STAT6 Antibody (BLR147J)

Name: STAT6 Antibody (BLR147J)
Brand: Novus Biologicals
SKU: NBP3-14726
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14726

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Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR147J

View all STAT6 Primary Antibodies »

Product Specifications

Immunogen

between 600 and 650

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for STAT6 Antibody (BLR147J)

Immunoprecipitation: STAT6 Antibody (BLR147J) [NBP3-14726]

Immunoprecipitation: STAT6 Antibody [NBP3-14726] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from UACC-62 cells.Antibodies: Rabbit anti-STAT6 recombinant monoclonalantibody used for IP at 20ul/mg lysate. STAT6 was also immunoprecipitated by otherantibodies against different epitopes of STAT6. For blotting immunoprecipitatedSTAT6, was used at 1:1000. Chemiluminescencewith an exposure time of 30 seconds.

Western Blot : STAT6 Antibody [NBP3-14726] -

Western Blot : STAT6 Antibody [NBP3-14726] -Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27,CTLL-2, and BW5147.3 cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-STAT6 recombinantmonoclonal antibody [BLR147J] used at1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.Chemiluminescence with an exposure timeof 75 seconds. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody.

Western Blot: STAT6 Antibody [NBP3-14726] -

Western Blot: STAT6 Antibody [NBP3-14726] - Whole cell lysate (50 µg) from Jurkat, UACC-62, HeLa, NCIH460, and HEK293T cells prepared using NETN lysis buffer.Antibody: Rabbit anti-STAT6 recombinant monoclonalantibody [BLR147J] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.

Immunohistochemistry-Paraffin:STAT6 Antibody [NBP3-14726] -

Immunohistochemistry-Paraffin:STAT6 Antibody [NBP3-14726] -Rabbit anti-STAT6recombinant monoclonal antibody.Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.

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Applications for STAT6 Antibody (BLR147J)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

20 µl/1 mg lysate

Western Blot

1:1,000

Application Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Janus family tyrosine kinases (Jak)/ STAT signal transduction pathway and mediates cytokine signaling by IL 4 and IL-13 (1). STAT6 (predicted molecular weight 94kDa) has been found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL-4. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is tyrosine phosphorylated (Tyr641) in response to cellular interaction with IL-4. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 and Th9 immune response (2). Loss of STAT6 impairs IL-4 mediated functions including Th2 helper T cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. Diseases associated with STAT6 include malignant hemangiopericytoma and nut allergies.

References

1. Waqas, S. F. H., Ampem, G., & Roszer, T. (2019). Analysis of IL-4/STAT6 Signaling in Macrophages. Methods Mol Biol, 1966, 211-224. doi:10.1007/978-1-4939-9195-2_17

2. Goenka, S., & Kaplan, M. H. (2011). Transcriptional regulation by STAT6. Immunol Res, 50(1), 87-96. doi:10.1007/s12026-011-8205-2

Long Name

Signal Transducer and Activator of Transcription 6

Alternate Names

D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT

Gene Symbol

STAT6

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Product Specific Notices for STAT6 Antibody (BLR147J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.