Q: Does this product work on endometrial tissue?

A: Syndecan-1/CD138 antibody (NB100-64980) is a great antibody and it should work in any sections as long as they have CD138 antigen. As far as endometrial tissue is concerned, I am assuming that the cancerous endometrial tissue express Syndecan-1/CD138 while the normal tissue does not express it that much. And we would suggest the customer to include a positive control section in the assay using any of the following tissues: Normal Tissues:- Placenta, Colon, Small Intestine or Tonsil; Cancerous Tissues:- Ovarian cancer, rectal cancer, Lung cancer.

Q: Is this differ from anti human syndecan-1 mouse B-B4 monoclonal antibody?

A: Our NB100-64980 is Syndecan-1/CD138 antibody’s clone B-A38 and this clone is similar to clone B-B4. Both of these clones are IgG1 monoclonal antibodies generated in mouse using U266 myeloma cell line as immunogen. Both of these clones recognize CD138 antigen/ Syndecan-1 protein selectively on Plasma cells /B Cells and are routinely utilized for the diagnosis of multiple myeloma. We do not have license to sell Clone B-B4 at the moment, therefore, the customer can buy our Syndecan-1/CD138 antibody (clone B-A38) NB100-64980 which is a similar product by nature and performance in immunoassays.

Syndecan-1/CD138 Antibody (B-A38)

Novus Biologicals | Catalog # NB100-64980

(1)

Citations (16)

Product Documents

Clone B-A38 was used by HLDA to establish CD designation.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Functional

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # B-A38

View all Syndecan-1/CD138 Primary Antibodies »

Product Specifications

Immunogen

U266 cell line

Localization

Membrane; Single-pass type I membrane protein.

Specificity

NB100-64980 recognizes a heparan sulfate rich membrane glycoprotein of 200kD known as Syndecan-1 (CD138). Syndecan functions as a receptor for collagen, fibronectin and thrombospondin. NB100-64980 stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Syndecan-1/CD138 Antibody (B-A38)

Flow Cytometry: Syndecan-1/CD138 Antibody (B-A38) [NB100-64980]

Flow Cytometry: Syndecan-1/CD138 Antibody (B-A38) [NB100-64980] - Staining of U266 cell line with MOUSE ANTI HUMAN CD138: FITC.

Immunohistochemistry-Parafin: Mouse Monoclonal Syndecan-1/CD138 Antibody (B-A38) [NB100-64980] -

Immunohistochemistry-Parafin: Mouse Monoclonal Syndecan-1/CD138 Antibody (B-A38) [NB100-64980] - Syndecan-1/CD138 analysis on human lymph node tissue. Heat induced epitope retrieval-basic. Primary antibody x500 (0.2ug/mL). Image from verified customer review.

Applications for Syndecan-1/CD138 Antibody (B-A38)

Application

Recommended Usage

Flow Cytometry

Neat

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:100-1:2000

Application Notes

For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC: This product requires heat pre-treatment. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Reviewed Applications

Read 1 review rated 5 using NB100-64980 in the following applications:

Immunohistochemistry-Paraffin (1 Review)

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Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS, 1% Bovine Serum Albumin

Preservative

0.09% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Syndecan-1/CD138

CD138 / Syndecan 1 is a transmembrane heparin sulphate proteoglycan which is made up of one core protein and five glycosaminoglycan. CD138 is expected to play a role in cell adhesion. It is expressed on the surface of pre B cells and plasma cells but is absent from mature B cells. It is a selective marker for B cell lymphoblastic leukemia and lymphoplasmocytoid leukemia. It is lost from the apoptotic myeloma cells; hence is a useful marker for viable myeloma cells. This antibody stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology.

Alternate Names

CD138, SDC1, Syndecan1

Entrez Gene IDs

6382 (Human)

Gene Symbol

SDC1

UniProt

P18827 (Human)

Additional Syndecan-1/CD138 Products

Product Documents for Syndecan-1/CD138 Antibody (B-A38)

Download Product Datasheets

Download MSDS NB100-64980

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Syndecan-1/CD138 Antibody (B-A38)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Cancer Stem Cell Markers

Citations for Syndecan-1/CD138 Antibody (B-A38)

Customer Reviews for Syndecan-1/CD138 Antibody (B-A38) (1)

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1 Customer Rating

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Syndecan-1/CD138 Antibody (B-A38)

Name: Anonymous

Application: Immunohistochemistry-Paraffin

Sample Tested: Human lymph node

Species: Human

Verified Customer | Posted 09/14/2023

plasmacytes were distinctly CD138-positive

heat induced epitope retrieval-basic primary antibody x500 (0.2ug/mL) BOND refine kit

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Protocols

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FAQs for Syndecan-1/CD138 Antibody (B-A38)

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Q: Does this product work on endometrial tissue?

A: Syndecan-1/CD138 antibody (NB100-64980) is a great antibody and it should work in any sections as long as they have CD138 antigen. As far as endometrial tissue is concerned, I am assuming that the cancerous endometrial tissue express Syndecan-1/CD138 while the normal tissue does not express it that much. And we would suggest the customer to include a positive control section in the assay using any of the following tissues: Normal Tissues:- Placenta, Colon, Small Intestine or Tonsil; Cancerous Tissues:- Ovarian cancer, rectal cancer, Lung cancer.
Q: Is this differ from anti human syndecan-1 mouse B-B4 monoclonal antibody?

A: Our NB100-64980 is Syndecan-1/CD138 antibody’s clone B-A38 and this clone is similar to clone B-B4. Both of these clones are IgG1 monoclonal antibodies generated in mouse using U266 myeloma cell line as immunogen. Both of these clones recognize CD138 antigen/ Syndecan-1 protein selectively on Plasma cells /B Cells and are routinely utilized for the diagnosis of multiple myeloma. We do not have license to sell Clone B-B4 at the moment, therefore, the customer can buy our Syndecan-1/CD138 antibody (clone B-A38) NB100-64980 which is a similar product by nature and performance in immunoassays.
Q: Does this product work on endometrial tissue?

A: Syndecan-1/CD138 antibody (NB100-64980) is a great antibody and it should work in any sections as long as they have CD138 antigen. As far as endometrial tissue is concerned, I am assuming that the cancerous endometrial tissue express Syndecan-1/CD138 while the normal tissue does not express it that much. And we would suggest the customer to include a positive control section in the assay using any of the following tissues: Normal Tissues:- Placenta, Colon, Small Intestine or Tonsil; Cancerous Tissues:- Ovarian cancer, rectal cancer, Lung cancer.
Q: Is this differ from anti human syndecan-1 mouse B-B4 monoclonal antibody?

A: Our NB100-64980 is Syndecan-1/CD138 antibody’s clone B-A38 and this clone is similar to clone B-B4. Both of these clones are IgG1 monoclonal antibodies generated in mouse using U266 myeloma cell line as immunogen. Both of these clones recognize CD138 antigen/ Syndecan-1 protein selectively on Plasma cells /B Cells and are routinely utilized for the diagnosis of multiple myeloma. We do not have license to sell Clone B-B4 at the moment, therefore, the customer can buy our Syndecan-1/CD138 antibody (clone B-A38) NB100-64980 which is a similar product by nature and performance in immunoassays.