Recombinant Human TSLPR Fc Chimera Avi-tag Protein, CF Summary
(Gly25 & Val30-Lys231)
Accession # Q9HC73.1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Human TSLP Protein (Catalog # 1398-TS) is immobilized at 0.5 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human TSLPR Fc Chimera Avi-tag (Catalog # AVI981) with an ED50 of 0.75-7.5 ng/mL.
2 μg/lane of Biotinylated Recombinant Human TSLPR Fc Chimera Avi-tag (Catalog # AVI981) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa and 130-160 kDa, respectively.
Thymic stromal lymphopoietin receptor (TSLPR), also known cytokine receptor-like factor 2 and IL-XR, is a member of the type 1 cytokine receptor family. TSLPR has been identified most closely related to the common gamma chain ( gamma c) and, when complexed with interleukin 7 receptor alpha (IL-7R alpha ), forms a high affinity complex for the IL-7-like cytokine TSLP (1-4). The extracellular domain (ECD) of human TSLPR contains two fibronectin type III-like domains and a WSXWS-like motif, which is necessary for proper protein folding (1-4). The cytoplasmic domain contains a membrane-proximal box 1 motif that is important for association with JAKs (1, 2). Human TSLPR shares 34% amino acid sequence identity with mouse TSLPR. An alternatively spliced mRNA variant encoding a soluble TSLPR has also been reported in mouse (5). In the signaling pathway of TSLP-TSLPR, which is similar to that of IL-7, TSLP activates the transcription factor signal transducer and activator of transcription 3 (STAT3), inducing the expression of common genes (4). TSLPR expression is ubiquitous in the immune and hematopoietic cells but is up‑regulated in Th2-skewed cells (3, 4). Elevated expression of TSLP-TSLPR in bronchial mucosa has been associated with human asthma by acting as a susceptibility factor to generate Th2 allergic responses to antigens (4, 6). TSLP also is involved in Th2-mediated allergic skin inflammation by inducing Th2 cytokine secretion by T cells during the effector phase of allergic skin inflammation (4, 7). TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (DC) (4). TSLP activated human DCs are also involved in the homeostatic proliferation of naïve and memory T cells in the absence of foreign antigens (4).
- Park LS et al. (2000) J. Exp. Med. 192:659.
- Blagoev, B. et al. (2002) Gene. 284:161.
- Ziegler, S.F. et al. (2013) Advances in Pharmacology. 66:129.
- He, R. et al. (2010) Ann N Y Acad Sci. 1183:13.
- Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
- Headley, M.B. et al. (2009) J Imunol. 182:1641.
- Soumelis, V. et al. (2002) Nat Immunol. 3:673.
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