Smad3 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56479

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Primate

Cited:

Human, Mouse, Canine

Predicted:

Chicken (90%), Xenopus (90%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Smad3 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smad3 Antibody - BSA Free

Western Blot: Smad3 Antibody [NB100-56479]

Western Blot: Smad3 Antibody [NB100-56479]

Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Smad3 Antibody [NB100-56479]

Immunocytochemistry/ Immunofluorescence: Smad3 Antibody [NB100-56479]

Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Smad3 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479]

Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479]

Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/mL.
Western Blot: Smad3 Antibody [NB100-56479]

Western Blot: Smad3 Antibody [NB100-56479]

Western Blot: Smad3 Antibody [NB100-56479] - Analysis using SMAD3 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver and 4) rat liver probed with SMAD3 antibody at 0.5 ug/mL. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Smad3 Antibody - BSA Free

Western Blot: Smad3 Antibody - BSA Free [NB100-56479] -

Effects of Sr and SB-505124 on the transforming growth factor beta (TGF beta )/SMAD family member (SMAD) signaling pathway and downstream gene transcription in bovine chondrocytes. Chondrocytes were treated with different doses of Sr (0, 0.1, 1, 10 μg/ml) with or without activin receptor-like kinase 5 (ALK5) kinase inhibitor (10 μM SB-505124). (A) Western blot analysis and relative protein expression levels of SMAD3, pSMAD3, SMAD1/5/9, pSMAD1/5/9, TGF beta, bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2). (B) mRNA expression levels of TGF beta 1, serpin family E member 1 (SERPINE1), ALK5, ALK1, and inhibitor of DNA binding 1 (ID1). All experiments were repeated at least thrice. Data are means +/- SEM (n = 3 in each group) *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35712700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Smad3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Simple Western

1:50

Western Blot

0.5 - 2 ug/mL
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Bladder, separated by Size, antibody dilution of 1:50

Reviewed Applications

Read 5 reviews rated 3.2 using NB100-56479 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad3

SMAD3, a member of the SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain. It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. SMAD3 is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia.

Long Name

Mothers Against DPP Homolog 3

Alternate Names

DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3

Entrez Gene IDs

17127 (Mouse); 25631 (Rat)

Gene Symbol

SMAD3

UniProt

NP_005893 (Human)

Additional Smad3 Products

Product Documents for Smad3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Smad3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Smad3 Antibody - BSA Free

Customer Reviews for Smad3 Antibody - BSA Free (5)

3.2 out of 5
5 Customer Ratings
5 Stars
40%
4 Stars
0%
3 Stars
20%
2 Stars
20%
1 Stars
20%

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Smad3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 08/02/2021
    Analysis of smad3 in lung tissue.
    Smad3 Antibody - BSA Free NB100-56479
  • Smad3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: eye tissue
    Species: mice
    Verified Customer | Posted 05/26/2021
    poor quality
    used at 1:100 overnight.
    Smad3 Antibody - BSA Free NB100-56479
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Smad3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: eye tissue
    Species: Bovine
    Verified Customer | Posted 05/26/2021
    works just fine
    Used at 1:1000- for overnight.
    Smad3 Antibody - BSA Free NB100-56479
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Smad3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: ocular tissue
    Species: Human
    Verified Customer | Posted 05/24/2021
    Gives multiple bands
    used at 1:1000 and incubated at 4degrees overnight
    Smad3 Antibody - BSA Free NB100-56479
  • Smad3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human fibroblast and HeLa whole cell lysate
    Species: Mouse
    Verified Customer | Posted 06/17/2019
    untreated whole cell lysate band observed around 55 Kda
    1:1000 dilution 1 hour blocking in milk 5%
    Smad3 Antibody - BSA Free NB100-56479

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Protocols

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FAQs for Smad3 Antibody - BSA Free

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  • Q: I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?

    A: We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

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