Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian Noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse Noggin shares 99% and 83% aa sequence identity with human and Xenopus Noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, Noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.
Recombinant Mouse Noggin Protein
R&D Systems | Catalog # 1967-NG
Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Noggin Protein (1967-NG)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
| Mouse Noggin (Leu20-Cys232) Accession # P97466 |
IEGRGGGSGGGSGGGS | 10-His tag |
| N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 0.06-0.3 µg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).
Reviewed Applications
Read 3 reviews rated 4.3 using 1967-NG in the following applications:
Scientific Data Images for Recombinant Mouse Noggin Protein
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1967-NG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1967-NG/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: Noggin
References
- Smith, W.C. and R.M. Harland (1992) Cell 70:829.
- Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
- Brunet, L.J. et al. (1998) Science 280:1455.
Alternate Names
Gene Symbol
UniProt
Additional Noggin Products
Product Documents for Recombinant Mouse Noggin Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Noggin Protein
For research use only
Related Research Areas
Citations for Recombinant Mouse Noggin Protein
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Customer Reviews for Recombinant Mouse Noggin Protein (3)
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 06/16/2020
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Application: Cell ProliferationVerified Customer | Posted 09/08/2017
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Application: Cell migration/motilityVerified Customer | Posted 05/18/2016
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