SMN Antibody (2B1) - BSA Free

Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936]

SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0010.jpg

Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936]

Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with SMN Antibody [2B1] conjugated to Alexa Fluor 488 (NB100-1936AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: SMN Antibody (2B1) [NB100-1936]

Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Neuro2a cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936]

Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against DyLight 488 (Green). Actin nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.

Immunohistochemistry: SMN Antibody (2B1) [NB100-1936]

Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN on mouse brain using NB100-1936.

Flow Cytometry: SMN Antibody (2B1) [NB100-1936]

Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Ntera2 cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

SMN (2B1) in U-2 OS Human Cell Line -

SMN (2B1) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-SMN (2B1) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB100-1936AF647) (light blue) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

Detection of SMN (2B1) in A431 Human Cell Line by Flow Cytometry.

An intracellular stain was performed on A431 human skin carcinoma cell line using Mouse anti- SMN (2B1) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB100-1936AF647, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.

Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] -

Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - CWC22 is colocalized with nuclear speckles & upregulated in diabetic DRG sensory neurons. (A) Subcellular distribution of TDP-43 & CWC22 in control DRG sensory neurons. TDP-43 was stained diffusely in the nucleus, excluding SMN foci in sensory neurons. CWC22 consistently colocalized with a marker protein SC35 of nuclear speckles in sensory neurons. No obvious differences in the subcellular localization of CWC22 were identified in diabetic neurons (not shown) compared with controls. Scale bar: 10 μm. (B) qRT-PCR analysis of Cwc22 mRNA expression in diabetic & control mice. Cwc22 expression was upregulated ∼2.5-fold in diabetic DRGs. *P<0.05, unpaired two-tailed Student's t-test. Data represented as mean±s.e.m. See Cheng et al. (2015) for microarray data indicating rises in Cwc22 expression as reported separately. Image collected & cropped by CiteAb from the following publication (https://journals.biologists.com/dmm/article/10/3/215/2257/Diabetic-poly…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

SMN (2B1) in A431 Human Cell Line.

SMN (2B1) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti-SMN (2B1) Protein G Purified Monoclonal Antibody conjugated to Biotin (Catalog # NB100-1936B) at 5 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

SMN (2B1) in U-2 OS Human Cell Line.

SMN (2B1) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-SMN (2B1) Protein G Purified Monoclonal Antibody conjugated to DyLight 488 (Catalog # NB100-1936G) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

SMN (2B1) in U-2 OS Human Cell Line.

SMN (2B1) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-SMN (2B1) Protein G Purified Monoclonal Antibody conjugated to FITC (Catalog # NB100-1936F) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: SMN Antibody (2B1) - BSA Free [NB100-1936] -

Spinal irisin gene delivery attenuated burn injury-induced atrophy markers and reduced irisin and SMN expression in ipsilateral gastrocnemius muscle. (A) Immunofluorescence staining of atrogin-1 and MuRF in ipsilateral gastrocnemius muscle in the fourth week after burn injury. DAPI counterstain was used to locate the nucleus. (B,C) Representative bar graphs illustrating averaged optical intensity of atrogin-1 and MuRF. Error bars represent standard deviations (SDs). ** p < 0.01, *** p < 0.001, unpaired t-test. (D) Immunoblot of ipsilateral gastrocnemius muscle tissue in the fourth week after burn injury. (E–H) Representative bar graphs illustrating normalized expression ratio of irisin, atrogin-1, MuRF, and SMN, with GAPDH as an internal control. Error bars represent SDs. * p < 0.05, ** p < 0.01, unpaired t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36555538), licensed under a CC-BY license. Not internally tested by Novus Biologicals.