Simple Plex Mouse/Rat TGF-beta 1 Cartridge

Name: Simple Plex Mouse/Rat TGF-beta 1 Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-001191
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-001191

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Product Documents

Simple Plex Mouse/Rat TGF-beta 1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD21, and wash buffer.

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Compatible Instruments
Product Specifications
Sample Values
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL)

Sensitivity

5.29 pg/mL

Assay Range

20.8-12684 pg/mL (Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Simple Plex Mouse/Rat TGF-beta 1 Cartridge Summary

Simple Plex assay is for the detection of mouse and rat Transforming Growth Factor beta 1 (TGF-beta 1) in serum and platelet-poor plasma (EDTA/Heparin) in Sample Diluent SD21.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TGF-beta 1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse, Rat

Specificity

Natural and recombinant mouse/rat TGF-beta 1.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Refer to data sheet for sample activation procedures.

TGF-beta 1 Sample Activation Kit DY010 aslo available for purchase.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TGF-beta 1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each TGF-beta 1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for TGF-beta 1.

Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	28	28
Mean (pg/mL)	111	5909	102	5235
Standard Deviation	6.47	316	11.1	477
CV%	5.8	5.4	10.9	9.1

Recovery for Simple Plex Mouse/Rat TGF-beta 1 Cartridge

Recovery was not necessary as mouse/rat TGF-beta 1 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat TFG- beta 1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Mouse TGF-beta 1 Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Mouse/Rat TGF-beta 1 Cartridge

Simple Plex Mouse/Rat TGF-beta 1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat TGF-beta 1 Assay. Assay range is 20.8-12,684 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 1

TGF-beta 1 (Transforming Growth Factor beta 1) is a pleiotrophic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. TGF-beta 1 associates with LAP to form a latent complex. It can be is activated from latency by plasmin, matrix metalloproteases, thrombospondin 1, and a subset of integrins. TGF-beta 1 signals through complexes of TGF-beta RII with TGF-beta RI/ALK-5 or ALK-1. TGF-beta signaling is modulated by the accessory receptors TGF-beta RIII/Betaglycan and Endoglin/CD105.