ROCK2 Antibody (1E12) - Azide and BSA Free

Name: ROCK2 Antibody (1E12) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00009475-M02
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # H00009475-M02

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 1E12

Format

Azide and BSA Free

View all ROCK2 Primary Antibodies »

Product Specifications

Immunogen

ROCK2 (NP_004841, 1279 a.a. ~ 1388 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Reactivity Notes

Other species not tested.

Specificity

ROCK2 (1E12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for ROCK2 Antibody (1E12) - Azide and BSA Free

Western Blot: ROCK2 Antibody (1E12) [H00009475-M02]

Western Blot: ROCK2 Antibody (1E12) [H00009475-M02] - ROCK2 monoclonal antibody (M02), clone 1E12 Analysis of ROCK2 expression in Hela S3 NE.

Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (1E12) [H00009475-M02]

Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (1E12) [H00009475-M02] - Analysis of monoclonal antibody to ROCK2 on HeLa cell. Antibody concentration 30 ug/ml.

Immunohistochemistry-Paraffin: ROCK2 Antibody (1E12) [H00009475-M02]

Immunohistochemistry-Paraffin: ROCK2 Antibody (1E12) [H00009475-M02] - Analysis of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1.5 ug/ml.

Sandwich ELISA: ROCK2 Antibody (1E12) [H00009475-M02] - Detection limit for recombinant GST tagged ROCK2 is 0.3 ng/ml as a capture antibody.

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Applications for ROCK2 Antibody (1E12) - Azide and BSA Free

Application

Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500

Application Notes

Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, ELISA and immunohistochemistry (paraffin).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ROCK2

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Alternate Names

ROCK-II, ROKalpha

Entrez Gene IDs

9475 (Human)

Gene Symbol

ROCK2

OMIM

604002 (Human)

UniProt

NP_004841 (Human)

Additional ROCK2 Products

Product Documents for ROCK2 Antibody (1E12) - Azide and BSA Free

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ROCK2 Antibody (1E12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.