SOD2/Mn-SOD Antibody - BSA Free

Novus Biologicals | Catalog # NB100-1992

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila, Goat, Guinea Pig, Hamster, Monkey, Rabbit, Sheep, Squirrel, Xenopus

Cited:

Human, Mouse, Rat, Avian - Chicken, Goat, Hamster, Insect - Drosophila, Ovine, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOD2/Mn-SOD Primary Antibodies »

Product Specifications

Immunogen

Recombinant Rat Mn SOD Protein

Reactivity Notes

Goat reactivity reported in scientific literature (PMID: 28797922). Reacts with Ciona intestinalis (Sea squirt).

Localization

Mitochondrial matrix

Specificity

Detects 25 kDa protein, corresponding to the molecular mass of Mn superoxide dismutase (SOD) on SDS-PAGE immunoblots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOD2/Mn-SOD Antibody - BSA Free

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992]

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992]

SOD2-Mn-SOD-Antibody-Immunohistochemistry-NB100-1992-img0013.jpg
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992]

Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992]

Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.
Western Blot: SOD2/Mn-SOD Antibody [NB100-1992]

Western Blot: SOD2/Mn-SOD Antibody [NB100-1992]

Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Western blot analysis of Rat Tissue lysates showing detection of SOD2/Mn-SOD protein using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992]

Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992]

Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody at 1:120 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite.
Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992]

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992]

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - Immunohistochemistry analysis using Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2/Mn-SOD Polyclonal Antibody (NB100-1992) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix.
SOD2/Mn-SOD Antibody

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] -

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SOD2/Mn-SOD Antibody

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] -

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SOD2/Mn-SOD Antibody

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] -

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SOD2/Mn-SOD Antibody

Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] -

Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - CAV-1 preserves mitochondrial respiratory function through regulation of OXPHOS expression.(A) OXPHOS complex subunits were detected through western blotting with appropriate antibodies. CAV-1 treatment significantly restored hypercholesterolemia-associated increased ETC complex I–V proteins levels. (B) Percentage of the OXPHOS complex (I–V) band intensities is presented in the graph. (C) Representative immunoblot displaying levels of CAV-1, CyPA, mitochondrial biogenesis markers, & antioxidant enzymes in the NC, HC, & HCAV-1 groups. (D) Columns represent average values over three independent experiments. The density for the NC group was set at 1; * & † are significantly different for the NC & HC groups, respectively, at P < 0.05. beta -actin was used as a loading control. Values are means ± SD. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0154210), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SOD2/Mn-SOD Antibody

Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] -

Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SOD2/Mn-SOD Antibody

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] -

Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SOD2/Mn-SOD Antibody

Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] -

Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - SOD2 mRNA & protein expression. RT-PCR: (A) SOD2 mRNA expression is higher in adults, & highest in the adult parenchyma. (B) No exposure effects on SOD2 mRNA were observed in neonates. (C) Adult SOD2 mRNA was decreased in PFP48. Data are presented as mean+SEM (n=5-7 rats/group, in each compartment), * significantly different compared to neonates in the same compartment, † significantly different compared to airways in the same age, ‡ significantly different compared to FA in the same compartment. Western blotting: (D) Scan of representative SOD2 & actin blots. (E) Neonatal whole lung SOD2 protein expression was unchanged with exposure, & (F) adult whole lung SOD2 protein trended upwards at PFP2, but was statistically insignificant. (G-J) Immunohistochemical localization of SOD2 in lung (n=6 rats/group). SOD2 protein was more abundant in adults compared to neonates, but no exposure specific differences were observed. Scale bar is 50 μm. Image collected & cropped by CiteAb from the following publication (https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/1…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SOD2/Mn-SOD Antibody - BSA Free

Simple Western: Rabbit Polyclonal SOD2/Mn-SOD Antibody [NB100-1992]

SOD2 increase after oxidative stress in mouse lung tissue. 1:20 in antibody diluent 2, 60min of incubation. Image from a verified customer review.

Applications for SOD2/Mn-SOD Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:120

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Simple Western

Validated for Simple Western from a verified customer review.

Western Blot

1:5000

Reviewed Applications

Read 2 reviews rated 5 using NB100-1992 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Entrez Gene IDs

6648 (Human); 20656 (Mouse); 24787 (Rat)

Gene Symbol

SOD2

UniProt

P07895 (Rat)

Additional SOD2/Mn-SOD Products

Product Documents for SOD2/Mn-SOD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOD2/Mn-SOD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for SOD2/Mn-SOD Antibody - BSA Free

Customer Reviews for SOD2/Mn-SOD Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
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Customer Images

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  • SOD2 in mouse lung homogenate
    Name: Anonymous
    Application: Simple Western
    Sample Tested: lung homogenate
    Species: Mouse
    Verified Customer | Posted 09/08/2025
    SOD2 protein expression in experimental mouse lung homogenates was determined with JESS simple western
    protein expression of sod2 in the mouse lung homogenate treated with smoke
    SOD2/Mn-SOD Antibody - BSA Free NB100-1992
  • SOD2 in mouse
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 06/03/2025
    SOD2 increase after oxidative stress
    1:20 in antibody diluent 2, 60min of incubation
    SOD2/Mn-SOD Antibody - BSA Free NB100-1992
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SOD2/Mn-SOD Antibody - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I want to measure SOD and Catalase on different erythroid subpopulation by using flow cytometry.I want to know whether these SOD and Catalase molecules are directly conjugated with fluorescence molecules or I have to do indirect staining.I have to use Ter 119 and CD71 molecule along with catalase or SO antibody.Can you please provide me detail protocol for using these antibodis or send me some references, if anyone used these antibodies by utilizing multicolour flow cytometry.

    A: For our testing of NB100-1992, we used conjugated secondary antibodies. We have not yet tried to directly conjugate this primary, although this is certainly possible and would be expected to give the same results. We do not routinely test our antibodies in FACS, and in most cases, this application is validated by a trusted collaborator. For NB100-1992, viability for FACS was reported by a customer, and we do not have a detailed protocol for this application and product.

  • Q: What is your recommended working dilution for IHC?

    A: In regards to the IHC dilution, we would recommend starting at about 1:10-1:2000. Obviously this will depend on your samples and how highly expressed the protein is in them. You may need to optimize, but this is a good starting point.

  • Q: I want to measure SOD and Catalase on different erythroid subpopulation by using flow cytometry.I want to know whether these SOD and Catalase molecules are directly conjugated with fluorescence molecules or I have to do indirect staining.I have to use Ter 119 and CD71 molecule along with catalase or SO antibody.Can you please provide me detail protocol for using these antibodis or send me some references, if anyone used these antibodies by utilizing multicolour flow cytometry.

    A: For our testing of NB100-1992, we used conjugated secondary antibodies. We have not yet tried to directly conjugate this primary, although this is certainly possible and would be expected to give the same results. We do not routinely test our antibodies in FACS, and in most cases, this application is validated by a trusted collaborator. For NB100-1992, viability for FACS was reported by a customer, and we do not have a detailed protocol for this application and product.

  • Q: What is your recommended working dilution for IHC?

    A: In regards to the IHC dilution, we would recommend starting at about 1:10-1:2000. Obviously this will depend on your samples and how highly expressed the protein is in them. You may need to optimize, but this is a good starting point.

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