Src Antibody (5A18) - BSA Free

Name: Src Antibody (5A18) - BSA Free
Brand: Novus Biologicals
SKU: NBP1-19188
Price: 329 USD
Availability: InStock
Rating: 5 (2 reviews)

Novus Biologicals | Catalog # NBP1-19188

(2)

Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse Clone # 5A18

Format

BSA Free

View all Src Primary Antibodies »

Product Specifications

Immunogen

Recombinant human full length c-Src

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

Src (5A18)

Clonality

Monoclonal

Host

Mouse

Description

Novus Biologicals Mouse Src Antibody (5A18) - BSA Free (NBP1-19188) is a monoclonal antibody validated for use in IHC, WB and Simple Western. Anti-Src Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Src Antibody (5A18) - BSA Free

Western Blot: Src Antibody (5A18) [NBP1-19188]

Western Blot: Src Antibody (5A18) [NBP1-19188] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Src at Y419 and total Src were detected by western blot. From a verified customer review.

Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]

Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human prostate tissue stained with Src, mAb (5A18) at 20 ug/ml.

Western Blot: Src Antibody (5A18) [NBP1-19188]

Western Blot: Src Antibody (5A18) [NBP1-19188] - Western blot analysis of Src using 20 ug of whole cell lysate (Lane 2=HeLa, Lane 3=3T3, Lane 4=PC12) probed with with Src, mAb (5A18) at 1 ug/ml.

Immunohistochemistry: Src Antibody (5A18) [NBP1-19188]

Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human adrenal tissue stained with Src, mAb (5A18) at 20 ug/ml.

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Applications for Src Antibody (5A18) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1 ug/ml

Application Notes

WB validation from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: tested in A431 -/+ EGF; antibody dilution of 1:50; separated by size

Reviewed Applications

Read 2 reviews rated 5 using NBP1-19188 in the following applications:

Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Src

Src is a member of the tyrosine kinase family that was originally identified as the oncogene of Rous sarcoma virus. The catalytic domain contains a tyrosine autophosphorylation site that increases kinase activity when phosphorylated. The Src family of tyrosine kinase is involved in a variety of receptor-induced signal transduction pathways such as immune recognition rec

Long Name

v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog

Alternate Names

ASV, c-Src, RSVgp4

Entrez Gene IDs

6714 (Human); 20779 (Mouse); 83805 (Rat)

Gene Symbol

SRC

UniProt

P12931

Additional Src Products

Product Documents for Src Antibody (5A18) - BSA Free

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Src Antibody (5A18) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules on Endothelial Cells

Apoptosis Intracellular Kinases

C-type Lectin Receptors

Cancer Biomarkers

GDNF Family

Intracellular Kinases

Citations for Src Antibody (5A18) - BSA Free

Customer Reviews for Src Antibody (5A18) - BSA Free (2)

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2 Customer Ratings

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Customer Images

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Src Antibody (5A18)

Name: Haiquan Lu

Application: Western Blot

Sample Tested: Breast cancer cells

Species: Human

Verified Customer | Posted 10/11/2018

Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Src at Y419 and total Src were detected by western blot.
Name: Hongxia Hu

Application: Western Blot

Sample Tested: Hela whole cell lysate

Species: Human

Verified Customer | Posted 09/12/2012