Recombinant Human TSLPR Fc Chimera Avi-tag Protein, CF

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Recombinant Human TSLPR Fc Chimera Avi-tag Protein Binding Activity
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Recombinant Human TSLPR Fc Chimera Avi-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human TSLP (Catalog # 1398-TS) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human TSLP Fc Chimera Avi-tag (Catalog # AVI981) that produces 50% of the optimal binding response is 0.75-7.5 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human TSLPR protein
(Gly25 & Val30-Lys231)
Accession # Q9HC73.1
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly25 and Val30
Structure / Form
Biotinylated via Avi-tag
Predicted Molecular Mass
52 kDa
65-80 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human TSLPR Fc Chimera Avi-tag Protein Binding Activity View Larger

When Human TSLP Protein (Catalog # 1398-TS) is immobilized at 0.5 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human TSLPR Fc Chimera Avi-tag (Catalog # AVI981) with an ED50 of 0.75-7.5 ng/mL.

SDS-PAGE Recombinant Human TSLPR Fc Chimera Avi-tag Protein SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human TSLPR Fc Chimera Avi-tag (Catalog # AVI981) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa and 130-160 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: TSLPR

Thymic stromal lymphopoietin receptor (TSLPR), also known cytokine receptor-like factor 2 and IL-XR, is a member of the type 1 cytokine receptor family. TSLPR has been identified most closely related to the common gamma chain ( gamma c) and, when complexed with interleukin 7 receptor alpha (IL-7R alpha ), forms a high affinity complex for the IL-7-like cytokine TSLP (1-4). The extracellular domain (ECD) of human TSLPR contains two fibronectin type III-like domains and a WSXWS-like motif, which is necessary for proper protein folding (1-4). The cytoplasmic domain contains a membrane-proximal box 1 motif that is important for association with JAKs (1, 2). Human TSLPR shares 34% amino acid sequence identity with mouse TSLPR. An alternatively spliced mRNA variant encoding a soluble TSLPR has also been reported in mouse (5). In the signaling pathway of TSLP-TSLPR, which is similar to that of IL-7, TSLP activates the transcription factor signal transducer and activator of transcription 3 (STAT3), inducing the expression of common genes (4). TSLPR expression is ubiquitous in the immune and hematopoietic cells but is up‑regulated in Th2-skewed cells (3, 4). Elevated expression of TSLP-TSLPR in bronchial mucosa has been associated with human asthma by acting as a susceptibility factor to generate Th2 allergic responses to antigens (4, 6). TSLP also is involved in Th2-mediated allergic skin inflammation by inducing Th2 cytokine secretion by T cells during the effector phase of allergic skin inflammation (4, 7). TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (DC) (4). TSLP activated human DCs are also involved in the homeostatic proliferation of naïve and memory T cells in the absence of foreign antigens (4).

  1. Park LS et al. (2000) J. Exp. Med. 192:659.
  2. Blagoev, B. et al. (2002) Gene. 284:161.
  3. Ziegler, S.F. et al. (2013) Advances in Pharmacology. 66:129.
  4. He, R. et al. (2010) Ann N Y Acad Sci. 1183:13.
  5. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
  6. Headley, M.B. et al. (2009) J Imunol. 182:1641.
  7. Soumelis, V. et al. (2002) Nat Immunol. 3:673.
Long Name
Thymic Stromal Lymphopoietin Receptor
Entrez Gene IDs
64109 (Human); 57914 (Mouse); 171499 (Rat)
Alternate Names
CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR


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