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Simple Plex Human TGF-beta 1 Cartridge

R&D Systems | Catalog # SPCKB-PS-001369

Simple Plex Human TGF-beta 1 assay kit contains cartridge, sample diluent SD21, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL)

Sensitivity

5.29 pg/mL

Assay Range

20.8-12684 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Simple Plex Human TGF-beta 1 Cartridge Summary

Simple Plex assay is for the detection of human Transforming Growth Factor beta 1 (TGF-beta 1) in cell culture supernatant (CCS), serum, and platelet-poor plasma (EDTA/Heparin) in Sample Diluent SD21.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TGF-beta 1 Simple Plex »

Compatible Instruments

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Ella Automated Immunoassay System

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human TGF-beta 1.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Sample Values

Refer to data sheet for sample activation procedures.
TGF-beta 1 Sample Activation Kit DY010 is also available for purchase.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TGF-beta 1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each TGF-beta 1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TGF-beta 1.

Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 25 25
Mean (pg/mL) 111 5909 102 5235
Standard Deviation 6.47 316 11.1 477
CV% 5.8 5.4 10.9 9.1

Recovery for Simple Plex Human TGF-beta 1 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Platelet-poor EDTA Plasma (n=4) 101 90-118
Platelet-poor Heparin Plasma (n=4) 91 83-95

Linearity

Samples containing and/or spiked with high concentrations of human TFG-beta 1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human TGF-beta 1 Assay Linearity

Scientific Data Images for Simple Plex Human TGF-beta 1 Cartridge

Simple Plex Human TGF-beta 1 Assay Standard Curve

Simple Plex Human TGF-beta 1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TGF-beta 1 Assay. Assay range is 20.8-12,684 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 1

TGF-beta 1 (Transforming Growth Factor beta 1) is a pleiotrophic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. TGF-beta 1 associates with LAP to form a latent complex. It can be is activated from latency by plasmin, matrix metalloproteases, thrombospondin 1, and a subset of integrins. TGF-beta 1 signals through complexes of TGF-beta RII with TGF-beta RI/ALK-5 or ALK-1. TGF-beta signaling is modulated by the accessory receptors TGF-beta RIII/Betaglycan and Endoglin/CD105.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for Simple Plex Human TGF-beta 1 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human TGF-beta 1 Cartridge

For research use only

Citations for Simple Plex Human TGF-beta 1 Cartridge

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FAQs for Simple Plex Human TGF-beta 1 Cartridge

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  • Q: Why are RD5P and SD21 diluents included on the TGF-beta 1 control CoA?

    A: RD5P (diluted to 1X) should be used for preparation of high and low controls per the Simple Plex Control Insert. SD21 should be used for standard curve preparation or for spiking samples. Storage stability of TGF-beta 1 in SD21 has not been evaluated, so it is recommended to always use freshly reconstituted TGF-beta 1 for preparations requiring SD21. 

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