hh Sheep IgG HRP-conjugated Antibody HAF016: R&D Systems

Sheep IgG HRP-conjugated Antibody

(14 citations)
(4 Reviews)
  
  • Species Reactivity
    Sheep
  • Specificity
    Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.
  • Source
    Polyclonal Donkey IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Sheep IgG
  • Formulation
    Sodium Phosphate and Proclin 300.
  • Label
    HRP (Horseradish Peroxidase)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Use at a 1:1000 dilution.
  • Simple Western
    Use at a 1:50 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SheepIgG Primary Antibody by Western Blot Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF7565)using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody(Catalog # HAF016).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Detection of Sheep IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody
(Catalog #AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease
    Authors: JM Van Kampen, DG Kay
    PLoS ONE, 2017;12(8):e0182896.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Rnd3-induced cell rounding requires interaction with Plexin-B2
    J Cell Sci, 2016;0(0):.
    Species: Primate - Chlorocebus sabaeus (Green Monkey)
    Sample Type: Cell Lysates
    Application: WB
  3. Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function
    J Neurosci, 2016;36(35):9201-16.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Targeting tachykinin receptors in neuroblastoma
    Oncotarget, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies.
    Authors: Althoff K, Beckers A, Bell E, Nortmeyer M, Thor T, Sprussel A, Lindner S, De Preter K, Florin A, Heukamp L, Klein-Hitpass L, Astrahantseff K, Kumps C, Speleman F, Eggert A, Westermann F, Schramm A, Schulte J
    Oncogene, 2015;34(26):3357-68.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  6. A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment.
    Authors: Nyegaard M, Rendtorff N, Nielsen M, Corydon T, Demontis D, Starnawska A, Hedemand A, Buniello A, Niola F, Overgaard M, Leal S, Ahmad W, Wikman F, Petersen K, Cruger D, Oostrik J, Kremer H, Tommerup N, Frodin M, Steel K, Tranebjaerg L, Borglum A
    PLoS Genet, 2015;11(7):e1005386.
    Species: Human
    Sample Type: Cell Lysates
  7. Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis.
    Authors: Bose C, Megyesi J, Shah S, Hiatt K, Hall K, Karaduta O, Swaminathan S
    PLoS ONE, 2015;10(8):e0136563.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Bacterial delivery of Staphylococcus aureus alpha-hemolysin causes regression and necrosis in murine tumors.
    Authors: St Jean A, Swofford C, Panteli J, Brentzel Z, Forbes N
    Mol Ther, 2014;22(7):1266-74.
    Species: Bacteria - E. Coli
    Sample Type: Cell Lysates
    Application: WB
  9. SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/beta-catenin signaling.
    Authors: Thu K, Radulovich N, Becker-Santos D, Pikor L, Pusic A, Lockwood W, Lam W, Tsao M
    Oncogene, 2014;33(3):279-88.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Antibodies that detect O-linked beta-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins.
    Authors: Tashima Y, Stanley P
    J Biol Chem, 2014;289(16):11132-42.
  11. ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
    Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
    Exp Cell Res, 2014;323(2):263-75.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions.
    Authors: Nakamura R, Nakamoto C, Obama H
    J. Biol. Chem., 2012;287(5):3282-91.
    Species: Sheep
    Sample Type: Antibody
    Application: WB
  13. Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription.
    Authors: Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A
    PLoS ONE, 2012;7(4):e34703.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  14. Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
    Authors: Bekku Y, Oohashi T,
    Arch. Histol. Cytol., 2010;73(2):95-102.
    Species: Mouse
    Sample Type: Tissue Homogenates
Expand to show all 14 Citations

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
View all Antibody FAQs
Secondary Antibodies
Description Application Cat# Citations Images  

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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Average Rating: 4.8 (Based on 4 reviews)

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Read what people are saying who have used Sheep IgG HRP-conjugated Antibody.

We have 3 reviews tested in 1 species: Human.
We have 4 reviews tested in 2 applications: Western Blot, ELISA.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Sheep IgG HRP-conjugated Antibody HAF016
Enlarge
Very Good
 WB Human Anonymous 11/15/2017
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Image Details
Western Blot Sheep IgG HRP-conjugated Antibody HAF016
Western Blot: Sheep IgG HRP-conjugated Antibody [HAF016]

Summary

ApplicationWestern Blot
Sample TestedCalu-6 human lung carcinoma cell line,H1299 human lung carcinoma cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsPrimary Ab = Anti SSBP1 Polyclonal Sheep IgG AF6588 (1:200)Secondary Ab = 1:1000
  Excellent
 ELISA Human Anonymous 10/23/2017
Show

Summary

ApplicationELISA
Sample TestedHuman recombinant antibody
SpeciesHuman
Western Blot Sheep IgG HRP-conjugated Antibody HAF016
Enlarge
Excellent
 WB Human Anonymous 06/13/2017
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Image Details
Western Blot Sheep IgG HRP-conjugated Antibody HAF016
Western Blot: Sheep IgG HRP-conjugated Antibody [HAF016]

Summary

ApplicationWestern Blot
Sample TestedHASMC human aortic smooth muscle cells
SpeciesHuman

Other Experimental Details

Other Experimental Detailsdil: 1:6000 in 5% skim milk powder and TBST.
  Excellent
 WB Anonymous 07/27/2016
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Summary

ApplicationWestern Blot
Sample TestedTissue sections

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