Recombinant Mouse LIF Protein, CF

(2 citations)   
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 for this effect is 0.1-0.6 ng/mL.
  • Source
    E. coli-derived Pro25-Phe203
  • Accession #
  • N-terminal Sequence
    Analysis
    Pro25
  • Predicted Molecular Mass
    20 kDa
  • SDS-PAGE
    20 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8878-LF/CF
 
8878-LF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 0.2-1 mg/mL in sterile PBS.
Reconstitution Reconstitute at 0.2-1 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Mouse Embryonic Stem Cells Cultured in Recombinant Mouse LIF Maintain their Pluripotency.
D3 mouse ESCs were cultured in Recombinant Mouse LIF (10 ng/mL) for 15 days, including 4 passages. ESCs continue to express the mouse pluripotency markers, SSEA-1 (red) and SOX2 (green). SSEA-1 was visualized with the Human/Mouse SSEA-1 NorthernLights™ (NL)557-conjugated Antibody (Catalog # NL2155R). SOX2 was visualized with the Anti-Human SOX2 Antibody (Catalog # AF2018) followed by the Donkey Anti-Goat NL493 (Catalog # NL003). The cell nuclei were stained with DAPI (blue).
Mouse LIF Stimulates IL-6 Secretion
Recombinant Mouse LIF(Catalog # 8878-LF) inducesIL-6 secretion in M1 mouse myeloid leukemia cells. The ED50 for for this effect is 0.1-0.6ng/mL
Recombinant Mouse LIF Promotes the Expansion of Mouse Embryonic Stem Cells. 
D3 mouse ESCs were cultured with Recombinant Mouse LIF (10 ng/mL). After 15 days, and following multiple rounds of passaging, the ESCs were analyzed for pluripotency by staining with PE-conjugated anti-SSEA-1 (Catalog# FAB2155P) and APC-conjugated SSEA-4 (Catalog # FAB1435A). ESCs continue to express the pluripotency marker SSEA-1 and lack expression of SSEA-4, a negative marker for mouse ESCs. Quadrants were set based on samples stained with isotype control antibodies.
Background: LIF
LIF (leukemia inhibitory factor) is a widely expressed pleiotropic member of the IL-6 family of cytokines (1-3). Mature mouse LIF is expressed as a highly and variably glycosylated 32-62 kDa monomer that shares 78%, 91%, 80%, 76%, and 78% aa sequence identity with human, rat, canine, bovine, and porcine LIF, respectively (4). LIF functions through a heterodimeric receptor complex containing a ligand-binding subunit, LIF R alpha /CD118, and a signal transducing subunit, gp130 (2, 4, 5). gp130 also serves as a subunit of the receptor complexes for Oncostatin M, Cardiotrophin-1, CNTF, IL-6, IL-11, and IL-27 (2, 5). A soluble form of mouse LIF R alpha can be generated by alternative splicing (6). Depending on the cells and their context, LIF either opposes or favors differentiation (2, 7). LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells (2, 5, 7). However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation (2, 5). LIF is produced by activated CD4+ T cells and is required by the thymic epithelium to support T cell maturation (2, 3). Its expression is upregulated by neuronal injury, and it promotes motor neuron survival and oligodendrocyte myelination (2, 3, 8). It is produced by the adrenal cortex and likely enhances adrenal production of cortisol and aldosterone (9). LIF can also function as an autocrine growth factor in some pancreatic cancers, but it induces differentiation in the myeloid leukemic cell line M1 (1, 10). Tumor cell-derived LIF can also induce formation of immunosuppressive tumor-associated macrophages (11). LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells (2, 3, 12). It promotes regulatory T cell and inhibits Th17 cell differentiation, thus down-regulating inflammation and contributing to immune tolerance during pregnancy and in the nervous system (2, 3, 5, 7).
  • References:
    1. Moreau, J.F. et al. (1988) Nature 336:690.
    2. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51.
    3. Metcalfe, S.M. (2011) Genes Immun. 12:157.
    4. Gearing, D.P. et al. (1987) EMBO J. 6:3995.
    5. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
    6. Tomida, M. et al. (1993) FEBS lett. 334:193.
    7. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
    8. Slaets, H. et al. (2010) Trends Mol. Med. 16:493.
    9. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145.
    10. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977.
    11. Duluc, D. et al. (2007) Blood 110:4319.
    12. Zouein, F.A. et al. (2013) Eur. Cytokine Netw. 24:11.
  • Long Name:
    Leukemia Inhibitory Factor
  • Entrez Gene IDs:
    3976 (Human); 16878 (Mouse); 403449 (Canine)
  • Alternate Names:
    CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Cancer stem cells regulate cancer-associated fibroblasts via activation of Hedgehog signaling in mammary gland tumors
    Authors: G Valenti, HM Quinn, GJ Heynen, L Lan, JD Holland, R Vogel, A Wulf-Golde, W Birchmeier
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Renalase expression by melanoma and tumor associated-macrophages promotes tumor growth through a STAT3-mediated mechanism
    Cancer Res, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay

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