SIN3A Antibody

Novus Biologicals | Catalog # NB600-1263

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Flow Cytometry, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SIN3A Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) K R R L D D Q E S D V Y A A Q Q R R(19) of mouse mSin3A.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23280436).

Specificity

mSin 3A

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit SIN3A Antibody (NB600-1263) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF, IP and ChIP. Anti-SIN3A Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SIN3A Antibody

Western Blot: SIN3A Antibody [NB600-1263]

Western Blot: SIN3A Antibody [NB600-1263]

Western Blot: SIN3A Antibody [NB600-1263] - Analysis of K562 cell extract.
Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263]

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263]

Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of A431 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: SIN3A Antibody [NB600-1263]

Immunohistochemistry-Paraffin: SIN3A Antibody [NB600-1263]

Immunohistochemistry-Paraffin: SIN3A Antibody [NB600-1263] - Analysis showing staining in the nucleus of human ovary carcinoma (right) compared to a negative control without primary antibody (left).
Chromatin Immunoprecipitation: SIN3A Antibody [NB600-1263] - Analysis of Sin3A performed using cross-linked chromatin from colon carcinoma cells treated with serum. Immunoprecipitation performed using a multiplex microplate Matrix ChIP assay with Sin3A polyclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. A representation of the Egr-1 locus is shown; boxes represent exons (black boxes = translated, white boxes = untranslated), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.
Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263]

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263]

Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263]

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263]

Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).

Applications for SIN3A Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1-3 ul

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

0.5 ug/ml
Application Notes
Use in FLOW reported in scientific literature (PMID 28260693).

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SIN3A

Mammalian Sin 3 (mSin 3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin 3A and mSin 3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin 3A and mSin 3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC 1, HDAC 2, RbAp 46, the methyl CpG binding protein MeCP 2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).

Long Name

SIN3 Homolog A

Alternate Names

DKFZp434K2235, FLJ90319, Histone deacetylase complex subunit Sin3a, KIAA0700, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator (yeast), SIN3 homolog A, transcriptional regulator (yeast), Transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A

Entrez Gene IDs

25942 (Human); 20466 (Mouse)

Gene Symbol

SIN3A

UniProt

Q96ST3 (Human), Q60520 (Mouse)

Additional SIN3A Products

Product Documents for SIN3A Antibody

Certificate of Analysis

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Product Specific Notices for SIN3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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