A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine.
Detects 79 rat cytokines simultaneously
Requires no specialized equipment
Compatible with LI-COR and chemiluminescence detection
The Proteome Profiler Rat XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
Uses pre-mixed differentiation cocktails to optimally drive reproducible differentiation of pluripotent stem cells into hepatocyte-like cells.
Yields a highly enriched (> 70% purity) and healthy population of hepatocyte-like cells.
Produces hepatocyte-like cells that express Asialoglycoprotein Receptor 1, alpha-Fetoprotein, Hepatocyte Nuclear Factor 4 alpha, CEBP-alpha, Cytokeratin 18, and Serpin A1.
Produces hepatocyte-like cells that function similarly to human liver cells, including lipid and glycogen storage, urea secretion, albumin secretion, and functional cytochrome p450 activity.
Can be part of small molecule and drug toxicity screening workflows.
This kit contains the following azide-free, fluorochrome-conjugated antibodies to verify human stem cell pluripotency:
Rectangular 4-Well Multi-dish
4 Rat XL Cytokine Array nitrocellulose membranes spotted with 79 different antibodies to rat cytokines
Array Buffer 4
Array Buffer 6
Chemi Reagent 1
Chemi Reagent 2
Detection Antibody Cocktail, Rat XL Cytokine Array
Transparency Overlay Template
Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Simultaneously detect the levels of these proteins in a single sample.
CCL3/CCL4/MIP-1 alpha / beta
CXCL2/GRO beta /MIP-2/CINC-3
IL-1 alpha /IL-1F1
IL-1 beta /IL-1F2
Assays for Analytes represented in the Rat XL Cytokine Array
The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
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