Sheep IgG HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
HAF016
Detection of SheepIgG Primary Antibody by Western Blot
2 Images
Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (6)

Sheep IgG HRP-conjugated Antibody Summary

Species Reactivity
Sheep
Specificity
Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Sheep IgG
Formulation
Sodium Phosphate and Proclin 300.
Label
HRP (Horseradish Peroxidase)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 
Simple Western
Use at a 1:50 dilution.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of SheepIgG Primary Antibody by Western Blot View Larger

Detection of SheepIgG Primary Antibody by Western Blot Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF7565)using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody(Catalog # HAF016).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

Simple Western Detection of Sheep IgG Primary Antibody by Simple WesternTM. View Larger

Detection of Sheep IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody
(Catalog #AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Sheep IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Association of cardiac myosin binding protein-C with the ryanodine receptor channel: putative retrograde regulation?
    Authors: PJ Stanczyk, M Seidel, J White, C Viero, CH George, S Zissimopou, FA Lai
    J. Cell. Sci., 2018;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: WB
  2. Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease
    Authors: JM Van Kampen, DG Kay
    PLoS ONE, 2017;12(8):e0182896.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  3. Polymerization-Incompetent Uromodulin in the Pregnant Stroke-Prone Spontaneously Hypertensive Rat
    Authors: S Mary, HY Small, J Siwy, W Mullen, A Giri, C Delles
    Hypertension, 2017;69(5):910-918.
    Species: Rat
    Sample Types: Protein
    Applications: WB
  4. UBASH3A Mediates Risk for Type 1 Diabetes through Inhibition of T-Cell Receptor-Induced NF-?B Signaling
    Authors: Y Ge, TK Paisie, JRB Newman, LM McIntyre, P Concannon
    Diabetes, 2017;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: WB
  5. Rnd3-induced cell rounding requires interaction with Plexin-B2
    J Cell Sci, 2016;0(0):.
    Species: Primate - Chlorocebus sabaeus (Green Monkey)
    Sample Types: Cell Lysates
    Applications: WB
  6. Targeting tachykinin receptors in neuroblastoma
    Oncotarget, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  7. Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function
    J Neurosci, 2016;36(35):9201-16.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  8. A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment.
    Authors: Nyegaard M, Rendtorff N, Nielsen M, Corydon T, Demontis D, Starnawska A, Hedemand A, Buniello A, Niola F, Overgaard M, Leal S, Ahmad W, Wikman F, Petersen K, Cruger D, Oostrik J, Kremer H, Tommerup N, Frodin M, Steel K, Tranebjaerg L, Borglum A
    PLoS Genet, 2015;11(7):e1005386.
    Species: Human
    Sample Types: Cell Lysates
  9. A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies.
    Authors: Althoff K, Beckers A, Bell E, Nortmeyer M, Thor T, Sprussel A, Lindner S, De Preter K, Florin A, Heukamp L, Klein-Hitpass L, Astrahantseff K, Kumps C, Speleman F, Eggert A, Westermann F, Schramm A, Schulte J
    Oncogene, 2015;34(26):3357-68.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: WB
  10. Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis.
    Authors: Bose C, Megyesi J, Shah S, Hiatt K, Hall K, Karaduta O, Swaminathan S
    PLoS ONE, 2015;10(8):e0136563.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  11. Antibodies that detect O-linked beta-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins.
    Authors: Tashima Y, Stanley P
    J Biol Chem, 2014;289(16):11132-42.
  12. ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
    Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
    Exp Cell Res, 2014;323(2):263-75.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  13. Bacterial delivery of Staphylococcus aureus alpha-hemolysin causes regression and necrosis in murine tumors.
    Authors: St Jean A, Swofford C, Panteli J, Brentzel Z, Forbes N
    Mol Ther, 2014;22(7):1266-74.
    Species: Bacteria - E. Coli
    Sample Types: Cell Lysates
    Applications: WB
  14. SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/beta-catenin signaling.
    Authors: Thu K, Radulovich N, Becker-Santos D, Pikor L, Pusic A, Lockwood W, Lam W, Tsao M
    Oncogene, 2014;33(3):279-88.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  15. Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions.
    Authors: Nakamura R, Nakamoto C, Obama H
    J. Biol. Chem., 2012;287(5):3282-91.
    Species: Sheep
    Sample Types: Antibody
    Applications: WB
  16. Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription.
    Authors: Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A
    PLoS ONE, 2012;7(4):e34703.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  17. Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
    Authors: Bekku Y, Oohashi T,
    Arch. Histol. Cytol., 2010;73(2):95-102.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Sprouting angiogenesis is regulated by shedding of the C-type lectin family 14, member A (CLEC14A) ectodomain, catalyzed by rhomboid-like 2 protein (RHBDL2).
    Authors: Noy P, Swain R, Khan K, Lodhia P, Bicknell R
    FASEB J, 0;30(6):2311-23.
    Species: Human
    Sample Types: Protein
    Applications: WB
  19. Blocking CLEC14A-MMRN2 binding inhibits sprouting angiogenesis and tumour growth.
    Authors: Noy P, Lodhia P, Khan K, Zhuang X, Ward D, Verissimo A, Bacon A, Bicknell R
    Oncogene, 0;34(47):5821-31.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Reviews for Sheep IgG HRP-conjugated Antibody

Average Rating: 4.8 (Based on 6 Reviews)

5 Star
83.33%
4 Star
16.67%
3 Star
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Sheep IgG HRP-conjugated Antibody
By Anonymous on 07/15/2018
Application: WB Sample Tested: HepG2 human hepatocellular carcinoma cell line Species: Human

Western blotting detection of CD47 using sheep polyclonal antibody (AF4670).


Sheep IgG HRP-conjugated Antibody
By Anonymous on 05/01/2018
Application: ELISA Sample Tested: Serum Species: Sheep

Sheep IgG HRP-conjugated Antibody
By Anonymous on 11/15/2017
Application: WB Sample Tested: Calu-6 human lung carcinoma cell line,H1299 human lung carcinoma cell line Species: Human

Primary Ab = Anti SSBP1 Polyclonal Sheep IgG AF6588 (1:200)
Secondary Ab = 1:1000


Sheep IgG HRP-conjugated Antibody
By Anonymous on 10/23/2017
Application: ELISA Sample Tested: Human recombinant antibody Species: Human

Sheep IgG HRP-conjugated Antibody
By Anonymous on 06/13/2017
Application: WB Sample Tested: HASMC human aortic smooth muscle cells Species: Human

dil: 1:6000 in 5% skim milk powder and TBST.


Sheep IgG Horseradish Peroxidase-conjugated Antibody
By Anonymous on 07/27/2016
Application: WB Sample Tested: Tissue sections Species: Sheep