SMAC/Diablo Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76772

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMAC/Diablo Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine Smac. The immunogen is located within the last 50 amino acids of Smac.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMAC/Diablo Antibody - BSA Free

Western Blot: SMAC/Diablo AntibodyBSA Free [NBP1-76772]

Western Blot: SMAC/Diablo AntibodyBSA Free [NBP1-76772]

Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NBP1-76772 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - BSA Free [NBP1-76772]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - BSA Free [NBP1-76772]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-76772] - Immunohistochemical staining of mouse spleen tissue using Smac antibody at 2 ug/ml.
Western Blot: SMAC/Diablo AntibodyBSA Free [NBP1-76772]

Western Blot: SMAC/Diablo AntibodyBSA Free [NBP1-76772]

Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 ug /ml.
Western Blot: SMAC/Diablo AntibodyBSA Free [NBP1-76772]

Western Blot: SMAC/Diablo AntibodyBSA Free [NBP1-76772]

Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NB100-55900 (1 ug/mL), SMAC/Diablo NBP1-76772 (1 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
SMAC/Diablo Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody - BSA Free [NBP1-76772] -

Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 4 Immunofluorescence Validation of SMAC/Diablo in Mouse Spleen Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Spleen Cells labeling SMAC/Diablo with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
SMAC/Diablo Antibody - BSA Free

Immunohistochemistry: SMAC/Diablo Antibody - BSA Free [NBP1-76772] -

Immunohistochemistry: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 5 Immunohistochemistry Validation of SMAC/Diablo in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using anti-SMAC/Diablo antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for SMAC/Diablo Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 ug/mL

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAC/Diablo

The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Gene Symbol

DIABLO

UniProt

AF203914 (Mouse)

Additional SMAC/Diablo Products

Product Documents for SMAC/Diablo Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMAC/Diablo Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for SMAC/Diablo Antibody - BSA Free

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail