Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1356-SR-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD117/c-kit Fc Chimera is present at 0.5 μg/mL, the concentration of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC) that produces 50% of the optimal binding response is approximately 3‑12 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD117/c-kit protein
Mouse CD117/c-kit
(Gln26-Thr523)
Accession # P05532
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gln26 predicted: No results obtained, sequencing might be blocked
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
82.6 kDa (monomer)
SDS-PAGE
100-125 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1356-SR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD117/c-kit

Stem Cell Factor Receptor (SCF R), also known as c‑Kit and CD117, is a widely expressed 145 kDa receptor tyrosine kinase. It is the cellular homolog of the feline sarcoma virus protein, v‑Kit. Binding of SCF R to SCF, also known as Steel Factor and Kit Ligand, promotes the survival, differentiation, and mobilization of progenitor cells in multiple lineages (1‑4). Mutations or deletions of SCF R cause a wide variety of malignancies as well as pigmentation disorders and sterility (5, 6). Mature mouse SCF R consists of a 503 amino acid (aa) extracellular domain (ECD) with five tandem immunoglobulin‑like domains, a 21 aa transmembrane segment, and a 431 aa cytoplasmic domain with the split tyrosine kinase domain (7). Within the ECD, mouse SCF R shares 73% and 88% aa sequence identity with human and rat SCF R, respectively. Alternative splicing of mouse SCF R generates a truncated intracellular isoform that corresponds to the C‑terminal half of the tyrosine kinase domain (8). SCF is expressed as transmembrane and soluble noncovalent homodimers (9). One SCF dimer binds to two molecules of SCF R, inducing receptor dimerization and activation (9). Transmembrane SCF induces more prolonged signaling through SCF R compared to soluble SCF (10). Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (11). A 100 kDa glycosylated ECD fragment of SCF R can be shed into the circulation by TACE/ADAM17, and this fragment inhibits the interaction of SCF with transmembrane SCF R (12, 13). SCF is a primary growth and activation factor for mast cells and eosinophils (14). SCF R expression on mast cells enables them to infiltrate SCF‑secreting tumors where they promote tumor growth and induce local immune suppression (15). SCF R is up‑regulated on dendritic cells by Th2‑ or Th17‑biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (16). SCF R protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (17, 18).

References
  1. Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
  2. Tallini, Y.N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1808.
  3. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
  4. Sun, L. et al. (2004) J. Clin. Invest. 113:1364.
  5. Pittoni, P. et al. (2011) Oncogene 30:757.
  6. Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.
  7. Qiu, F.H. et al. (1988) EMBO J. 7:1003.
  8. Zayas, J. et al. (2008) Stem Cells Dev. 17:343.
  9. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  10. Miyazawa, K. et al. (1995) Blood 85:641.
  11. Martin, F.H. et al. (1990) Cell 63:203.
  12. Turner, A.M. et al. (1995) Blood 85:2052.
  13. Cruz, A.C. et al. (2004) J. Biol. Chem. 279:5612.
  14. Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
  15. Huang, B. et al. (2008) Blood 112:1269.
  16. Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
  17. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  18. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
Long Name
Stem Cell Factor Receptor
Entrez Gene IDs
3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Citation for Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling
    Authors: M Schmitt, M Schewe, A Sacchetti, D Feijtel, WS van de Gee, M Teeuwssen, HF Sleddens, R Joosten, ME van Royen, HJG van de Wer, J van Es, H Clevers, R Fodde
    Cell Rep, 2018;24(9):2312-2328.e7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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