Recombinant Rat PDGF-BB Protein

Carrier Free

Catalog # Availability Size / Price Qty
520-BB-050/CF

With Carrier

Catalog # Availability Size / Price Qty
520-BB-050
Graph showing dose-dependent bioactivity of rat PDGF-BB protein
1 Image
Product Details
Citations (29)
FAQs
Reviews

Recombinant Rat PDGF-BB Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5‑6 ng/mL.
Source
E. coli-derived rat PDGF-BB protein
Ser74-Thr182
Accession #
N-terminal Sequence
Analysis
Ser74
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
25 kDa

Product Datasheets

520-BB (with carrier)

520-BB/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

520-BB

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

520-BB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity of Rat PDGF-BB View Larger

Recombinant rat PDGF-BB (520-BB) induces proliferation in the NR6R-3T3 mouse fibroblast cell line. The ED50 for this effect is 1.5-6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the
alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production; temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

References
  1. Raines, E.W. et al. (1990) Platelet-derived growth factor in Handbook of Experimental Pharmacology, Peptide growth factors and their receptors, M.B. Sporn and A.B. Roberts, eds. p. 173.
Long Name
Platelet-derived Growth Factor BB
Entrez Gene IDs
5155 (Human); 24628 (Rat)
Alternate Names
PDGFBB; PDGF-BB

