, D Mock, M Mayer-Prös
    Sci Rep, 2017;7(1):3978.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Store-operated calcium entry is essential for glial calcium signalling in CNS white matter
    Authors: M Papanikola, A Lewis, AM Butt
    Brain Struct Funct, 2017;0(0):.
    Species: Mouse
    Sample Types: Optic Nerve Explant
    Applications: Bioassay
  15. Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics
    Authors: SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede
    Sci Rep, 2017;7(1):16439.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  16. Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry
    Authors: Y Wu, A Prager, S Boos, M Resch, I Brizic, M Mach, S Wildner, L Scrivano, B Adler
    PLoS Pathog., 2017;13(4):e1006281.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells
    Authors: R Santos, KC Vadodaria, BN Jaeger, A Mei, S Lefcochilo, APD Mendes, G Erikson, M Shokhirev, L Randolph-M, C Fredlender, S Dave, R Oefner, C Fitzpatric, M Pena, JJ Barron, M Ku, AM Denli, BE Kerman, P Charnay, JR Kelsoe, MC Marchetto, FH Gage
    Stem Cell Reports, 2017;8(6):1757-1769.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes
    Authors: ZS Nevin, DC Factor, RT Karl, P Douvaras, J Laukka, MS Windrem, SA Goldman, V Fossati, GM Hobson, PJ Tesar
    Am. J. Hum. Genet., 2017;100(4):617-634.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Retinal Proteoglycans Act as Cellular Receptors for Basement Membrane Assembly to Control Astrocyte Migration and Angiogenesis
    Cell Rep, 2016;17(7):1832-1844.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Neuroprotective effects of oligodendrocyte progenitor cell transplantation in premature rat brain following hypoxic-ischemic injury.
    Authors: Chen, Long-Xia, Ma, Si-Min, Zhang, Peng, Fan, Zi-Chuan, Xiong, Man, Cheng, Guo-Qian, Yang, Yi, Qiu, Zi-Long, Zhou, Wen-Hao, Li, Jin
    PLoS ONE, 2015;10(3):e0115997.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Oligodendroglial maturation is dependent on intracellular protein shuttling.
    Authors: Gottle P, Sabo J, Heinen A, Venables G, Torres K, Tzekova N, Parras C, Kremer D, Hartung H, Cate H, Kury P
    J Neurosci, 2015;35(3):906-19.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Biologic effects of platelet-derived growth factor receptor alpha blockade in uterine cancer.
    Authors: Roh J, Huang J, Hu W, Yang X, Jennings N, Sehgal V, Sohn B, Han H, Lee S, Thanapprapasr D, Bottsford-Miller J, Zand B, Dalton H, Previs R, Davis A, Matsuo K, Lee J, Ram P, Coleman R, Sood A
    Clin Cancer Res, 2014;20(10):2740-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis.
    Authors: Hayes B, Riehle K, Shimizu-Albergine M, Bauer R, Hudkins K, Johansson F, Yeh M, Mahoney W, Yeung R, Campbell J
    PLoS ONE, 2014;9(3):e92925.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.
    Authors: Ball SG, Bayley C, Shuttleworth CA, Kielty CM
    Biochem. J., 2010;427(1):29-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
    Authors: Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ
    Brain, 2010;133(0):2232-47.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  26. A role for PDGF signaling in expansion of the extra-embryonic endoderm lineage of the mouse blastocyst.
    Authors: Artus J, Panthier JJ, Hadjantonakis AK
    Development, 2010;137(20):3361-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression.
    Authors: Wagsater D, Zhu C, Bjorck HM, Eriksson P
    Atherosclerosis, 2009;202(2):415-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Efficient Serum-Free Derivation of Oligodendrocyte Precursors from Neural Stem Cell-Enriched Cultures.
    Authors: Rao RC, Boyd J, Padmanabhan R, Chenoweth JG, McKay RD
    Stem Cells, 2008;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection.
    Authors: Soroceanu L, Akhavan A, Cobbs CS
    Nature, 2008;455(7211):391-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Musashi1 RNA-binding protein regulates oligodendrocyte lineage cell differentiation and survival.
    Authors: Dobson NR, Zhou YX, Flint NC, Armstrong RC
    Glia, 2008;56(3):318-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  32. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
    Authors: Li R, Maminishkis A, Wang FE, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2007;48(12):5722-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. PDGFR alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling.
    Authors: Jackson EL, Garcia-Verdugo JM, Gil-Perotin S, Roy M, Quinones-Hinojosa A, VandenBerg S, Alvarez-Buylla A
    Neuron, 2006;51(2):187-99.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  35. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Interferon-gamma inhibits transforming growth factor-beta production in human airway epithelial cells by targeting Smads.
    Authors: Wen FQ, Liu X, Kobayashi T, Abe S, Fang Q, Kohyama T, Ertl R, Terasaki Y, Manouilova L, Rennard SI
    Am. J. Respir. Cell Mol. Biol., 2004;30(6):816-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein.
    Authors: Mogi K, Goto M, Ohno E, Azumi Y, Takeuchi S, Toyoizumi R
    Int. J. Dev. Biol., 2003;47(1):15-29.
  38. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes.
    Authors: Nevin Z, Factor D, Karl R, Douvaras P, Laukka J, Windrem M, Goldman S, Fossati V, Hobson G, Tesar P
    Am J Hum Genet, 0;100(4):617-634.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human PDGF-AA Protein, CF

Average Rating: 4.5 (Based on 4 Reviews)

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Recombinant Human PDGF-AA Protein, CF
By Anonymous on 01/09/2020
Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: PDGF-AA Protein was used to differentiate human iPS cells into oligodendrocytes precursors. Performed reliably and consistently

Recombinant Human PDGF-AA Protein, CF
By Anonymous on 07/19/2018
Application: Stem/Immune cell maintenance or differentiation

Recombinant Human PDGF-AA Protein, CF
By Anonymous on 07/07/2018
Application: Stem/Immune cell maintenance or differentiation

Recombinant Human PDGF-AA Protein, CF
By Anonymous on 02/07/2018
Application: SDS-PAGE Control