Recombinant Mouse IL-17E Protein

Newer Version Available: 7909-IL
A new and Improved rm IL-17E is available! It is NS0 expressed and has ~2 fold better activity!
Formulations:
Catalog # Availability Size / Price Qty
1399-IL-025
Product Details
Citations (36)
FAQs
Reviews (2)

Recombinant Mouse IL-17E Protein Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.25-1.5 ng/mL.
Source
E. coli-derived mouse IL-17E/IL-25 protein
Val17-Ala169, with and without an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Val17 and Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
17.6 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1399-IL

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1399-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: IL-17E/IL-25

The Interleukin‑17 (IL‑17) family of proteins are immunoregulatory cytokines that share a conserved cysteine‑rich region. IL‑17E, which is also known as IL‑25, promotes Th2‑biased immune responses. This is in contrast to other IL‑17 family members which promote Th1‑ and Th17‑biased inflammation. IL‑25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature mouse IL‑25 shares 80% and 91% amino acid sequence identity with human and rat IL‑25, respectively (3, 4). During helminth infections and allergic reactions, IL‑25 is locally up‑regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4‑9). It binds to IL‑17 RB but also requires IL‑17 RA to exert its activity (3, 8, 10). IL‑25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL‑4, IL‑5, IL‑13) and reduced production of Th1 and Th17 cytokines (e.g. IFN‑ gamma, IL‑12, IL‑23, IL‑17A, IL‑17F) (4‑6, 8, 9, 11‑15). Airway IL‑25 can be activated by MMP‑7, a protease that is up‑regulated in airway epithelium in response to allergen exposure (16). Cleaved IL‑25 shows enhanced binding to IL‑17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and anti‑inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective anti‑helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL‑25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis (12, 15). IL‑25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood‑brain barrier and limits CD4+ T cell infiltration into the brain (17).

References
  1. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
  2. Iwakura, Y. et al. (2011) Immunity 34:149.
  3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  4. Fort, M.M. et al. (2001) Immunity 15:985.
  5. Zhao, A. et al. (2010) J. Immunol. 185:6921.
  6. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
  7. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
  8. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
  9. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
  10. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
  11. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  12. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
  13. Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
  14. Stock, P. et al. (2009) J. Immunol. 182:5116.
  15. Caruso, R. et al. (2009) Gastroenterology 136:2270.
  16. Goswami, S. et al. (2009) Nat. Immunol. 10:496.
  17. Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.
Long Name
Interleukin 17E
Entrez Gene IDs
64806 (Human); 140806 (Mouse); 501996 (Rat)
Alternate Names
IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25

Citations for Recombinant Mouse IL-17E Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions
    Authors: PT Mantani, P Dunér, I Ljungcrant, J Nilsson, H Björkbacka, GN Fredrikson
    BMC Immunol., 2019;20(1):47.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. IL-25 enhances Th17 cell-mediated contact dermatitis by promoting IL-1? production by dermal dendritic cells
    Authors: H Suto, A Nambu, H Morita, S Yamaguchi, T Numata, T Yoshizaki, E Shimura, K Arae, Y Asada, K Motomura, M Kaneko, T Abe, A Matsuda, Y Iwakura, K Okumura, H Saito, K Matsumoto, K Sudo, S Nakae
    J. Allergy Clin. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Thymic tuft cells promote an IL-4-enriched medulla and shape thymocyte development
    Authors: CN Miller, I Proekt, J von Moltke, KL Wells, AR Rajpurkar, H Wang, K Rattay, IS Khan, TC Metzger, JL Pollack, AC Fries, WW Lwin, EJ Wigton, AV Parent, B Kyewski, DJ Erle, KA Hogquist, LM Steinmetz, RM Locksley, MS Anderson
    Nature, 2018;559(7715):627-631.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Neuronal regulation of type 2 innate lymphoid cells via neuromedin U
    Authors: V Cardoso, J Chesné, H Ribeiro, B García-Cas, T Carvalho, T Bouchery, K Shah, NL Barbosa-Mo, N Harris, H Veiga-Fern
    Nature, 2017;549(7671):277-281.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Role of Eosinophils and Tumor Necrosis Factor Alpha in Interleukin-25-Mediated Protection from Amebic Colitis
    Authors: Z Noor, K Watanabe, MM Abhyankar, SL Burgess, EL Buonomo, CA Cowardin, WA Petri
    MBio, 2017;8(1):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice
    Authors: M Han, C Rajput, JY Hong, J Lei, JL Hinde, Q Wu, JK Bentley, MB Hershenson
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Type 2 innate lymphoid cells treat and prevent acute gastrointestinal graft-versus-host disease
    Authors: DW Bruce, HE Stefanski, BG Vincent, TA Dant, S Reisdorf, H Bommiasamy, DA Serody, JE Wilson, KP McKinnon, WD Shlomchik, PM Armistead, JP Ting, JT Woosley, BR Blazar, DM Zaiss, AN McKenzie, JM Coghill, JS Serody
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Interleukin-25 Induces Resistance Against Intestinal Trematodes
    Sci Rep, 2016;6(0):34142.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. IL-25 attenuates rheumatoid arthritis through suppression of Th17 immune responses in an IL-13-dependent manner
    Sci Rep, 2016;6(0):36002.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. A critical role for IL-25 in host protective Th2 memory response against Heligmosomoides polygyrus bakeri
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. The ETS1 transcription factor is required for the development and cytokine-induced expansion of ILC2
    Authors: EC Zook, K Ramirez, X Guo, G van der Vo, M Sigvardsso, EC Svensson, YX Fu, BL Kee
    J Exp Med, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. IL-25 or IL-17E Protects against High-Fat Diet-Induced Hepatic Steatosis in Mice Dependent upon IL-13 Activation of STAT6.
    Authors: Wang A, Yang Z, Grinchuk V, Smith A, Qin B, Lu N, Wang D, Wang H, Ramalingam T, Wynn T, Urban J, Shea-Donohue T, Zhao A
    J Immunol, 2015;195(10):4771-80.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  13. IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model.
    Authors: Yao, Xiujuan, Wang, Wei, Li, Yan, Huang, Ping, Zhang, Qian, Wang, Jingjing, Wang, Wen, Lv, Zhe, An, Yunqing, Qin, Jianguo, Corrigan, Chris J, Huang, Kewu, Sun, Yongchan, Ying, Sun
    Respir Res, 2015;16(0):39.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. Collaborative interactions between type 2 innate lymphoid cells and antigen-specific CD4+ Th2 cells exacerbate murine allergic airway diseases with prominent eosinophilia.
    Authors: Liu B, Lee J, Chen C, Hershey G, Wang Y
    J Immunol, 2015;194(8):3583-93.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. Cutting Edge: IL-25 Targets Dendritic Cells To Attract IL-9-Producing T Cells in Acute Allergic Lung Inflammation.
    Authors: Claudio E, Tassi I, Wang H, Tang W, Ha H, Siebenlist U
    J Immunol, 2015;195(8):3525-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Interleukin-17B Antagonizes Interleukin-25-Mediated Mucosal Inflammation.
    Authors: Reynolds J, Lee Y, Shi Y, Wang X, Angkasekwinai P, Nallaparaju K, Flaherty S, Chang S, Watarai H, Dong C
    Immunity, 2015;42(4):692-703.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. IL-25 inhibits atherosclerosis development in apolipoprotein E deficient mice.
    Authors: Mantani P, Duner P, Bengtsson E, Alm R, Ljungcrantz I, Soderberg I, Sundius L, To F, Nilsson J, Bjorkbacka H, Fredrikson G
    PLoS ONE, 2015;10(1):e0117255.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  18. TNF superfamily member TL1A elicits type 2 innate lymphoid cells at mucosal barriers.
    Authors: Yu, X, Pappu, R, Ramirez-Carrozzi, V, Ota, N, Caplazi, P, Zhang, J, Yan, D, Xu, M, Lee, W P, Grogan, J L
    Mucosal Immunol, 2014;7(3):730-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  19. Control of T helper 2 responses by transcription factor IRF4-dependent dendritic cells.
    Authors: Gao Y, Nish S, Jiang R, Hou L, Licona-Limon P, Weinstein J, Zhao H, Medzhitov R
    Immunity, 2013;39(4):722-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Macrophages as IL-25/IL-33-responsive cells play an important role in the induction of type 2 immunity.
