ROCK2 Antibody - BSA Free

Name: ROCK2 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-20199
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-20199

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all ROCK2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary.

Localization

Cytoplasm (By similarity); Cell membrane; Peripheral membrane protein (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit ROCK2 Antibody - BSA Free (NBP2-20199) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ROCK2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody [NBP2-20199]

Immunocytochemistry/Immunofluorescence: ROCK2 Antibody [NBP2-20199] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199]

Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199] - Human esophagous cancer, using ROCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: ROCK2 Antibody [NBP2-20199]

Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: ROCK2 Antibody [NBP2-20199]

Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 5% SDS PAGE gel, diluted at 1:500.

Western Blot: ROCK2 Antibody [NBP2-20199]

Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (50 ug of whole cell lysate) A: Rat brain, 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: ROCK2 Antibody [NBP2-20199] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [N3C1], Internal (NBP2-20199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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Applications for ROCK2 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ROCK2

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq]

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Alternate Names

ROCK-II, ROKalpha

Gene Symbol

ROCK2

UniProt

O75116

Additional ROCK2 Products

Product Documents for ROCK2 Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for ROCK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.