Mouse Contactin-6 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Contactin-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Contactin‑6 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) Contactin-3, rmContactin-4, rmContactin‑5, and rhTAG-1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Contactin-6
    Glu20-Ser998
    Accession # Q9JMB8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Contactin‑6 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, mouse brain (hypothalamus) tissue, and mouse brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Contactin‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5890) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Contactin‑6 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
Contactin‑6 in Mouse Spinal Cord. Contactin‑6 was detected in perfusion fixed frozen sections of adult mouse spinal cord using Sheep Anti-Mouse Contactin‑6 Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF5890) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to gray matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Contactin‑6 by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑6 at approximately 168 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Contactin‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5890) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Contactin-6
Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1, 2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3-5). Mouse Contactin-6 shares 90% and 96% amino acid (aa) sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17 aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3, 5-7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6, 8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).
  • References:
    1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
    2. Hu, Q.D. et al. (2006) Dev. Neurosci. 28:25.
    3. Lee, S. et al. (2000) Gene 245:253.
    4. Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
    5. Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
    6. Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
    7. Sakurai, K. et al. (2009) Dev. Neurobiol. Aug 11 epub.
    8. Ye, H. et al. (2008) EMBO J. 27:188.
  • Entrez Gene IDs:
    27255 (Human); 53870 (Mouse); 27256 (Rat)
  • Alternate Names:
    CNTN6; contactin 6; Contactin6; Contactin-6; MGC133256; NB3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Loss of NB-3 aggravates cerebral ischemia by impairing neuron survival and neurite growth.
    Authors: Huang X, Sun J, Zhao T
    Stroke, 2011;42(10):2910-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Cell and Tissue Staining Kits
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Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Antigen Retrieval Reagent Sampler (50 mL each)

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Antigen Retrieval Reagent-Acidic

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NorthernLights Guard Mounting Media

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VisUCyte Antigen Retrieval Reagent-Basic

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VisUCyte Antigen Retrieval Reagent-Universal

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Isotype Controls
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Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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