Key Product Details

Species Reactivity

Sheep

Applications

Immunohistochemistry, Western Blot, Simple Western

Label

HRP

Antibody Source

Polyclonal Donkey IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Sheep IgG

Specificity

Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Sheep IgG Horseradish Peroxidase-conjugated Antibody

Detection of Sheep IgG Primary Antibody by Western Blot

Detection of Sheep IgG Primary Antibody by Western Blot

Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1 Antigen Affinity-purified Polyclonal Antibody (AF7565) using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Sheep IgG Primary Antibody by Simple WesternTM.

Detection of Sheep IgG Primary Antibody by Simple WesternTM.

Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody (AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of AIF in Human Kidney

Detection of AIF in human kidney using 3 ug/mL of Sheep anti-human AIF primary antibody (AF5824) followed by 30 min incubation with Donkey anti Sheep HRP conjugated secondary antibodies (Catalog # HAF016) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.
Sheep IgG Horseradish Peroxidase-conjugated Antibody

Sheep IgG Horseradish Peroxidase-conjugated Antibody

BHS and DS serum antibodies to Mycoplasma ovipneumoniae pre-inoculation and 28-days post-inoculation. The ladder and associated kDa values are in the first sample lane. Thereafter, the pre-sample is in the first lane for each animal in the inoculation study and is followed by the 28-day post-inoculation (dPI) serum sample. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39770861), licensed under a CC-BY license. Not internally tested by R&D Systems.
Sheep IgG Horseradish Peroxidase-conjugated Antibody

Sheep IgG Horseradish Peroxidase-conjugated Antibody

Activity of Homo-BacPROTACs in cell-free degradation assays.a Degradation curves of ClpC1-NTD in the cell-free degradation assay (quantified by capillary Western (WES)) induced by Homo-BacPROTAC 8 (UdSBI-0545) compared to its enantiomer 8a (UdSBI-0966), matching monomer 5 and dCymC (3 independent experiments done in triplicates). b WES visualization of ClpC1-NTD degradation after titration of Homo-BacPROTACs, developed with anti-His antibody recognizing His6-tagged ClpC1-NTD and processed, His4-tagged ClpP1P2. Concentration-dependent degradation of ClpC1-NTD can be observed for 8 (UdSBI-0545) (lanes 10–13) but not for 8a (UdSBI-0966) (lanes 2–8). c Analogous to a, except that exit vector 7-based Homo-BacPROTAC 12 (UdSBI-4377), enantiomer 12a (UdSBI-0117) and monomer 10 were used. d WES-derived gel picture visualizing ClpC1-NTD degradation (lane 2–5) from representative experiment summarized in c. e SYPROTM Ruby-stained SDS-PAGE gel from exemplary cell-free degradation assay depicting efficient degradation (lane 3,4) of ClpC1-NTD by Homo-BacPROTAC 8 (UdSBI-0545), while full length ClpC1 is not significantly degraded. Green vertical lines indicate the DC50 (for 8,12). Error bars represent mean ± SD of n = 3 independent experiments in triplicates. The actual mean DC50 values for all cell-free degradation experiments conducted for this study are summarized in Supplementary Table 8. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-46218-7), licensed under a CC-BY license. Not internally tested by R&D Systems.
Sheep IgG Horseradish Peroxidase-conjugated Antibody

Sheep IgG Horseradish Peroxidase-conjugated Antibody

Activity of Homo-BacPROTACs in cell-free degradation assays.a Degradation curves of ClpC1-NTD in the cell-free degradation assay (quantified by capillary Western (WES)) induced by Homo-BacPROTAC 8 (UdSBI-0545) compared to its enantiomer 8a (UdSBI-0966), matching monomer 5 and dCymC (3 independent experiments done in triplicates). b WES visualization of ClpC1-NTD degradation after titration of Homo-BacPROTACs, developed with anti-His antibody recognizing His6-tagged ClpC1-NTD and processed, His4-tagged ClpP1P2. Concentration-dependent degradation of ClpC1-NTD can be observed for 8 (UdSBI-0545) (lanes 10–13) but not for 8a (UdSBI-0966) (lanes 2–8). c Analogous to a, except that exit vector 7-based Homo-BacPROTAC 12 (UdSBI-4377), enantiomer 12a (UdSBI-0117) and monomer 10 were used. d WES-derived gel picture visualizing ClpC1-NTD degradation (lane 2–5) from representative experiment summarized in c. e SYPROTM Ruby-stained SDS-PAGE gel from exemplary cell-free degradation assay depicting efficient degradation (lane 3,4) of ClpC1-NTD by Homo-BacPROTAC 8 (UdSBI-0545), while full length ClpC1 is not significantly degraded. Green vertical lines indicate the DC50 (for 8,12). Error bars represent mean ± SD of n = 3 independent experiments in triplicates. The actual mean DC50 values for all cell-free degradation experiments conducted for this study are summarized in Supplementary Table 8. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-46218-7), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Sheep IgG Horseradish Peroxidase-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Use at a 1:20 dilution 

Simple Western

Use at a 1:50 dilution.

