STAT6 Antibody

Name: STAT6 Antibody
Brand: Novus Biologicals
SKU: NBP2-20506
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-20506

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all STAT6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human STAT6. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat, Bovine, Porcine, and Rhesus Macaque (>80% identity).

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit STAT6 Antibody (NBP2-20506) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT6 Antibody

Immunoprecipitation: STAT6 Antibody [NBP2-20506]

Immunoprecipitation: STAT6 Antibody [NBP2-20506] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of STAT6 protein by 2 ug of STAT6 antibody 10% SDS-PAGE The immunoprecipitated STAT6 protein was detected by STAT6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.

Western Blot: STAT6 Antibody [NBP2-20506] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with STAT6 antibody (NBP2-20506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunocytochemistry/ Immunofluorescence: STAT6 Antibody [NBP2-20506] -

STAT6 antibody detects STAT6 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STAT6 stained by STAT6 antibody (NBP2-20506) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for STAT6 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:500-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Janus family tyrosine kinases (Jak)/ STAT signal transduction pathway and mediates cytokine signaling by IL 4 and IL-13 (1). STAT6 (predicted molecular weight 94kDa) has been found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL-4. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is tyrosine phosphorylated (Tyr641) in response to cellular interaction with IL-4. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 and Th9 immune response (2). Loss of STAT6 impairs IL-4 mediated functions including Th2 helper T cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. Diseases associated with STAT6 include malignant hemangiopericytoma and nut allergies.

References

1. Waqas, S. F. H., Ampem, G., & Roszer, T. (2019). Analysis of IL-4/STAT6 Signaling in Macrophages. Methods Mol Biol, 1966, 211-224. doi:10.1007/978-1-4939-9195-2_17

2. Goenka, S., & Kaplan, M. H. (2011). Transcriptional regulation by STAT6. Immunol Res, 50(1), 87-96. doi:10.1007/s12026-011-8205-2

Long Name

Signal Transducer and Activator of Transcription 6

Alternate Names

D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT

Gene Symbol

STAT6

UniProt

P42226

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Product Specific Notices for STAT6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.