TACS MTT Cell Proliferation Assay
TACS MTT Cell Proliferation Assay SummaryA sensitive kit for the measurement of cell proliferation based upon the reduction of the tetrazolium salt, 3,[4,5-dimethylthiazol-2- yl]-2,5-diphenyl-tetrazolium bromide (MTT).
• Allows measurement of proliferation rate, and conversely, the reduction in cell viability.
• Minimizes the number of steps necessary to complete the assay and interpret the data.
• Yields low background absorbance values in the absence of cells.
• Accurate quantification of changes in cell proliferation due to the linear relationship between cell number and signal produced for each cell type.
• Stable when stored at 4°C.
Why Use the TACS MTT Cell Proliferation Assay?
Changes in cell proliferative activity caused by trophic factors, growth inhibitors, or inducers and inhibitors of apoptosis, may be quantified using the TACS MTT Cell Proliferation Assay. MTT is reduced to an insoluble formazan dye by mitochondrial enzymes associated with metabolic activity. The reduction of MTT is primarily due to glycolytic activity within the cell and is dependent upon the presence of NADH and NADPH. The resultant intracellular purple formazan can be solubilized and quantitated by spectroscopic means.
MTT can be used to safely assess cell proliferation, cell viability, and/or cytotoxicity. MTT is added directly to the culture medium and is reduced by metabolically active cells to insoluble purple formazan dye crystals. The absorbance of the sample is read directly in the wells at an optimal wavelength of 570 nm, but any filter that absorbs between 550 and 600 nm may be used.
• MTT Reagent
• Detergent Reagent
For research use only. Not for diagnostic use.
Complete Your Research
MTT Assay of Human Tissue-derived Liver Organoids. Human Liver organoids were derived from human biopsy tissue. Undifferentiated liver organoids were formed by embedding dissociated tissue in Cultrex RGF BME, Type 2 (Catalog # 3533-005-02) and culturing in specialized media. Liver organoids were differentiated using media containing Recombinant Human FGF-19 (Catalog # 969-FG), DAPT (Catalog # 2634), and Dexamethasone (Catalog # 1126). A) Representative images of untreated control and DMSO-treated (positive control) undifferentiated and differentiated liver organoids. B) Quantification of MTT staining of cells treated with varying doses of acetaminophen, Trovafloxin, or Doxorubicin showed a dose-dependent loss of cell viability under each condition. Interestingly, liver organoids showed increased vulnerability to hepatotoxicity as they matured in differentiation media.
Toxicity analysis of iPSC-derived Hepatocytes using TACS MTT Proliferation Assay and the CometChip Assay. iPSC-derived hepatocytes were generated using the StemXVivo Hepatocyte Differentiation Kit (Catalog # SC033). iPSC-derived hepatocytes were treated with Acetaminophen, Trovafloxacin, and Doxorubicin and dose response toxicity profiles using the (A) TACS MTT Cell Proliferation Assay or (B) CometChip (Catalog # 4260-096-ESK). Using the MTT assay, Trovafloxacin and Doxorubicin showed significantly increased loss of cell metabolic activity at 100 µM and >10 µM, respectively. Increased DNA damage was detected at these two concentrations using the CometChip (n ≥ 3 for each group).
HepG2 Spheroid Toxicity Detected using the MTT Assay. A) Hepatocyte spheroids were formed by plating HepG2 liver hepatocellular carcinoma cells (10,000 cells per well) in a 24-well plate coated with Cultrex RGF BME, Type 2 (Catalog # 3533-005-02). B) HepG2 spheroids were cultured for 21 days prior exposure to acetaminophen, trovafloxacin, or doxorubicin. Spheroids showed significantly increased loss of cell metabolic activity at 100 µM and >10 µM, respectively (n ≥ 3 for each group), as detected by the MTT Assay.
