SENP6 Antibody (4B7) - Azide and BSA Free

Novus Biologicals | Catalog # H00026054-M01

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B7

Format

Azide and BSA Free
View all SENP6 Primary Antibodies »

Product Specifications

Immunogen

SENP6 (NP_056386, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAGKSGGSAGEITFLEALARSESKRDGGFKNNWSFDHEEESEGDTDKDGTNLLSVDEDEDSETSKGKKLNRRSEIVANSSGEFILKTYVRRNKSESFKTLKGNPIGLNM

Specificity

SENP6 - SUMO1/sentrin specific protease 6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SENP6 Antibody (4B7) - Azide and BSA Free

Western Blot: SENP6 Antibody (4B7) [H00026054-M01]

Western Blot: SENP6 Antibody (4B7) [H00026054-M01]

Western Blot: SENP6 Antibody (4B7) [H00026054-M01] - SENP6 monoclonal antibody (M01), clone 4B7 Analysis of SENP6 expression in IMR-32.
Immunohistochemistry-Paraffin: SENP6 Antibody (4B7) [H00026054-M01]

Immunohistochemistry-Paraffin: SENP6 Antibody (4B7) [H00026054-M01]

Immunohistochemistry-Paraffin: SENP6 Antibody (4B7) [H00026054-M01] - Analysis of monoclonal antibody to SENP6 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Flow Cytometry: SENP6 Antibody (4B7) [H00026054-M01]

Flow Cytometry: SENP6 Antibody (4B7) [H00026054-M01]

SENP6-Antibody-4B7-Flow-Cytometry-H00026054-M01-img0009.jpg
Flow Cytometry: SENP6 Antibody (4B7) [H00026054-M01]

Flow Cytometry: SENP6 Antibody (4B7) [H00026054-M01]

Flow Cytometry: SENP6 Antibody (4B7) [H00026054-M01] - Staining with senp6 antibody and negative controls, acquisition by FACS. Image from verified customer review.
Flow Cytometry: SENP6 Antibody (4B7) [H00026054-M01]

Flow Cytometry: SENP6 Antibody (4B7) [H00026054-M01]

SENP6-Antibody-4B7-Flow-Cytometry-H00026054-M01-img0010.jpg
Sandwich ELISA: SENP6 Antibody (4B7) [H00026054-M01] - Detection limit for recombinant GST tagged SENP6 is approximately 0.03ng/ml as a capture antibody.

Applications for SENP6 Antibody (4B7) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Flow data from customer review. ICC data from customer review.

Reviewed Applications

Read 1 review rated 4 using H00026054-M01 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SENP6

Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1; MIM 601912), are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins (Kim et al., 2000 [PubMed 10799485]).[supplied by OMIM]

Alternate Names

EC 3.4.22.-, KIAA0797FLJ11887, Sentrin/SUMO-specific protease SENP6, sentrin-specific protease 6, SSP1KIAA0389, SUMO1/sentrin specific peptidase 6,2810017C20Rik, SUMO1/sentrin specific protease 6, SUMO-1-specific protease 1, SUSP1FLJ11355

Entrez Gene IDs

26054 (Human)

Gene Symbol

SENP6

OMIM

605003 (Human)

UniProt

NP_056386 (Human)

Additional SENP6 Products

Product Documents for SENP6 Antibody (4B7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SENP6 Antibody (4B7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SENP6 Antibody (4B7) - Azide and BSA Free

Customer Reviews for SENP6 Antibody (4B7) - Azide and BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used SENP6 Antibody (4B7) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human Cell lines
    Species: Human
    Verified Customer | Posted 04/25/2014
    staning with senp6 antibody and negative controls, acquisition by FACS
    SENP6 Antibody (4B7) - Azide and BSA Free H00026054-M01

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies