StemXVivo Mesenchymal Stem Cell Expansion Media

Catalog # Availability Size / Price Qty
CCM004
Product Details
Procedure
Citations (7)
FAQs
Supplemental Products
Reviews (4)

StemXVivo Mesenchymal Stem Cell Expansion Media Summary

Kit Summary

A complete, fetal bovine serum-containing media formulated and optimized for the maintenance and expansion of purified human, mouse, and rat MSCs.

Key Benefits

  • Formulated to reduce variation
  • All components, including fetal bovine serum, were selected and optimized for MSCs
  • Ready to use
 

 

Why Culture MSCs in a Defined Medium?

Mesenchymal stem/stromal cells (MSCs) are a rare population of multipotent cells that can be derived from a number of tissues such as bone marrow, adipose, and placenta.

Given their rarity and sensitivity to ex vivo conditions, MSCs are most often expanded in vitro under specific culture conditions.

StemXVivo® MSC Expansion Media:

  • Supports reproducible MSC growth in vitro.
  • Can be supplemented with user-defined cytokines and growth factors, although these are not needed for culture
  • Has been developed and optimized for use with human, mouse, and rat MSCs.
 

 

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’

Mesenchymal stem cells are multipotent, self-renewing cells that have the ability to differentiate into adipocytes, chondrocytes, and osteoblasts when cultured in vitro. Read More about MSC Nomenclature

StemXVivo® MSC Expansion Media Components

Supplied in a 250 mL volume, this medium contains high quality factors to support MSC expansion in vitro.

  • Supplemented with sodium bicarbonate
  • Does not contain antibiotics

 

Data Examples
Phenotypic Analysis of Human MSCs Expanded in StemXVivo MSC Expansion Media
View Larger Image

Phenotypic Analysis of Human MSCs Expanded in StemXVivo® MSC Expansion Media. Human MSCs were expanded using StemXVivo® Mesenchymal Stem Cell (MSC) Expansion Media (Catalog # CCM004). Filled histograms indicate cells stained with markers of undifferentiated MSCs including anti-CD105 (Catalog # FAB10971P) or CD45 (Catalog # MAB1430). The open histograms show isotype-matched control staining. MSCs appropriately lack expression of CD45.

Phenotypic Analysis of Mouse MSCs Expanded in StemXVivo MSC Expansion Media
View Larger Image

Phenotypic Analysis of Mouse MSCs Expanded in StemXVivo® MSC Expansion Media. Mouse MSCs were expanded using StemXVivo® Mesenchymal Stem Cell (MSC) Expansion Media (Catalog # CCM004). Filled histograms indicate cells stained with markers of undifferentiated MSCs including anti-CD44 (Catalog # AF6127) or CD45 (Catalog # MAB114). The open histograms show isotype-matched control staining. MSCs appropriately lack expression of CD45.

 

2006 Proposed Change to MSC Nomenclature

Although mesenchymal stromal cells were once referred to as ‘mesenchymal stem cells’, a change to ‘mesenchymal stromal cells’ was proposed by the International Society for Cellular Therapy in 2006.1

The change in nomenclature originates from two important factors:

  • Methods used to isolate mesenchymal stem cells yield a heterogeneous population of cells with only a fraction of these cells demonstrating multipotency.
  • The absence of direct evidence that mesenchymal stem cells can self-renew and differentiate in vivo.

Use of Mesenchymal Stem and Stromal Cell Terminology

Data supporting MSC self-renewal and multipotency have been obtained using in vitro conditions, which does not adequately reflect the in vivo environment. The lack of in vivo data has led some researchers to question the validity of the term ‘mesenchymal stem cell’ providing further support for the use of ‘mesenchymal stromal cells’ to describe MSCs.2 While ‘mesenchymal stromal cells’ may be the more scientifically accurate term for MSCs, the two terms are often used interchangeably in the literature. R&D Systems recognizes the use of both mesenchymal stem cells and mesenchymal stromal cells and uses ‘MSC’ to indicate mesenchymal stem/stromal cells to account for both designations.

