Proteome Profiler Rat XL Cytokine Array ARY030: R&D Systems
Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine.

Key Benefits

  • Detects 79 rat cytokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection
 

 

Principle of the Assay

The Proteome Profiler Rat XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

  • Uses pre-mixed differentiation cocktails to optimally drive reproducible differentiation of pluripotent stem cells into hepatocyte-like cells.
  • Yields a highly enriched (> 70% purity) and healthy population of hepatocyte-like cells.
  • Produces hepatocyte-like cells that express Asialoglycoprotein Receptor 1, alpha-Fetoprotein, Hepatocyte Nuclear Factor 4 alpha, CEBP-alpha, Cytokeratin 18, and Serpin A1.
  • Produces hepatocyte-like cells that function similarly to human liver cells, including lipid and glycogen storage, urea secretion, albumin secretion, and functional cytochrome p450 activity.
  • Can be part of small molecule and drug toxicity screening workflows.

 

Kit Contents

This kit contains the following azide-free, fluorochrome-conjugated antibodies to verify human stem cell pluripotency:

  • Rectangular 4-Well Multi-dish
  • 4 Rat XL Cytokine Array nitrocellulose membranes spotted with 79 different antibodies to rat cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Rat XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Simultaneously detect the levels of these proteins in a single sample.

Adiponectin Galectin-3 LIX
CCL2/JE/MCP-1 G-CSF MAG/Siglec-4a
CCL3/CCL4/MIP-1 alpha / beta GDF-15 MMP-2
CCL5/RANTES GM-CSF MMP-3
CCL11/Eotaxin Hepassocin MMP-9
CCL17/TARC HGF Neprilysin/CD10
CCL20/MIP-3 alpha ICAM-1/CD54 NOV/CCN3
CCL21/6Ckine IFN-gamma NT-3
CCL22/MDC IGF-I NT-4
Clusterin IGFBP-2 Osteopontin (OPN)
CNTF IGFBP-3 Osteoprotegerin/TNFRSF11B
CX3CL1/Fractalkine IGFBP-5 PDGF-BB
CXCL2/GRO beta /MIP-2/CINC-3 IGFBP-6 Pref-1/DLK1/FA1
CXCL7/Thymus Chemokine-1  IL-1 alpha /IL-1F1 Prolactin
Cyr61/CCN1 IL-1 beta /IL-1F2 RAGE
Cystatin C  IL-1ra/IL-1F3 RBP4
DPPIV/CD26 IL-2 Resistin
EGF IL-3 RGM-A
EG-VEGF IL-4 SCF
Endostatin IL-6 Serpin E1/PAI-1
Fetuin A IL-13 TIM-1/KIM-1/HAVCR
FGF acidic IL-17A TNF-alpha
FGF-7 IL-22 TWEAK/TNFSF12
FGF-21 Jagged 1 VCAM-1/CD106
Fibulin 3 LIF VEGF
Flt-3 Ligand Lipocalin-2/NGAL WISP-1/CCN
Galectin-1    

Assays for Analytes represented in the Rat XL Cytokine Array

Analyte Q-kit DuoSet
Adiponectin RRP300 DY3100-05
CCL2/JE/MCP-1 MJE00  
CCL3/CCL4/MIP-1 alpha / beta    
CCL5/RANTES    
CCL11/Eotaxin    
CCL17/TARC    
CCL20/MIP-3 alpha   DY540
CCL21/6Ckine    
CCL22/MDC    
Clusterin    
CNTF   DY557
CX3CL1/Fractalkine   DY537
CXCL2/GRO beta /MIP-2/CINC-3 RCN300 DY525
CXCL7/Thymus Chemokine-1   DY1116
Cyr61/CCN1    
Cystatin C    
DPPIV/CD26    
EGF   DY3214
EG-VEGF    
Endostatin    
Fetuin A    
FGF acidic    
FGF-7    
FGF-21    
Fibulin 3    
Flt-3 Ligand    
Galectin-1    
Galectin-3    
G-CSF    
GDF-15 MGD150  
GM-CSF   DY518
Hepassocin    
HGF    
ICAM-1/CD54 RIC100 DY583
IFN-gamma PRIF00 DY585
IGF-I    
IGFBP-2    
IGFBP-3    
IGFBP-5    
IGFBP-6    
 IL-1 alpha /IL-1F1 RRA00 DY500
IL-1 beta /IL-1F2 PRLB00 DY501
 IL-1ra/IL-1F3    
IL-2 PR2000 DY502
IL-3    
IL-4 R4000 DY504
IL-6 PR6000B DY506
IL-13    
IL-17A    
IL-22    
Jagged 1   DY599
LIF    
Lipocalin-2/NGAL   DY3508
LIX   DY543
MAG/Siglec-4a   DY538
MMP-2    
MMP-3    
MMP-9 RMP900 DY8174-05
Neprilysin/CD10    
NOV/CCN3    
NT-3    
NT-4    
Osteopontin (OPN)    
Osteoprotegerin/TNFRSF11B    
PDGF-BB MBB00 DY8464-05
Pref-1/DLK1/FA1    
Prolactin    
RAGE   DY1616
RBP4    
Resistin    
RGM-A    
SCF    
Serpin E1/PAI-1    
TIM-1/KIM-1/HAVCR    
TNF-alpha PRTA00 DY510
TWEAK/TNFSF12    
VCAM-1/CD106    
VEGF RRV00 DY564
WISP-1/CCN    

 

Data Examples

The Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

Proteome Profiler XL Cytokine Array Kit ARY030 Figure 1A.
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Figure 1A: NR8383 normal rat lung cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure).

Proteome Profiler XL Cytokine Array Kit ARY030 Figure 1A.
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Figure 1B: IEC-18 rat intestinal epithelial cells were left untreated or treated with 1 μg/mL LPS for 24 hours (500 μL of cell culture supernate, 5 minute exposure).

Proteome Profiler XL Cytokine Array Kit ARY030 Figure 2A.
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Figure 2A: Lysates from IEC-18 rat intestinal epithelial cells were left untreated or treated with 100 ng/mL PMA for 24 hours (200 μg lysate, 5 minute exposure.

Proteome Profiler XL Cytokine Array Kit ARY030 Figure 2B.
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Figure 2B: Lysates from NR8383 normal rat lung cells were left untreated or treated with 5 μg/mL zymosan for 24 hours (200 μg lysate, 5 minute exposure).

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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