SLC39A7/ZIP7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76504

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SLC39A7/ZIP7 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human SLC39A7/ZIP7. The immunogen is located within amino acids 20-70 of SLC39A7/ZIP7. Amino Acid Sequence: HDDLQEDFHGHSHRHSH

Specificity

Human SLC39A7/ZIP7 has one isoform (469aa, 50kD). Mouse SLC39A7/ZIP7 has one isoform (476aa, 51kD) and Rat SLC39A7/ZIP7 also has one isoform (468aa, 50kD). NBP1-76504 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SLC39A7/ZIP7 Antibody - BSA Free

Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Rat Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry/ Immunofluorescence: SLC39A7/ZIP7 Antibody - BSA Free [NBP1-76504]

Immunocytochemistry/ Immunofluorescence: SLC39A7/ZIP7 Antibody - BSA Free [NBP1-76504]

Immunocytochemistry/Immunofluorescence: SLC39A7/ZIP7 Antibody [NBP1-76504] - Immunofluorescence of ZIP7 in human brain tissue with ZIP7 antibody at 20 ug/mL.
Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (1 ug/mL), ZIP7 from Company B (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: ZIP7, NBP1-76504 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 AntibodyBSA Free [NBP1-76504]

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
SLC39A7/ZIP7 Antibody - BSA Free

Immunohistochemistry: SLC39A7/ZIP7 Antibody - BSA Free [NBP1-76504] -

Immunohistochemistry: SLC39A7/ZIP7 Antibody - BSA Free [NBP1-76504] - Figure 6 Immunohistochemistry Validation of SLC39A7/ZIP7 in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SLC39A7/ZIP7 antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for SLC39A7/ZIP7 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC39A7/ZIP7

The zinc transporter ZIP7, also known as SLC39A7, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP7 was initially identified while characterizing genes in the major histocompatibility complex on chromosome 17. ZIP7 mRNA is abundantly and widely expressed and the protein localizes to the Golgi apparatus. It functions to transport intracellular zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 expression is expressed by zinc. ZIP7 has been suggested to act a hub for tyrosine kinase activation and may thus be a potential therapeutic target for diseases such as cancer where prevention of tyrosine kinase activation would be advantageous.

Alternate Names

D6S115E, D6S2244EReally interesting new gene 5 protein, H2-KE4, HKE4Solute carrier family 39 member 7, HLA class II region expressed gene KE4, KE4, Ke4 gene, mouse, human homolog of, RING5Histidine-rich membrane protein Ke4, solute carrier family 39 (zinc transporter), member 7, zinc transporter SLC39A7, ZIP7

Gene Symbol

SLC39A7

UniProt

NP_001070984 (Human)

Additional SLC39A7/ZIP7 Products

Product Documents for SLC39A7/ZIP7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SLC39A7/ZIP7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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