SHC1 Antibody

Name: SHC1 Antibody
Brand: Novus Biologicals
SKU: NBP3-23392
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23392

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Rabbit

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all SHC1 Primary Antibodies »

Product Specifications

Immunogen

A recombinant protein that included amino acid residues within the C-terminal region of human SHC1. This sequence has high homology with similar regions in rat and mouse SHC1. [UniProt# P29353]

Specificity

The antibody detects 46/52/66 kDa bands corresponding to the molecular weight of SHC1 variants on SDS-PAGE immunoblots of A431 cells. It also detects SHC1 in human Jurkat, HeLa, and SKN-SH cells, as well as rat brain tissue and rabbit spleen fibroblasts.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for SHC1 Antibody

Western Blot: SHC1 Antibody [NBP3-23392] -

Western Blot: SHC1 Antibody [NBP3-23392] - Western blot analysis of SHC1 expression in A431 cell lysate (lanes 1, 2, 3, & 4). The blots were probed with rabbit polyclonal anti-SHC1 (NBP3-23392) at 1:1000 (lane 1) or 1:4000 (lane 2) and mouse monoclonal SHC1 (C-terminal region) at 1:1000 (lane 3) or 1:4000 (lane 4).

Immunocytochemistry/Immunofluorescence: SHC1 Antibody [NBP3-23392] -

Immunocytochemistry/Immunofluorescence: SHC1 Antibody [NBP3-23392] - Immunocytochemical labeling of SHC1 in paraformaldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal (top) and rabbit polyclonal (bottom NBP3-23392) SHC1 antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Applications for SHC1 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SHC1

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Gene Symbol

SHC1

Additional SHC1 Products

Product Documents for SHC1 Antibody

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SHC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.