    Authors: Yang Z, Grinchuk V, Urban J, Bohl J, Sun R, Notari L, Yan S, Ramalingam T, Keegan A, Wynn T, Shea-Donohue T, Zhao A
    , 2013;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  21. Pulmonary innate lymphoid cells are major producers of IL-5 and IL-13 in murine models of allergic asthma.
    Authors: Wolterink RG, KleinJan A, van Nimwegen M, Bergen I, de Bruijn M, Levani Y, Hendriks RW
    Eur. J. Immunol., 2012;42(5):1106-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. Mast cells orchestrate type 2 immunity to helminths through regulation of tissue-derived cytokines.
    Authors: Hepworth MR, Danilowicz-Luebert E, Rausch S, Metz M, Klotz C, Maurer M, Hartmann S
    Proc. Natl. Acad. Sci. U.S.A., 2012;109(17):6644-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, Is crucial for murine asthma.
    Authors: Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Korner H, Cua D, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide D, Matsumoto K, Nakae S
    J Immunol, 2012;189(7):3641-52.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  24. RORgammat+ innate lymphoid cells regulate intestinal homeostasis by integrating negative signals from the symbiotic microbiota.
    Authors: Sawa S, Lochner M, Satoh-Takayama N, Dulauroy S, Berard M, Kleinschek M, Cua D, Di Santo JP, Eberl G
    Nat. Immunol., 2011;12(4):320-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  25. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
    Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
    J. Immunol., 2011;188(3):1503-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Intravenous gammaglobulin suppresses inflammation through a novel Th2 pathway.
    Authors: Anthony RM, Kobayashi T, Wermeling F, Ravetch JV
    Nature, 2011;475(7354):110-3.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  27. T cell-derived Act1 is necessary for IL-25-mediated Th2 responses and allergic airway inflammation.
    Authors: Swaidani S, Bulek K, Kang Z
    J. Immunol., 2011;187(6):3155-64.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  28. Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice.
    Authors: Nel HJ, Hams E, Saunders SP
    J. Immunol., 2011;186(8):4631-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  29. Critical role of IL-25 in nematode infection-induced alterations in intestinal function.
    Authors: Zhao A, Urban JF, Sun R
    J. Immunol., 2010;185(0):6921-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  30. Systemically dispersed innate IL-13-expressing cells in type 2 immunity.
    Authors: Price AE, Liang HE, Sullivan BM
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(25):11489-94.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  31. Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity.
    Authors: Neill DR, Wong SH, Bellosi A, Flynn RJ, Daly M, Langford TK, Bucks C, Kane CM, Fallon PG, Pannell R, Jolin HE, McKenzie AN
    Nature, 2010;464(7293):1367-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  32. Divergent functions for airway epithelial matrix metalloproteinase 7 and retinoic acid in experimental asthma.
    Authors: Goswami S, Angkasekwinai P, Shan M, Greenlee KJ, Barranco WT, Polikepahad S, Seryshev A, Song LZ, Redding D, Singh B, Sur S, Woodruff P, Dong C, Corry DB, Kheradmand F
    Nat. Immunol., 2009;10(5):496-503.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  33. The critical role of epithelial-derived Act1 in IL-17- and IL-25-mediated pulmonary inflammation.
    Authors: Swaidani S, Bulek K, Kang Z, Liu C, Lu Y, Yin W, Aronica M, Li X
    J. Immunol., 2009;182(3):1631-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  34. The adaptor protein CIKS/Act1 is essential for IL-25-mediated allergic airway inflammation.
    Authors: Claudio E, Sonder SU, Saret S, Carvalho G, Ramalingam TR, Wynn TA, Chariot A, Garcia-Perganeda A, Leonardi A, Paun A, Chen A, Ren NY, Wang H, Siebenlist U
    J. Immunol., 2009;182(3):1617-30.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  35. MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.
    Authors: Perrigoue JG, Saenz SA, Siracusa MC, Allenspach EJ, Taylor BC, Giacomin PR, Nair MG, Du Y, Zaph C, Van Rooijen N, Comeau MR, Pearce EJ, Laufer TM, Artis D
    Nat. Immunol., 2009;10(7):697-705.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  36. IL-25 enhances allergic airway inflammation by amplifying a TH2 cell-dependent pathway in mice.
    Authors: Tamachi T, Maezawa Y, Ikeda K, Kagami S, Hatano M, Seto Y, Suto A, Suzuki K, Watanabe N, Saito Y, Tokuhisa T, Iwamoto I, Nakajima H
    J. Allergy Clin. Immunol., 2006;118(3):606-14.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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Reviews for Recombinant Mouse IL-17E Protein

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Mouse IL-17E Protein
By Anonymous on 12/18/2019
Application: Stimulating BMDM with IL-25. Remaining IL-25 can be detected (quality checked) by ELISA (see image).
Reason for Rating: Product works very well in assay used for stimulating macrophages in vitro. Product very stable in cell culture and can be readily detected by ELISA.

Recombinant Mouse IL-17E Protein
By Anonymous on 07/29/2016
Application: Binding assay/Protein-protein interaction
Reason for Rating: Worked well in in invitro assay. Activity seems to be a bit weaker than human counterpart.