SOX9 Antibody (SN74-09)

Flow Cytometry: SOX9 Antibody (SN74-09) [NBP2-67690]

Flow Cytometry: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of Hela cells with SOX9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690]

Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690]

Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody at 1/500 dilution.Lane 1: SW480 cell lysateLane 2: A431 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 56 kDaObserved band size: 70 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunocytochemistry/Immunofluorescence: SOX9 Antibody (SN74-09) [NBP2-67690] - Staining SOX9 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-SOX9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human tonsil tissue using anti-SOX9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded mouse colon tissue using anti-SOX9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded mouse spleen tissue using anti-SOX9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human thyroid tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690]

Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.