Recombinant Mouse ErbB3/Her3 Fc Chimera Protein, CF
Recombinant Mouse ErbB3/Her3 Fc Chimera Protein, CF Summary
(Ser20 - His641)
Accession # Q61526
(Glu98 - Lys330)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
ErbB3, also called HER3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors named for a viral oncogene (1 - 3). ErbB family members serve as receptors for the EGF family of growth factors (1 - 3). Mouse ErbB3 contains a 19 amino acid (aa) signal sequence, a 622 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 677 aa cytoplasmic domain (4). Human ErbB3 has four isoforms created by intron read-through and truncation of the molecule (5). Three of these are secreted and at least one can inhibit ErbB3 activity (6). Little information is available concerning mouse ErbB3 isoforms. The mouse ErbB3 ECD shares 97%, 93%, 92%, 91%, 89% and 88% aa identity with rat, human, bovine, equine, canine and opossum ErbB3, respectively. ErbB3 is found in epithelial cell layers of gastrointestinal, reproductive, urinary, endocrine and nervous systems, skin and muscle (3). Among ErbB family members, only ErbB3 lacks a working kinase domain, requiring heterodimerization with another ErbB receptor for signaling (1 - 3). The heterodimer of ErbB3 with ErbB2, which has no known ligands of its own, is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2 (3, 7 - 9). These ligands also bind ErbB4 (1). Signaling is aided by the six consensus binding motifs for the SH2 domain and one for the SH3 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (10, 11). Deletion studies in mice demonstrate non-redundant roles for ErbB3 in development of Schwann cells, neural crest cells and heart valves (12, 13). ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system (14).
- Linggi, B. and G. Carpenter (2006) Trends Cell Biol. 16:649.
- Citri, A. and Y. Yarden (2006) Nat. Rev. Mol. Cell Biol. 7:505.
- Citri, A. et al. (2003) Exp. Cell Res. 284:54.
- Swissprot Accession # Q61526.
- Lee, H. et al. (1998) Oncogene 16:3243.
- Lee, H. et al. (2001) Cancer Res. 61:4467.
- Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
- Sundaresan, S. et al. (1998) Endocrinology 139:4756.
- Wallasch, C. et al. (1995) EMBO J. 14:4267.
- Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
- Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
- Riethmacher, D. et al. (1997) Nature 389:725.
- Erickson, S.L. et al. (1997) Development 124:4999.
- Britsch, S. et al (1998) Genes Dev. 12:1825.
Citations for Recombinant Mouse ErbB3/Her3 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Inhibition of HER3 activation and tumor growth with a human antibody binding to a conserved epitope formed by domain III and IV
Authors: LC Schmitt, A Rau, O Seifert, J Honer, M Hutt, S Schmid, J Zantow, M Hust, S Dübel, MA Olayioye, RE Kontermann
ErbB2 directly activates the exchange factor Dock7 to promote Schwann cell migration.
Authors: Yamauchi J, Miyamoto Y, Chan JR, Tanoue A
J. Cell Biol., 2008-04-21;181(2):351-65.
Sample Types: Whole Cells
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Mouse ErbB3/Her3 Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse ErbB3/Her3 Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Mouse ErbB3/Her3 Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image