Definitions of Mesenchymal Stromal Cells and Mesenchymal Stem Cells

  • Mesenchymal Stromal Cells – A heterogeneous population of cultured cells with similar characteristics such as the ability to adhere to plastic and the expression of specific marker proteins.
  • Mesenchymal Stem Cells – A subpopulation of mesenchymal stromal cells that have the capacity to self-renew and differentiate into mesodermal lineages when cultured in vitro. The capacity to self-renew and differentiate in vivo has yet to be clearly demonstrated for mesenchymal stem cells.

References

  • Dominici, M. et al. (2006) Cytotherapy 8:315.
  • Keating, A. (2012) Cell Stem Cell 10:709.

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Mesenchymal Stem Cells

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

Alternate Names
AEG1; LYRIC; LYRIC/3D3; Mesenchymal Stem Cells; metadherin

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, MSCs are cultured in expansion media using the following protocol:

  • Plate MSCs in expansion media
  • Culture MSCs to 80-90% confluency
  • Passage MSCs using fresh expansion media
 

 

Reagents Provided

Reagents supplied in the StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004):

  • 250 mL of MSC expansion media

 

Other Supplies Required

Reagents

  • Penicillin-Streptomycin (100X)
  • Trypsin-EDTA (10X)
  • Phosphate-Buffered Saline (PBS)

Materials

  • MSCs (e.g., Rat Mesenchymal Stem Cells, Catalog # PSC003)
  • 75 cm2 tissue culture flasks
  • 15 mL centrifuge tubes
  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 2 °C to 8 °C refrigerator
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be modified.

Culturing Mesenchymal Stem Cells

Add 3.5 - 4.0 x 105 MSCs resuspended in 20 mL of the pre-warmed StemXVivo® MSC Expansion Media to a T75 flask.

Culturing Mesenchymal Stem Cells

Replace the medium every 3 days with fresh StemXVivo MSC Expansion Media.

Culturing Mesenchymal Stem Cells

Culture the MSCs to 80-90% confluency.

Subculturing Mesenchymal Stem Cells

Remove and discard the media from the T75 tissue culture flask(s) containing the MSCs.

Wash the cells with PBS.

Subculturing Mesenchymal Stem Cells

Add 1-2 mL of pre-warmed trypsin to the MSCs and incubate the flask at 37 °C.

Subculturing Mesenchymal Stem Cells

Transfer the dissociated MSCs to a 15 mL conical tube.

Centrifuge at 400 x g for 5 minutes.

Subculturing Mesenchymal Stem Cells

Resuspend the cell pellet in 5 mL of StemXVivo® MSC Expansion Media.

Subculturing Mesenchymal Stem Cells

Perform a cell count.

Subculturing Mesenchymal Stem Cells

Add 3.5-4.0 x 105 MSCs resuspended in 20 mL of pre-warmed StemXVivo® MSC Expansion Media to each T75 flask.

