Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Contactin-6 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Contactin-6
Glu20-Ser998
Accession # Q9JMB8

Specificity

Detects mouse Contactin-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Contactin‑6 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) Contactin-3, rmContactin-4, rmContactin‑5, and rhTAG-1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Contactin‑6 Antibody

Detection of Mouse Contactin-6 antibody by Western Blot.

Detection of Mouse Contactin‑6 by Western Blot.

Western blot shows lysates of mouse brain (cortex) tissue, mouse brain (hypothalamus) tissue, and mouse brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Contactin-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5890) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Contactin-6 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Contactin-6 antibody in Mouse Spinal Cord by Immunohistochemistry (IHC-Fr).

Contactin‑6 in Mouse Spinal Cord.

Contactin-6 was detected in perfusion fixed frozen sections of adult mouse spinal cord using Sheep Anti-Mouse Contactin-6 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF5890) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to gray matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Contactin-6 antibody by Simple WesternTM.

Detection of Mouse Contactin‑6 by Simple WesternTM.

Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin-6 at approximately 168 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Contactin-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5890) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse Contactin‑6 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of adult mouse spinal cord

Simple Western

10 µg/mL
Sample: Mouse brain cortex tissue and mouse brain cerebellum tissue

Western Blot

1 µg/mL
Sample: Mouse brain (cortex) tissue, mouse brain (hypothalamus) tissue, and mouse brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Contactin-6

Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1, 2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3-5). Mouse Contactin-6 shares 90% and 96% amino acid (aa) sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17 aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3, 5-7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6, 8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).

References

  1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  2. Hu, Q.D. et al. (2006) Dev. Neurosci. 28:25.
  3. Lee, S. et al. (2000) Gene 245:253.
  4. Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
  5. Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
  6. Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
  7. Sakurai, K. et al. (2009) Dev. Neurobiol. Aug 11 epub.
  8. Ye, H. et al. (2008) EMBO J. 27:188.

Alternate Names

CNTN6, Contactin6, NB3

Entrez Gene IDs

27255 (Human); 53870 (Mouse); 27256 (Rat)

Gene Symbol

CNTN6

UniProt

Q9JMB8 (Mouse)

Additional Contactin-6 Products

Product Documents for Mouse Contactin‑6 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Contactin‑6 Antibody

For research use only

Related Research Areas

Notch Pathway

Citations for Mouse Contactin‑6 Antibody

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Protocols

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