SUCNR1/GPR91 Antibody - BSA Free

Western Blot: SUCNR1/GPR91 AntibodyBSA Free [NBP1-00861]

Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of human kidney tissue (A), mouse kidney tissue (B), rat kidney tissue (C), and hek293 cells (D) using GPR91 antibody at 2ug/ml.

Western Blot: SUCNR1/GPR91 AntibodyBSA Free [NBP1-00861]

Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Lane1:Hela whole cell lysate. Lane2:Mouse kidney tissue lysate. Lane3:Rat kidney tissue lysate.

Western Blot: SUCNR1/GPR91 AntibodyBSA Free [NBP1-00861]

Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of GPR91 (A135) antibody in extracts from HUVEC/MCF-7 cells.

Immunocytochemistry/ Immunofluorescence: SUCNR1/GPR91 Antibody - BSA Free [NBP1-00861]

Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - GPR91 antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.

Western Blot: SUCNR1/GPR91 Antibody - BSA Free [NBP1-00861] -

Western Blot: SUCNR1/GPR91 Antibody - BSA Free [NBP1-00861] - Succinate Signals via SUCNR1 in Mouse & Human NSCs(A–C) Representative confocal microscopy images of meningeal perivascular areas with transplanted fGFP+ iNSCs (A) & NSCs (B) expressing SUCNR1 in the brain of a mouse with EAE. The image in (C) shows transplanted SUCNR1+ iNSCs in close vicinity to SUCNR1+/F4/80+ MPs. Nuclei are stained with DAPI.(D) SUCNR1 protein expression relative to beta -tubulin in vitro. Data are shown as mean (±SEM) of n ≥ 3 independent replicates per condition.(E) Experimental setup for succinate treatment of iNSCs/NSCs in vitro.(F) Intracellular Ca2+ response after treatment with 500 μM succinate (live staining with Fluo-4AM). Representative images (baseline & during stimulation) are pseudocolored with red/blue according to high/low fluorescence intensity. Data are mean changes in fluorescence intensity as delta F/F0 (±SEM) from n ≥ 3 experiments.(G) Phospho-p38 MAPK (P-p38) & total p38 MAPK (p38) protein expression after succinate treatment. Data are P-p38/p38 expression relative to beta -tubulin & expressed as mean fold change (±SEM) versus untreated cells over n ≥ 3 independent experiments per condition.(H) qRT-PCR of SUCNR1 basal expression in human cells. Data are normalized on 18S & expressed as mean fold change (±SEM) versus NSCs from n ≥ 3 independent replicates per condition.(I) Representative blot of SUNCR1 basal protein expression in human cells.(J) P-p38 & p38 protein expression after stimulation with succinate ± pre-treatment with the irreversible inhibitor of the human SUCNR1 4c.The scale bars represent 25 μm. ∗p ≤ 0.05 versus 0’. hBJFs, human BJ fibroblasts; ND, not detected. See also Figure S4. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29478844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SUCNR1/GPR91 Antibody - BSA Free [NBP1-00861] -

Western Blot: SUCNR1/GPR91 Antibody - BSA Free [NBP1-00861] - Succinate Signals via SUCNR1 in Mouse & Human NSCs(A–C) Representative confocal microscopy images of meningeal perivascular areas with transplanted fGFP+ iNSCs (A) & NSCs (B) expressing SUCNR1 in the brain of a mouse with EAE. The image in (C) shows transplanted SUCNR1+ iNSCs in close vicinity to SUCNR1+/F4/80+ MPs. Nuclei are stained with DAPI.(D) SUCNR1 protein expression relative to beta -tubulin in vitro. Data are shown as mean (±SEM) of n ≥ 3 independent replicates per condition.(E) Experimental setup for succinate treatment of iNSCs/NSCs in vitro.(F) Intracellular Ca2+ response after treatment with 500 μM succinate (live staining with Fluo-4AM). Representative images (baseline & during stimulation) are pseudocolored with red/blue according to high/low fluorescence intensity. Data are mean changes in fluorescence intensity as delta F/F0 (±SEM) from n ≥ 3 experiments.(G) Phospho-p38 MAPK (P-p38) & total p38 MAPK (p38) protein expression after succinate treatment. Data are P-p38/p38 expression relative to beta -tubulin & expressed as mean fold change (±SEM) versus untreated cells over n ≥ 3 independent experiments per condition.(H) qRT-PCR of SUCNR1 basal expression in human cells. Data are normalized on 18S & expressed as mean fold change (±SEM) versus NSCs from n ≥ 3 independent replicates per condition.(I) Representative blot of SUNCR1 basal protein expression in human cells.(J) P-p38 & p38 protein expression after stimulation with succinate ± pre-treatment with the irreversible inhibitor of the human SUCNR1 4c.The scale bars represent 25 μm. ∗p ≤ 0.05 versus 0’. hBJFs, human BJ fibroblasts; ND, not detected. See also Figure S4. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29478844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: SUCNR1/GPR91 Antibody - BSA Free [NBP1-00861]

Analysis of a FFPE tissue section of human kidney using 1:200 dilution of SUCNR1/GPR91 Antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.