Q: We are looking for an NBS mouse monoclonal antibody that would work for IF. NBS1 Antibody (NB100-221) might be ideal, except we need to use it in mouse cells, and the data sheet says it is reactive with human cells. Could you tell us if it has been tested in mouse cells? If it has not, we would be willing to test it, and send you verifying images. However, if we test it and it does not work in mouse cells, would we be able to receive another antibody in exchange? We would also like to purchase a STAT1 mouse monoclonal antibody that would work for IF. STAT1 Antibody (NBP1-48011). The species reactivity of the antibody indicates that it is reactive with human, canine, and primate cells. Has it been tested in mouse cells?

A: I have a couple of thoughts after reading through your needs for this antibody. This antibody is validated for mouse reactivity. I assume you realize that if you want to use a mouse mAb on mouse tissue, you will need to do a mouse on mouse block to prevent non-specific staining with your secondary. If you would like to test an antibody in an unvalidated species or application, please consider our Innovators Reward Program. STAT1, NBP1-48011, has not been validated for mouse reactivity so this would qualify for the Innovators Reward Program.

STAT1 Antibody (OTI4H9)

Novus Biologicals | Catalog # NBP1-48011

(1)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI4H9

View all STAT1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human STAT1 (NP_009330) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

82.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT1 Antibody (OTI4H9)

Western Blot: STAT1 Antibody (OTI4H9) [NBP1-48011]

Western Blot: STAT1 Antibody (OTI4H9) [NBP1-48011] - Analysis of extracts (10ug) from 7 Human tissue by using anti-STAT1 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.

Immunocytochemistry/ Immunofluorescence: STAT1 Antibody (OTI4H9) [NBP1-48011]

Immunocytochemistry/Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.

Immunohistochemistry-Paraffin: STAT1 Antibody (OTI4H9) [NBP1-48011]

Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human Kidney tissue using anti-STAT1 mouse monoclonal antibody.

Western Blot: STAT1 Antibody (OTI4H9) [NBP1-48011]

Western Blot: STAT1 Antibody (4H9) [NBP1-48011] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.

Western Blot: STAT1 Antibody (4H9) [NBP1-48011] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-STAT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/ Immunofluorescence: STAT1 Antibody (OTI4H9) [NBP1-48011]

Immunocytochemistry/Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Immunofluorescent staining of HT29 cells using anti-STAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT1 Antibody (OTI4H9) [NBP1-48011]

Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT1 Antibody (OTI4H9) [NBP1-48011]

Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human breast tissue using anti-STAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT1 Antibody (OTI4H9) [NBP1-48011]

Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human endometrium tissue using anti-STAT1 mouse monoclonal antibody.

View 6 more images

Applications for STAT1 Antibody (OTI4H9)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000

Reviewed Applications

Read 1 review rated 4 using NBP1-48011 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT1

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors,STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Entrez Gene IDs

6772 (Human)

Gene Symbol

STAT1

Additional STAT1 Products

Product Documents for STAT1 Antibody (OTI4H9)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for STAT1 Antibody (OTI4H9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Transcription Factors and Regulators

Interferons

Macrophage Activation Markers

Myeloid-derived Suppressor Cells (MDSC)

PDGF Family

Transcription Factors in the Akt Pathway

Customer Reviews for STAT1 Antibody (OTI4H9) (1)

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Name: rajesha rupaimoole

Application: Western Blot

Sample Tested: Human ovarian cancer cell line A2780

Species: Human

Verified Customer | Posted 10/06/2014

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for STAT1 Antibody (OTI4H9)

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Q: We are looking for an NBS mouse monoclonal antibody that would work for IF. NBS1 Antibody (NB100-221) might be ideal, except we need to use it in mouse cells, and the data sheet says it is reactive with human cells. Could you tell us if it has been tested in mouse cells? If it has not, we would be willing to test it, and send you verifying images. However, if we test it and it does not work in mouse cells, would we be able to receive another antibody in exchange? We would also like to purchase a STAT1 mouse monoclonal antibody that would work for IF. STAT1 Antibody (NBP1-48011). The species reactivity of the antibody indicates that it is reactive with human, canine, and primate cells. Has it been tested in mouse cells?

A: I have a couple of thoughts after reading through your needs for this antibody. This antibody is validated for mouse reactivity. I assume you realize that if you want to use a mouse mAb on mouse tissue, you will need to do a mouse on mouse block to prevent non-specific staining with your secondary. If you would like to test an antibody in an unvalidated species or application, please consider our Innovators Reward Program. STAT1, NBP1-48011, has not been validated for mouse reactivity so this would qualify for the Innovators Reward Program.