TAB1 Antibody (2A12) - Azide and BSA Free

Novus Biologicals | Catalog # H00010454-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A12

Format

Azide and BSA Free
View all TAB1 Primary Antibodies »

Product Specifications

Immunogen

MAP3K7IP1 (NP_705717.1, 3 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AQRRSLLQSEQQPSWTDDLPLCHLSGVGSASNRSYSADGKGTESHPPEDSWLKFRSENNCFLYGVFNGYDGNRVTNFVAQRLSAELLLGQLNAEHAEA

Specificity

MAP3K7IP1 (2A12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TAB1 Antibody (2A12) - Azide and BSA Free

Western Blot: TAB1 Antibody (2A12) [H00010454-M03]

Western Blot: TAB1 Antibody (2A12) [H00010454-M03]

Western Blot: TAB1 Antibody (2A12) [H00010454-M03] - Analysis of MAP3K7IP1 expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: TAB1 Antibody (2A12) [H00010454-M03]

Immunocytochemistry/ Immunofluorescence: TAB1 Antibody (2A12) [H00010454-M03]

Immunocytochemistry/Immunofluorescence: TAB1 Antibody (2A12) [H00010454-M03] - Analysis of monoclonal antibody to MAP3K7IP1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: TAB1 Antibody (2A12) [H00010454-M03]

Western Blot: TAB1 Antibody (2A12) [H00010454-M03]

Western Blot: TAB1 Antibody (2A12) [H00010454-M03] - Analysis of MAP3K7IP1 expression in PC-12 (Cat # L012V1).
ELISA: TAB1 Antibody (2A12) [H00010454-M03]

ELISA: TAB1 Antibody (2A12) [H00010454-M03]

ELISA: TAB1 Antibody (2A12) [H00010454-M03] - Detection limit for recombinant GST tagged MAP3K7IP1 is approximately 0.3ng/ml as a capture antibody.
Proximity Ligation Assay: TAB1 Antibody (2A12) [H00010454-M03]

Proximity Ligation Assay: TAB1 Antibody (2A12) [H00010454-M03]

Proximity Ligation Assay: TAB1 Antibody (2A12) [H00010454-M03] - Analysis of protein-protein interactions between HSPA1L and MAP3K7IP1 HeLa cells were stained with anti-HSPA1L rabbit purified polyclonal 1:1200 and anti-MAP3K7IP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for TAB1 Antibody (2A12) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TAB1

The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Long Name

TAK1-binding Protein

Alternate Names

MAP3K7IP1

Entrez Gene IDs

10454 (Human)

Gene Symbol

TAB1

OMIM

602615 (Human)

UniProt

NP_705717 (Human)

Additional TAB1 Products

Product Documents for TAB1 Antibody (2A12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TAB1 Antibody (2A12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TAB1 Antibody (2A12) - Azide and BSA Free

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  • Q: Why is there double band in the blot using TAB1(2A12) antibody?

    A: Human TAB1 has two isoforms with predicted molecular weights of 54.6 kDa and 49.9 kDa. And the immunogen of H00010454-M03 has 100% similarity with the sequences of both isoforms of Human TAB1. Therefore, we believe that the cause of double band seen in the validation image of H00010454-M03 is related to two isoforms of TAB1 protein existing in HeLa cell lysate.

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