Recombinant Mouse LIF Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
8878-LF-025/CF
8878-LF-100/CF
8878-LF-500/CF
Recombinant Mouse LIF Protein, CF Bioactivity
3 Images
Product Details
Citations (1)
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Recombinant Mouse LIF Protein, CF Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 for this effect is 0.1-0.6 ng/mL.
Source
E. coli-derived mouse LIF protein
Pro25-Phe203
Accession #
N-terminal Sequence
Analysis
Pro25
Predicted Molecular Mass
20 kDa
SDS-PAGE
20 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8878-LF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 0.2-1 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8878-LF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 0.2-1 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Mouse LIF Protein, CF Bioactivity View Larger

D3 mouse ESCs were cultured in Recombinant Mouse LIF (10 ng/mL) for 15 days, including 4 passages. ESCs continue to express the mouse pluripotency markers, SSEA-1 (red) and SOX2 (green). SSEA-1 was visualized with the Human/Mouse SSEA-1 NorthernLights™ (NL)557-conjugated Antibody (Catalog # NL2155R). SOX2 was visualized with the Anti-Human SOX2 Antibody (Catalog # AF2018) followed by the Donkey Anti-Goat NL493 (Catalog # NL003). The cell nuclei were stained with DAPI (blue).

Bioactivity Recombinant Mouse LIF Protein, CF Bioactivity View Larger

Recombinant Mouse LIF(Catalog # 8878-LF) inducesIL-6 secretion in M1 mouse myeloid leukemia cells. The ED50 for for this effect is 0.1-0.6ng/mL

Bioactivity Recombinant Mouse LIF Protein, CF Bioactivity View Larger

D3 mouse ESCs were cultured with Recombinant Mouse LIF (10 ng/mL). After 15 days, and following multiple rounds of passaging, the ESCs were analyzed for pluripotency by staining with PE-conjugated anti-SSEA-1 (Catalog# FAB2155P) and APC-conjugated SSEA-4 (Catalog # FAB1435A). ESCs continue to express the pluripotency marker SSEA-1 and lack expression of SSEA-4, a negative marker for mouse ESCs. Quadrants were set based on samples stained with isotype control antibodies.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIF

LIF (leukemia inhibitory factor) is a widely expressed pleiotropic member of the IL-6 family of cytokines (1-3). Mature mouse LIF is expressed as a highly and variably glycosylated 32-62 kDa monomer that shares 78%, 91%, 80%, 76%, and 78% aa sequence identity with human, rat, canine, bovine, and porcine LIF, respectively (4). LIF functions through a heterodimeric receptor complex containing a ligand-binding subunit, LIF R alpha /CD118, and a signal transducing subunit, gp130 (2, 4, 5). gp130 also serves as a subunit of the receptor complexes for Oncostatin M, Cardiotrophin-1, CNTF, IL-6, IL-11, and IL-27 (2, 5). A soluble form of mouse LIF R alpha can be generated by alternative splicing (6). Depending on the cells and their context, LIF either opposes or favors differentiation (2, 7). LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells (2, 5, 7). However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation (2, 5). LIF is produced by activated CD4+ T cells and is required by the thymic epithelium to support T cell maturation (2, 3). Its expression is upregulated by neuronal injury, and it promotes motor neuron survival and oligodendrocyte myelination (2, 3, 8). It is produced by the adrenal cortex and likely enhances adrenal production of cortisol and aldosterone (9). LIF can also function as an autocrine growth factor in some pancreatic cancers, but it induces differentiation in the myeloid leukemic cell line M1 (1, 10). Tumor cell-derived LIF can also induce formation of immunosuppressive tumor-associated macrophages (11). LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells (2, 3, 12). It promotes regulatory T cell and inhibits Th17 cell differentiation, thus down-regulating inflammation and contributing to immune tolerance during pregnancy and in the nervous system (2, 3, 5, 7).

References
  1. Moreau, J.F. et al. (1988) Nature 336:690.
  2. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51.
  3. Metcalfe, S.M. (2011) Genes Immun. 12:157.
  4. Gearing, D.P. et al. (1987) EMBO J. 6:3995.
  5. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
  6. Tomida, M. et al. (1993) FEBS lett. 334:193.
  7. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  8. Slaets, H. et al. (2010) Trends Mol. Med. 16:493.
  9. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145.
  10. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977.
  11. Duluc, D. et al. (2007) Blood 110:4319.
  12. Zouein, F.A. et al. (2013) Eur. Cytokine Netw. 24:11.
Long Name
Leukemia Inhibitory Factor
Entrez Gene IDs
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names
CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI

Citation for Recombinant Mouse LIF Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210
    Authors: Y Tang, S Xiong, P Yu, F Liu, L Cheng
    Cell Rep, 2018;24(5):1355-1362.e3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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