Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the
alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production; temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.
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Key Product Details
- R&D Systems E. coli-derived Recombinant Rat PDGF-BB Protein (520-BB)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived rat PDGF-BB protein
Ser74-Thr182
Ser74-Thr182
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser74
Predicted Molecular Mass
25 kDa
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 1.5‑6 ng/mL.
The ED50 for this effect is 1.5‑6 ng/mL.
Reviewed Applications
Read 3 reviews rated 5 using 520-BB in the following applications:
Scientific Data Images for Recombinant Rat PDGF-BB Protein
Bioactivity of Rat PDGF-BB Protein
Recombinant rat PDGF-BB (520-BB) induces proliferation in the NR6R-3T3 mouse fibroblast cell line. The ED50 for this effect is 1.5-6 ng/mL.
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 520-BB
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 520-BB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: PDGF-BB
References
- Raines, E.W. et al. (1990) Platelet-derived growth factor in Handbook of Experimental Pharmacology, Peptide growth factors and their receptors, M.B. Sporn and A.B. Roberts, eds. p. 173.
Long Name
Platelet-derived Growth Factor BB
Alternate Names
PDGFBB
Gene Symbol
PDGFB
UniProt
Additional PDGF-BB Products
Product Documents for Recombinant Rat PDGF-BB Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Rat PDGF-BB Protein
For research use only
Related Research Areas
Citations for Recombinant Rat PDGF-BB Protein
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Customer Reviews for Recombinant Rat PDGF-BB Protein (3)
5 out of 5
3 Customer Ratings
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Application: Cell ProliferationVerified Customer | Posted 05/20/2023PDGF-BB Protein promote cell differentiation
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 03/01/2022
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 08/25/202110 ng/ml PDGF were added to ipsc cell culture medium.
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