Mouse Contactin-6 Antibody Summary
Accession # Q9JMB8
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Contactin‑6 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, mouse brain (hypothalamus) tissue, and mouse brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Contactin-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5890) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Contactin-6 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Contactin‑6 in Mouse Spinal Cord. Contactin-6 was detected in perfusion fixed frozen sections of adult mouse spinal cord using Sheep Anti-Mouse Contactin-6 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF5890) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to gray matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Contactin‑6 by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin-6 at approximately 168 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Contactin-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5890) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1, 2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3-5). Mouse Contactin-6 shares 90% and 96% amino acid (aa) sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17 aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3, 5-7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6, 8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).
- Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
- Hu, Q.D. et al. (2006) Dev. Neurosci. 28:25.
- Lee, S. et al. (2000) Gene 245:253.
- Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
- Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
- Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
- Sakurai, K. et al. (2009) Dev. Neurobiol. Aug 11 epub.
- Ye, H. et al. (2008) EMBO J. 27:188.
Citations for Mouse Contactin-6 Antibody
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Citations: Showing 1 - 2
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Association of Cell Adhesion Molecules Contactin-6 and Latrophilin-1 Regulates Neuronal Apoptosis
Authors: Amila Zuko
Front Mol Neurosci, 2016;9(0):143.
Sample Types: Tissue Homogenates
Loss of NB-3 aggravates cerebral ischemia by impairing neuron survival and neurite growth.
Authors: Huang X, Sun J, Zhao T
Sample Types: Whole Cells
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