SOX10 Antibody (8E1) - BSA Free

Novus Biologicals | Catalog # NBP3-26306

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8E1 expressed in HEK293

Format

BSA Free
View all SOX10 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human SOX10 [UniProt P56693]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX10 Antibody (8E1) - BSA Free

SOX10 Antibody (8E1)

Immunohistochemistry: SOX10 Antibody (8E1) [NBP3-26306] -

Immunohistochemistry: SOX10 Antibody (8E1) [NBP3-26306] - Image of SOX10 Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
SOX10 Antibody (8E1)

Immunohistochemistry: SOX10 Antibody (8E1) [NBP3-26306] -

Immunohistochemistry: SOX10 Antibody (8E1) [NBP3-26306] - Image of SOX10 Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
SOX10 Antibody (8E1)

Western Blot: SOX10 Antibody (8E1) [NBP3-26306] -

Western Blot: SOX10 Antibody (8E1) [NBP3-26306] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate.
All lanes: SOX10 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 50, 32 kDa
Observed band size: 60 kDa
SOX10 Antibody (8E1)

Immunocytochemistry/Immunofluorescence: SOX10 Antibody (8E1) [NBP3-26306] -

Immunocytochemistry/Immunofluorescence: SOX10 Antibody (8E1) [NBP3-26306] - Staining of Hela Cells with SOX10 Antibody (8E1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for SOX10 Antibody (8E1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SOX10

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Long Name

SRY-box transcription factor 10

Alternate Names

DOM, PCWH, SOX-10, WS2E, WS4, WS4C

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for SOX10 Antibody (8E1) - BSA Free

Certificate of Analysis

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Product Specific Notices for SOX10 Antibody (8E1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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