STAT1 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-56314
![Western Blot: STAT1 Antibody [NB100-56314]](https://resources.rndsystems.com/images/products/STAT1-Antibody-Western-Blot-NB100-56314-img0002.jpg "Western Blot: STAT1 Antibody [NB100-56314]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Loading...
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Porcine, Canine, Primate
Cited:
Human
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
A synthetic peptide sequence corresponding to amino acids between 700 and 750 of human Stat1 was used as immunogen. This sequence is 100% conserved between Stat1 alpha and Stat1 beta.
Specificity
This antibody is able to detect both the alpha form (p91, ~91 kD) and the beta form (p84, ~84 kD) of STAT1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for STAT1 Antibody - BSA Free
Western Blot: STAT1 Antibody [NB100-56314]
Western Blot: STAT1 Antibody [NB100-56314] - Western blot analysis of STAT1 in HeLa cell lysate using NB100-56314.![Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314]](https://resources.rndsystems.com/images/products/STAT1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56314-img0004.jpg "Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314]")
Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314]
Immunocytochemistry/Immunofluorescence: STAT1 Antibody [NB100-56314] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-STAT1 Antibody NB100-56314 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.![Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314]](https://resources.rndsystems.com/images/products/STAT1-Antibody-Immunohistochemistry-Paraffin-NB100-56314-img0003.jpg "Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314]")
Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314]
Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of STAT1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Strong nuclear staining in germinal center and surrounding cells along with cytoplasmic reactivity was observed.Applications for STAT1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-5 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: STAT1
Long Name
Signal Transducer and Activator of Transcription 1
Alternate Names
CANDF7, ISGF-3, STAT91
Entrez Gene IDs
20846 (Mouse)
Gene Symbol
STAT1
UniProt
Additional STAT1 Products
Product Documents for STAT1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for STAT1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for STAT1 Antibody - BSA Free
Powered by Bioz
Customer Reviews for STAT1 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review STAT1 Antibody - BSA Free and earn rewards!
Have you used STAT1 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
