STAT1 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-56314
![Western Blot: STAT1 Antibody [NB100-56314]](https://resources.rndsystems.com/images/products/STAT1-Antibody-Western-Blot-NB100-56314-img0002.jpg "Western Blot: STAT1 Antibody [NB100-56314]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Porcine, Canine, Primate
Cited:
Human
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide sequence corresponding to amino acids between 700 and 750 of human Stat1 was used as immunogen. This sequence is 100% conserved between Stat1 alpha and Stat1 beta.
Specificity
This antibody is able to detect both the alpha form (p91, ~91 kD) and the beta form (p84, ~84 kD) of STAT1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit STAT1 Antibody - BSA Free (NB100-56314) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-STAT1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for STAT1 Antibody - BSA Free
Western Blot: STAT1 Antibody [NB100-56314]
Western Blot: STAT1 Antibody [NB100-56314] - Western blot analysis of STAT1 in HeLa cell lysate using NB100-56314.![Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314]](https://resources.rndsystems.com/images/products/STAT1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56314-img0004.jpg "Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314]")
Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314]
Immunocytochemistry/Immunofluorescence: STAT1 Antibody [NB100-56314] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-STAT1 Antibody NB100-56314 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.![Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314]](https://resources.rndsystems.com/images/products/STAT1-Antibody-Immunohistochemistry-Paraffin-NB100-56314-img0003.jpg "Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314]")
Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314]
Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of STAT1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Strong nuclear staining in germinal center and surrounding cells along with cytoplasmic reactivity was observed.Applications for STAT1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-5 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: STAT1
Long Name
Signal Transducer and Activator of Transcription 1
Alternate Names
CANDF7, ISGF-3, STAT91
Entrez Gene IDs
20846 (Mouse)
Gene Symbol
STAT1
UniProt
Additional STAT1 Products
Product Documents for STAT1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for STAT1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for STAT1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