Citations for TACS MTT Cell Proliferation Assay
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miR766-3p and miR124-3p Dictate Drug Resistance and Clinical Outcome in HNSCC
Authors: T Shibata, DY Cao, TB Dar, F Ahmed, SA Bhat, LC Veiras, EA Bernstein, AA Khan, M Chaum, SL Shiao, WG Tourtellot, JF Giani, KE Bernstein, X Cui, E Vail, Z Khan
Cancers, 2022;14(21):. 2022
Acidic ascites inhibits ovarian cancer cell proliferation and correlates with the metabolomic, lipidomic and inflammatory phenotype of human patients
Authors: Q Yang, G Bae, G Nadiradze, A Castagna, G Berezhnoy, L Zizmare, A Kulkarni, Y Singh, FJ Weinreich, S Kommoss, MA Reymond, C Trautwein
Journal of Translational Medicine, 2022;20(1):581. 2022
Toll-like receptor 4 activation enhances Orai1-mediated calcium signal promoting cytokine production in spinal astrocytes
Authors: H Birla, J Xia, X Gao, H Zhao, F Wang, S Patel, A Amponsah, A Bekker, YX Tao, H Hu
Cell Calcium, 2022;105(0):102619. 2022
Enhancement of MDM2 inhibitory effects through blocking nuclear export mechanisms in ovarian cancer cells
Authors: A Alzahrani, U Natarajan, A Rathinavel
Oncogene, 2022;266(0):57-68. 2022
Targeted Extracellular Vesicles Delivered Verrucarin A to Treat Glioblastoma
Authors: K Chen, Y Si, JS Guan, Z Zhou, S Kim, T Kim, L Shan, CD Willey, L Zhou, X Liu
Biomedicines, 2022;10(1):. 2022
Mutant p53 promotes RCP-dependent chemoresistance coinciding with increased delivery of P-glycoprotein to the plasma membrane
Authors: V Phatak, Y von Grabow, J Janus, L Officer, C Behan, L Aschauer, L Pinon, H Mackay, S Zanivan, JC Norman, M Kelly, J Le Quesne, PAJ Muller
Cell Death & Disease, 2021;12(2):207. 2021
Hepatitis B virus induces sorafenib resistance in liver cancer via upregulation of cIAP2 expression
Authors: S Zhang, N Li, Y Sheng, W Chen, Q Ma, X Yu, J Lian, J Zeng, Y Yang, J Yan
Infectious agents and cancer, 2021;16(1):20. 2021
Cell-autonomous inflammation of BRCA1-deficient ovarian cancers drives both tumor-intrinsic immunoreactivity and immune resistance via STING
Authors: M Bruand, D Barras, M Mina, E Ghisoni, M Morotti, E Lanitis, N Fahr, M Desbuisson, A Grimm, H Zhang, C Chong, J Dagher, S Chee, T Tsianou, J Dorier, BJ Stevenson, C Iseli, C Ronet, S Bobisse, R Genolet, J Walton, M Bassani-St, LE Kandalaft, B Ren, I McNeish, E Swisher, A Harari, M Delorenzi, G Ciriello, M Irving, S Rusakiewic, PG Foukas, F Martinon, D Dangaj Lan, G Coukos
Cell Reports, 2021;36(3):109412. 2021
Pancreastatin Reduces Alternatively Activated Macrophages, Disrupts the Epithelial Homeostasis and Aggravates Colonic Inflammation. A Descriptive Analysis
Authors: N Eissa, O Elgazzar, H Hussein, GN Hendy, CN Bernstein, JE Ghia
Biomedicines, 2021;9(2):. 2021
XMD-17-51 Inhibits DCLK1 Kinase and Prevents Lung Cancer Progression
Authors: WQ Yang, WJ Zhao, LL Zhu, SJ Xu, XL Zhang, Y Liang, XF Ding, A Kiselyov, G Chen
Frontiers in Pharmacology, 2021;12(0):603453. 2021
Differential Expression of PD-L1 during Cell Cycle Progression of Head and Neck Squamous Cell Carcinoma
Authors: D Schulz, M Wetzel, J Eichberger, G Piendl, G Brockhoff, AK Wege, TE Reichert, T Ettl, RJ Bauer
International Journal of Molecular Sciences, 2021;22(23):. 2021
Ruxolitinib Combined with Gemcitabine against Cholangiocarcinoma Growth via the JAK2/STAT1/3/ALDH1A3 Pathway