Citations for Recombinant Rat PDGF-BB Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Periprocedural Hydrogen Sulfide Therapy Improves Vascular Remodeling and Attenuates Vein Graft Disease
    Authors: P Kip, M Tao, KM Trocha, MR MacArthur, HAB Peters, SJ Mitchell, CG Mann, TJ Sluiter, J Jung, S Patterson, PHA Quax, MR de Vries, JR Mitchell, C Keith Ozak
    J Am Heart Assoc, 2020;0(0):e016391.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Gsta4 controls apoptosis of differentiating adult oligodendrocytes during homeostasis and remyelination via the mitochondria-associated Fas-Casp8-Bid-axis
    Authors: KE Carlström, K Zhu, E Ewing, IE Krabbendam, RA Harris, AM Falcão, M Jagodic, G Castelo-Br, F Piehl
    Nat Commun, 2020;11(1):4071.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Cell Culture
  3. Reducing Th2 inflammation through neutralizing IL-4 antibody rescues myelination in IUGR rat brain
    Authors: AE Zanno, MA Romer, L Fox, T Golden, L Jaeckle-Sa, RA Simmons, JB Grinspan
    J Neurodev Disord, 2019;11(1):34.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Naked physically synthesized gold nanoparticles affect migration, mitochondrial activity, and proliferation of vascular smooth muscle cells
    Authors: HM Lo, MC Ma, JM Shieh, HL Chen, WB Wu
    Int J Nanomedicine, 2018;13(0):3163-3176.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Cantharidin attenuates the proliferation and migration of vascular smooth muscle cells through suppressing inflammatory response
    Authors: L Qiu, C Xu, H Jiang, W Li, S Tong, H Xia
    Biol. Pharm. Bull., 2018;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Bone marrow-derived mesenchymal stem cells inhibit vascular smooth muscle cell proliferation and neointimal hyperplasia after arterial injury in rats
    Authors: Y Iso, S Usui, M Toyoda, JL Spees, A Umezawa, H Suzuki
    Biochem Biophys Rep, 2018;16(0):79-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Dual Role of CREB in The Regulation of VSMC Proliferation: Mode of Activation Determines Pro- or Anti-Mitogenic Function
    Authors: C Hudson, TE Kimura, A Duggirala, GB Sala-Newby, AC Newby, M Bond
    Sci Rep, 2018;8(1):4904.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Wedelolactone, a plant coumarin, prevents vascular smooth muscle cell proliferation and injury-induced neointimal hyperplasia through Akt and AMPK signaling
    Authors: L Peng, X Huang, X Jin, Z Jing, L Yang, Y Zhou, J Ren, Y Zhao
    Exp. Gerontol., 2017;96(0):73-81.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Src-homology protein tyrosine phosphatase-1 agonist, SC-43, reduces liver fibrosis
    Authors: TH Su, CW Shiau, P Jao, NJ Yang, WT Tai, CJ Liu, TC Tseng, HC Yang, CH Liu, KW Huang, TC Hu, YJ Huang, YM Wu, LJ Chen, PJ Chen, DS Chen, KF Chen, JH Kao
    Sci Rep, 2017;7(1):1728.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Homer binds to Orai1 and TRPC channels in the neointima and regulates vascular smooth muscle cell migration and proliferation
    Authors: S Jia, M Rodriguez, AG Williams, JP Yuan
    Sci Rep, 2017;7(1):5075.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. 4-Hydroxybenzaldehyde accelerates acute wound healing through activation of focal adhesion signalling in keratinocytes
    Authors: CW Kang, YE Han, J Kim, JH Oh, YH Cho, EJ Lee
    Sci Rep, 2017;7(1):14192.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. Activation of Protein Kinase G (PKG) Reduces Neointimal Hyperplasia, Inhibits Platelet Aggregation, and Facilitates Re-endothelialization
    Sci Rep, 2016;6(0):36979.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Responses of adventitial CD34(+) vascular wall-resident stem/progenitor cells and medial smooth muscle cells to carotid injury in rats
    Authors: Jian Song
    Exp. Mol. Pathol., 2016;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Smooth Muscle Cell Pro-angiogenic Phenotype Induced by Cyclopentenyl Cytosine Promotes Endothelial Cell Proliferation and Migration
    Authors: Shi-You Chen
    J. Biol. Chem., 2016;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Epothilones Suppress Neointimal Thickening in the Rat Carotid Balloon-Injury Model by Inducing Vascular Smooth Muscle Cell Apoptosis through p53-Dependent Signaling Pathway
    PLoS ONE, 2016;11(5):e0155859.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  16. A 3D Poly(ethylene glycol)-based Tumor Angiogenesis Model to Study the Influence of Vascular Cells on Lung Tumor Cell Behavior
    Sci Rep, 2016;6(0):32726.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Sphingosine-1-phosphate mediates epidermal growth factor-induced muscle satellite cell activation.
    Authors: Nagata Y, Ohashi K, Wada E, Yuasa Y, Shiozuka M, Nonomura Y, Matsuda R
    Exp Cell Res, 2014;326(1):112-24.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. PSCs and GLP-1R: occurrence in normal pancreas, acute/chronic pancreatitis and effect of their activation by a GLP-1R agonist.
    Authors: Nakamura T, Ito T, Uchida M, Hijioka M, Igarashi H, Oono T, Kato M, Nakamura K, Suzuki K, Jensen R, Takayanagi R
    Lab Invest, 2014;94(1):63-78.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  19. TGFbeta-mediated suppression of CD248 in non-cancer cells via canonical Smad-dependent signaling pathways is uncoupled in cancer cells.
    Authors: Suresh Babu S, Valdez Y, Xu A, O'Byrne A, Calvo F, Lei V, Conway E
    BMC Cancer, 2014;14(0):113.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Systemic sclerosis immunoglobulin induces growth and a pro-fibrotic state in vascular smooth muscle cells through the epidermal growth factor receptor.
    Authors: Arts M, Baron M, Chokr N, Fritzler M, Servant M
    PLoS ONE, 2014;9(6):e100035.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  21. A lymphocyte-dependent mode of action for imatinib mesylate in experimental pulmonary hypertension.
    Authors: Ormiston M, Deng Y, Rundle N, Bendjelloul F, Tsoporis J, Parker T, Stewart D, Courtman D
    Am J Pathol, 2013;182(5):1541-51.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Endothelial dysfunction exacerbates renal interstitial fibrosis through enhancing fibroblast Smad3 linker phosphorylation in the mouse obstructed kidney.
    Authors: Sun Y, Qu X, Li X, Nikolic-Paterson D, Li J
    PLoS ONE, 2013;8(12):e84063.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  23. A naturally occurring carotenoid, lutein, reduces PDGF and H2O2 signaling and compromised migration in cultured vascular smooth muscle cells.
    Authors: Lo HM, Tsai YJ, Du WY, Tsou CJ, Wu WB
    J. Biomed. Sci., 2012;19(0):18.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  24. TGF beta1-Induced Baf60c Regulates both Smooth Muscle Cell Commitment and Quiescence.
    Authors: Sohni A, Mulas F, Ferrazzi F, Luttun A, Bellazzi R, Huylebroeck D, Ekker S, Verfaillie C
    PLoS ONE, 2012;7(10):e47629.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Endogenous angiogenesis inhibitor vasohibin1 exhibits broad-spectrum antilymphangiogenic activity and suppresses lymph node metastasis.
    Authors: Heishi T, Hosaka T, Suzuki Y, Miyashita H, Oike Y, Takahashi T, Nakamura T, Arioka S, Mitsuda Y, Takakura T, Hojo K, Matsumoto M, Yamauchi C, Ohta H, Sonoda H, Sato Y
    Am. J. Pathol., 2010;176(4):1950-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  26. Imatinib promotes osteoblast differentiation by inhibiting PDGFR signaling and inhibits osteoclastogenesis by both direct and stromal cell-dependent mechanisms.
    Authors: O'Sullivan S, Naot D, Callon K, Porteous F, Horne A, Wattie D, Watson M, Cornish J, Browett P, Grey A
    J. Bone Miner. Res., 2007;22(11):1679-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Lin(-)CD34(-) bone marrow cells from adult mice can differentiate into neural-like cells.
    Authors: Li Q, Hosaka N, Cui W, Wang X, Cui Y, Cui Y, Song C, Li Q, Ryu T, Fan T, Kawamoto K, Ikehara S
    Neurosci. Lett., 2006;408(1):51-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Ligand stimulation of CD155alpha inhibits cell adhesion and enhances cell migration in fibroblasts.
    Authors: Oda T, Ohka S, Nomoto A
    Biochem. Biophys. Res. Commun., 2004;319(4):1253-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Growth Factor Signaling Regulates Mechanical Nociception in Flies and Vertebrates.
    Authors: Lopez-Bellido R, Puig S, Huang P, Tsai C, Turner H, Galko M, Gutstein H
    J Neurosci, 0;39(30):6012-6030.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Rat PDGF-BB Protein

There are currently no reviews for this product. Be the first to review Recombinant Rat PDGF-BB Protein and earn rewards!

Have you used Recombinant Rat PDGF-BB Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review