Western Blot

Use at a 1:1000 dilution.

Reviewed Applications

Read 14 reviews rated 4.9 using HAF016 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Sodium Phosphate and Proclin 300.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Sheep IgG Horseradish Peroxidase-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Sheep IgG Horseradish Peroxidase-conjugated Antibody

For research use only

Citations for Sheep IgG Horseradish Peroxidase-conjugated Antibody

Customer Reviews for Sheep IgG Horseradish Peroxidase-conjugated Antibody (14)

4.9 out of 5
14 Customer Ratings
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Customer Images

Showing  1 - 5 of 14 reviews Showing All
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  • Sheep IgG Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human serum
    Species: Human
    Verified Customer | Posted 08/12/2023
    plate reading
    ELISA for human serum
    Sheep IgG Horseradish Peroxidase-conjugated Antibody HAF016
  • Sheep IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 05/27/2022
    Sheep IgG Horseradish Peroxidase-conjugated Antibody HAF016
  • Sheep IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Aortic smooth muscle cells
    Species: Human
    Verified Customer | Posted 12/05/2021
    Sheep IgG Horseradish Peroxidase-conjugated Antibody HAF016
  • Sheep IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 10/11/2021
    Dilution: 1:1000 in skim milk in TBST (Incubation: 1hr at room temperature)
    Sheep IgG Horseradish Peroxidase-conjugated Antibody HAF016
  • Sheep IgG HRP-conjugated Antibody
    Name: Raminder Singh
    Application: Western Blot
    Sample Tested: C2BBe1 cell lysate
    Species: Human
    Verified Customer | Posted 08/16/2021
    C2BBe1 cell cysate was probed for CEACAM 7 with Sheep Anti-Human CEACAM 7 Antibody (Catalog # AF4478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016)
    Sheep IgG Horseradish Peroxidase-conjugated Antibody HAF016
  • Sheep IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: PC-3 human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 07/30/2021
    Sheep IgG Horseradish Peroxidase-conjugated Antibody HAF016
  • Sheep IgG HRP-conjugated Antibody
    Name: Takuya Shuo
    Application: Western Blot
    Sample Tested: iPS cells-derived neuron and Recombinant protein
    Species: Human
    Verified Customer | Posted 06/23/2020
    Sheep IgG Horseradish Peroxidase-conjugated Antibody HAF016
  • Sheep IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 05/20/2019
  • Sheep IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HepG2 human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 07/15/2018
    Western blotting detection of CD47 using sheep polyclonal antibody (AF4670).
    Sheep IgG Horseradish Peroxidase-conjugated Antibody HAF016
  • Sheep IgG HRP-conjugated Antibody
    Name: Danlee Enzler
    Application: ELISA
    Sample Tested: Serum
    Species: Sheep
    Verified Customer | Posted 05/01/2018
  • Sheep IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Calu-6 human lung carcinoma cell line and H1299 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 11/15/2017
    Primary Ab = Anti SSBP1 Polyclonal Sheep IgG AF6588 (1:200) Secondary Ab = 1:1000
    Sheep IgG Horseradish Peroxidase-conjugated Antibody HAF016
  • Sheep IgG HRP-conjugated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Human recombinant antibody
    Species: Human
    Verified Customer | Posted 10/23/2017
  • Sheep IgG HRP-conjugated Antibody
    Name: Jagadeesh Janjanam
    Application: Western Blot
    Sample Tested: HASMC human aortic smooth muscle cells
    Species: Human
    Verified Customer | Posted 06/13/2017
    dil: 1:6000 in 5% skim milk powder and TBST.
  • Sheep IgG Horseradish Peroxidase-conjugated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Tissue sections
    Species: Sheep
    Verified Customer | Posted 07/27/2016

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Protocols

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FAQs for Sheep IgG Horseradish Peroxidase-conjugated Antibody

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  • Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?

    A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.

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