Subculturing Mesenchymal Stem Cells

Citations for StemXVivo Mesenchymal Stem Cell Expansion Media

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. The Route by Which Intranasally Delivered Stem Cells Enter the Central Nervous System
    Authors: C Galeano, Z Qiu, A Mishra, SL Farnsworth, JJ Hemmi, A Moreira, P Edenhoffer, PJ Hornsby
    Cell Transplant, 2018;0(0):9636897187545.  2018
  2. SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix
    Authors: M Fukuda, T Yoshizawa, MF Karim, SU Sobuz, W Korogi, D Kobayasi, H Okanishi, M Tasaki, K Ono, T Sawa, Y Sato, M Chirifu, T Masuda, T Nakamura, H Tanoue, K Nakashima, Y Kobashigaw, H Morioka, E Bober, S Ohtsuki, Y Yamagata, Y Ando, Y Oike, N Araki, S Takeda, H Mizuta, K Yamagata
    Nat Commun, 2018;9(1):2833.  2018
  3. Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells
    Authors: R Fajka-Boja, A Marton, A Tóth, P Blazsó, V Tubak, B Bálint, I Nagy, Z Heged?s, C Vizler, RL Katona
    BMC Cancer, 2018;18(1):872.  2018
  4. Bile acids induce hepatic differentiation of mesenchymal stem cells.
    Authors: Sawitza I, Kordes C, Gotze S, Herebian D, Haussinger D
    Sci Rep, 2015;5(0):13320.  2015
  5. Intravenous and intratracheal mesenchymal stromal cell injection in a mouse model of pulmonary emphysema.
    Authors: Tibboel, Jeroen, Keijzer, Richard, Reiss, Irwin, de Jongste, Johan C, Post, Martin
    COPD, 2014;11(3):310-8.  2014
  6. Skin-derived mesenchymal stem cells help restore function to ovaries in a premature ovarian failure mouse model.
    Authors: Lai D, Wang F, Dong Z, Zhang Q
    PLoS ONE, 2014;9(5):e98749.  2014
  7. Dynamic microRNA profiles of hepatic differentiated human umbilical cord lining-derived mesenchymal stem cells.
    Authors: Cui, Lina, Zhou, Xinmin, Li, Jinge, Wang, Liuyi, Wang, Jingbo, Li, Qiang, Chu, Jindong, Zheng, Linhua, Wu, Qiong, Han, Zheyi, Shi, Yongquan, Han, Ying, Fan, Daiming
    PLoS ONE, 2012;7(9):e44737.  2012

FAQs

  1. Does StemXVivo® Mesenchymal Stem Cell Expansion Media need addition of serum before use?

    • The StemXVivo® Mesenchymal Stem Cell Expansion Media does not need addition of serum. Fetal bovine serum is included in the product, which is a complete medium and ready to use. The media may be supplemented with cytokine or growth factors for your desired cell culture application.

  2. Does StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004) contain Phenol Red?

    • Yes,StemXVivo® Mesenchymal Stem Cell Expansion Media contains Phenol Red.

  3. How long can Mesenchymal Stem Cells (MSCs) be cultured in StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004)?

    • MSCs can be grown to 80-90% confluency and subsequently subcultured using the protocol provided in the product datasheet. Researchers should establish the number of passages that is acceptable for their work. MSCs are sensitive to passsages and, if subcultured too many times, may start losing their MSC characteristics. Our MSC Functional Identification Kits can be used for validation of MSC mulitpotency.

  4. Can Mesenchymal Stem Cells (MSCs) grown in StemXVivo® MSC Expansion Media (Catalog # CCM004) be cryopreserved?

    • MSCs grown in StemXVivo® MSC Expansion Media can be cryopreserved using appropriate cryopreservation media, such as StemXVivo® Serum-Free MSC Freezing Media (Catalog # CCM016) or equivalent.

View all Cell Culture Product FAQs

Reviews for StemXVivo Mesenchymal Stem Cell Expansion Media

Average Rating: 4.5 (Based on 4 Reviews)

5 Star
75%
4 Star
0%
3 Star
25%
2 Star
0%
1 Star
0%

Have you used StemXVivo Mesenchymal Stem Cell Expansion Media?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


StemXVivo Mesenchymal Stem Cell Expansion Media
By Anonymous on 04/04/2018

StemXVivo Mesenchymal Stem Cell Expansion Media
By Anonymous on 03/23/2018

I am working on bone marrow stem cells.StemXVivo MSC expansion medium help to retain the population doubling time from P-4 to P-8 30 hrs to 34 hrs only, where when I culture these MSC in normal alpha MEM after some time cells do not divide/proliferate.


StemXVivo Mesenchymal Stem Cell Expansion Media
By Anonymous on 12/06/2016

StemXVivo Mesenchymal Stem Cell Expansion Media
By Anonymous on 12/06/2016

I used this media for my umbilical cord-derived MSC, after speaking with a representative, who gave me a sample. I tried the product because I saw my cells were growing really small in another brand product. After switching, the rate of growth of my cells dramatically increased, and I could not be happier than that!