STAT1 Antibody (OTI15H3)

Name: STAT1 Antibody (OTI15H3)
Brand: Novus Biologicals
SKU: NBP1-48013
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-48013

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI15H3

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Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human STAT1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse STAT1 Antibody (OTI15H3) (NBP1-48013) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT1 Antibody (OTI15H3)

Western Blot: STAT1 Antibody (OTI15H3) [NBP1-48013]

Western Blot: STAT1 Antibody (15H3) [NBP1-48013] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.

Immunocytochemistry/Immunofluorescence: STAT1 Antibody (15H3) [NBP1-48013] Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.

Immunohistochemistry-Paraffin: STAT1 Antibody (OTI15H3) [NBP1-48013]

Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT1 Antibody (OTI15H3) [NBP1-48013]

Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-STAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT1 Antibody (OTI15H3) [NBP1-48013]

Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-STAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT1 Antibody (OTI15H3) [NBP1-48013]

Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-STAT1 mouse monoclonal antibody.

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Applications for STAT1 Antibody (OTI15H3)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT1

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Entrez Gene IDs

6772 (Human)

Gene Symbol

STAT1

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Product Documents for STAT1 Antibody (OTI15H3)

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Product Specific Notices for STAT1 Antibody (OTI15H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.