Recombinant Mouse Integrin alpha 4 beta 1 Protein, CF

Catalog # Availability Size / Price Qty
6054-A4-050
Product Details
Citations (1)
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Recombinant Mouse Integrin alpha 4 beta 1 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. When 5 x 104 cells/well are added to Recombinant Mouse Integrin alpha 4 beta 1 coated plates (10 μg/mL, 100 μL/well), approximately 55-85% will adhere after 1 hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 4 beta 1 protein
Mouse Integrin alpha 4
Tyr34-Gln970 (Arg591Leu) 
Accession # AAH68313
GGGSGGGSGGGSAcidic Tail 6-His tag
Mouse Integrin beta 1
(Gln2-Asp728)
Accession # P09055
GGGSGGGSGGGS Basic Tail
N-terminusC-terminus
N-terminal Sequence
Analysis
Tyr34 (Integrin alpha 4) & No results obtained: Gln21 predicted (Integrin beta 1)
Predicted Molecular Mass
112.9 kDa (Integrin alpha 4) & 86.4 kDa (Integrin beta 1)
SDS-PAGE
145-160 kDa and 120-140 kDa, reducing donditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6054-A4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Integrin alpha 4 beta 1

Integrin alpha 4 beta 1, also called VLA4, is an integrin family adhesion receptor that shares the beta 1 subunit with eleven other family members and the alpha 4 subunit with Integrin  alpha 4 beta 7 (1-4). The non-covalent heterodimer of 150 kDa alpha 4/CD49d and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits mediates cell adhesion to VCAM‑1/CD106 on other cells and the CS-1 fragment of fibronectin in the extracellular matrix (2-4). The alpha 4 extracellular domain (ECD) contains an N-terminal beta ‑propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 4 beta -propeller to form a binding domain when the dimer is in active, extended and open conformation. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The 944 aa mouse alpha 4 ECD shares 85% and 94% aa identity with human and rat alpha 4, respectively, while the 708 aa mouse beta 1 ECD shares 98% aa identity with rat and 93-94% aa identity with human, bovine, porcine, ovine, canine and feline beta 1. Leukocytes (except for neutrophils), erythroid precursors and some non-hematopoietic cells such as epicardial, endothelial and smooth muscle precursors, Schwann cells, and chorionic cells express alpha 4 beta 1 (5-9). Deletion is lethal in the mouse embryo due to faulty placentation and development of the epicardium and coronary vessels (6, 9). In the adult, alpha 4 beta 1 primarily regulates immune cell migration (10-12). Circulating leukocyte alpha 4 beta 1 is rapidly activated by inflamed endothelial cells that present VCAM-1 and chemokines such as SDF-1 (10). This activation facilitates rolling, firm adhesion, and extravasation. In humans, interfering with leukocyte migration via the therapeutic alpha 4 beta 1antibody Natalizumab can reduce the severity of autoimmune disorders such as multiple sclerosis (11). Natalizumab can also mobilize hematopoietic precursors from the bone marrow by impeding their interaction with stromal cell VCAM-1 (7, 11).  During immune cell activation, alpha 4 beta 1 can function as a costimulatory molecule (12, 13).

References
  1. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  2. Takada, Y. et al. (1989) EMBO J. 8:1361.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 1025:1183.
  4. Takada, Y. et al. (2007) Genome Biol. 8:215.
  5. Eshghi, S. et al. (2007) J. Cell Biol. 177:871.
  6. Yang, J.T. et al. (1995) Development 121:549.
  7. Sheppard, A.M. et al. (1994) Cell Adhes. Commun. 2:27.
  8. Haack, H. and R.O. Hynes (2001) Dev. Biol. 233:38.
  9. Grazioli, A. et al. (2006) Dev. Biol. 293:165.
  10. Alon, R. and K. Ley (2008) Curr. Opin. Cell Biol. 20:525.
  11. Bauer, M. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1920.
  12. Banerjee, E.R. et al. (2008) Exp. Hematol. 36:1004.
  13. Nguyen, K. et al. (2008) Immunity 28:810.
Entrez Gene IDs
3676 (Human)
Alternate Names
Integrin alpha 4 beta 1

Citation for Recombinant Mouse Integrin alpha 4 beta 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The role of glycoprotein H of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in cellular host range and integrin binding.
    Authors: Azab W, Zajic L, Osterrieder N
    Vet Res, 2012;43(1):61.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay

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