Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
Proteome Profiler Rat XL Cytokine Array
R&D Systems | Catalog # ARY030
Key Product Details
Species
Product Summary for Proteome Profiler Rat XL Cytokine Array
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine.
Key Benefits
- Detects 79 rat cytokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Rat XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample
Kit Contents
- Rectangular 4-Well Multi-dish
- 4 Rat XL Cytokine Array nitrocellulose membranes spotted with 79 different antibodies to rat cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Rat XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
| Simultaneously detect the levels of these proteins in a single sample. | ||
|---|---|---|
| Adiponectin | Galectin-3 | LIX |
| CCL2/JE/MCP-1 | G-CSF | MAG/Siglec-4a |
| CCL3/CCL4/MIP-1α/β | GDF-15 | MMP-2 |
| CCL5/RANTES | GM-CSF | MMP-3 |
| CCL11/Eotaxin | Hepassocin | MMP-9 |
| CCL17/TARC | HGF | Neprilysin/CD10 |
| CCL20/MIP-3α | ICAM-1/CD54 | NOV/CCN3 |
| CCL21/6Ckine | IFN-γ | NT-3 |
| CCL22/MDC | IGF-I | NT-4 |
| Clusterin | IGFBP-2 | Osteopontin (OPN) |
| CNTF | IGFBP-3 | Osteoprotegerin/TNFRSF11B |
| CX3CL1/Fractalkine | IGFBP-5 | PDGF-BB |
| CXCL2/GROβ/MIP-2/CINC-3 | IGFBP-6 | Pref-1/DLK1/FA1 |
| CXCL7/Thymus Chemokine-1 | IL-1α/IL-1F1 | Prolactin |
| Cyr61/CCN1 | IL-1β/IL-1F2 | RAGE |
| Cystatin C | IL-1ra/IL-1F3 | RBP4 |
| DPPIV/CD26 | IL-2 | Resistin |
| EGF | IL-3 | RGM-A |
| EG-VEGF | IL-4 | SCF |
| Endostatin | IL-6 | Serpin E1/PAI-1 |
| Fetuin A | IL-13 | TIM-1/KIM-1/HAVCR |
| FGF acidic | IL-17A | TNF-α |
| FGF-7 | IL-22 | TWEAK/TNFSF12 |
| FGF-21 | Jagged 1 | VCAM-1/CD106 |
| Fibulin 3 | LIF | VEGF |
| Flt-3 Ligand | Lipocalin-2/NGAL | WISP-1/CCN |
| Galectin-1 | ||
Assays for Analytes represented in the Rat XL Cytokine Array
| Analyte | Quantikine® ELISA Kits | DuoSet® ELISA Development System |
|---|---|---|
| Adiponectin | RRP300 | DY3100-05 |
| CCL2/JE/MCP-1 | MJE00 | |
| CCL3/CCL4/MIP-1α/β | ||
| CCL5/RANTES | ||
| CCL11/Eotaxin | ||
| CCL17/TARC | ||
| CCL20/MIP-3α | DY540 | |
| CCL21/6Ckine | ||
| CCL22/MDC | ||
| Clusterin | ||
| CNTF | DY557 | |
| CX3CL1/Fractalkine | DY537 | |
| CXCL2/GROβ/MIP-2/CINC-3 | RCN300 | DY525 |
| CXCL7/Thymus Chemokine-1 | DY1116 | |
| Cyr61/CCN1 | ||
| Cystatin C | ||
| DPPIV/CD26 | ||
| EGF | DY3214 | |
| EG-VEGF | ||
| Endostatin | ||
| Fetuin A | ||
| FGF acidic | ||
| FGF-7 | ||
| FGF-21 | ||
| Fibulin 3 | ||
| Flt-3 Ligand | ||
| Galectin-1 | ||
| Galectin-3 | ||
| G-CSF | ||
| GDF-15 | MGD150 | |
| GM-CSF | DY518 | |
| Hepassocin | ||
| HGF | ||
| ICAM-1/CD54 | RIC100 | DY583 |
| IFN-γ | PRIF00 | DY585 |
| IGF-I | ||
| IGFBP-2 | ||
| IGFBP-3 | ||
| IGFBP-5 | ||
| IGFBP-6 | ||
| IL-1α/IL-1F1 | RRA00 | DY500 |
| IL-1β/IL-1F2 | PRLB00 | DY501 |
| IL-1ra/IL-1F3 | ||
| IL-2 | PR2000 | DY502 |
| IL-3 | ||
| IL-4 | R4000 | DY504 |
| IL-6 | PR6000B | DY506 |
| IL-13 | ||
| IL-17A | ||
| IL-22 | ||
| Jagged 1 | DY599 | |
| LIF | ||
| Lipocalin-2/NGAL | DY3508 | |
| LIX | DY543 | |
| MAG/Siglec-4a | DY538 | |
| MMP-2 | ||
| MMP-3 | ||
| MMP-9 | RMP900 | DY8174-05 |
| Neprilysin/CD10 | ||
| NOV/CCN3 | ||
| NT-3 | ||
| NT-4 | ||
| Osteopontin (OPN) | ||
| Osteoprotegerin/TNFRSF11B | ||
| PDGF-BB | MBB00 | DY8464-05 |
| Pref-1/DLK1/FA1 | ||
| Prolactin | ||
| RAGE | DY1616 | |
| RBP4 | ||
| Resistin | ||
| RGM-A | ||
| SCF | ||
| Serpin E1/PAI-1 | ||
| TIM-1/KIM-1/HAVCR | ||
| TNF-α | PRTA00 | DY510 |
| TWEAK/TNFSF12 | ||
| VCAM-1/CD106 | ||
| VEGF | RRV00 | DY564 |
| WISP-1/CCN |
Scientific Data Images for Proteome Profiler Rat XL Cytokine Array
The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 1A: NR8383 normal rat lung cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure).
The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 1B: IEC-18 rat intestinal epithelial cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure).
The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 2A: Lysates from IEC-18 rat intestinal epithelial cells were left untreated or treated with 100 ng/mL PMA for 24 hours (200 μg lysate, 5 minute exposure.
The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. Figure 2B: Lysates from NR8383 normal rat lung cells were left untreated or treated with 5 μg/mL zymosan for 24 hours (200 μg lysate, 5 minute exposure).Formulation, Preparation, and Storage
Shipping
Storage
Background: Multiplex Cytokine Assay Kits
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Proteome Profiler Rat XL Cytokine Array
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Proteome Profiler Rat XL Cytokine Array
For research use only
Citations for Proteome Profiler Rat XL Cytokine Array
Powered by Bioz
Customer Reviews for Proteome Profiler Rat XL Cytokine Array (2)
Have you used Proteome Profiler Rat XL Cytokine Array?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
-
Verified Customer | Posted 04/03/2018
-
Verified Customer | Posted 04/01/2018
There are no reviews that match your criteria.
FAQs
No product specific FAQs exist for this product.
View all FAQs for Proteome Profiler Antibody Arrays