Authors: SY Chung, YP Hung, YR Pan, YC Chang, CE Wu, DS Hsu, PM Chang, ML Lu, CF Huang, Y Su, M Hsiao, CN Yeh, MH Chen
Biomedicines, 2021;9(8):. 2021
The Effect of Neddylation Inhibition on Inflammation-Induced MMP9 Gene Expression in Esophageal Squamous Cell Carcinoma
Authors: A Hryniewicz, J Wierzbicki, R Tabola, K Stach, K Sossey-Ala, K Augoff
International Journal of Molecular Sciences, 2021;22(4):. 2021
Chemical analysis and anticancer activity of sesterterpenoid from an endophytic fungus Hypomontagnella monticulosa Zg15SU and its host Zingiber griffithii Baker
Authors: A Lutfia, E Munir, Y Yurnaliza, M Basyuni
Heliyon, 2021;7(2):e06292. 2021
Synergisms of genome and metabolism stabilizing antitumor therapy (GMSAT) in human breast and colon cancer cell lines: a novel approach to screen for synergism
Authors: J Ruhnau, J Parczyk, K Danker, B Eickholt, A Klein
BMC Cancer, 2020;20(1):617. 2020
SOX9 Knockout Induces Polyploidy and Changes Sensitivity to Tumor Treatment Strategies in a Chondrosarcoma Cell Line
Authors: S Stöckl, G Lindner, S Li, P Schuster, S Haferkamp, F Wagner, PM Prodinger, G Multhoff, M Boxberg, A Hillmann, RJ Bauer, S Grässel
Int J Mol Sci, 2020;21(20):. 2020
Targeting the ABC transporter ABCB5 sensitizes glioblastoma to temozolomide-induced apoptosis through a cell-cycle checkpoint regulation mechanism
Authors: CAA Lee, P Banerjee, BJ Wilson, S Wu, Q Guo, G Berg, S Karpova, A Mishra, JW Lian, J Tran, M Emmerich, GF Murphy, MH Frank, NY Frank
J. Biol. Chem., 2020;0(0):. 2020
Methodology for processing mastectomy and cryopreservation of breast cancer tissue in a resource- poor setting: A pilot study
Authors: UA Okoli, MT Okafor, KA Agu, AC Ndubuisi, IJ Nwigwe, EO Nna, OC Okafor, FI Ukekwe, TU Nwagha, VC Menkiti, CO Eze, KC Onyekwelu, JE Ikekpeazu, CA Anusiem, AU Mbah, CP Chijioke, IJ Udeniya
Cryobiology, 2020;0(0):. 2020
Direct incorporation of mesenchymal stem cells into a Nanofiber scaffold - in vitro and in vivo analysis
Authors: KF Schüttler, MW Bauhofer, V Ketter, K Giese, DA Eschbach, M Yenigün, S Fuchs-Wink, JRJ Paletta
Sci Rep, 2020;10(1):9557. 2020
l-tetrahydropalmatine suppresses osteoclastogenesis in vivo and in vitro via blocking RANK-TRAF6 interactions and inhibiting NF-kappaB and MAPK pathways
Authors: X Zhi, L Wang, H Chen, C Fang, J Cui, Y Hu, L Cao, W Weng, Q Zhou, L Qin, H Song, Y Wang, Y Wang, H Jiang, X Li, S Wang, X Chen, J Su
J. Cell. Mol. Med., 2019;0(0):. 2019
Anticancer effect of fucoidan on cell proliferation, cell cycle progression, genetic damage and apoptotic cell death in HepG2 cancer cells
Authors: A P, A K, S L, M M, M K
Toxicol Rep, 2019;6(0):556-563. 2019
Circular RNA circ-FoxO3 Inhibits Myoblast Cells Differentiation
Authors: X Li, C Li, Z Liu, W Ni, R Yao, Y Xu, R Quan, M Zhang, H Li, L Liu, S Hu
Cells, 2019;8(6):. 2019
ALDH1A3, the major aldehyde dehydrogenase isoform in human cholangiocarcinoma cells, affects prognosis and gemcitabine resistance in cholangiocarcinoma patients
Authors: MH Chen, JJ Weng, CT Cheng, RC Wu, SC Huang, CE Wu, YH Chung, CY Liu, MH Chang, MH Chen, KC Chiang, TS Yeh, Y Su, CN Yeh
Clin Cancer Res, 2016;0(0):. 2016
Inhibition of hyaluronic acid formation sensitizes chronic myelogenous leukemia to treatment with doxorubicin
Authors: Olga N Uchakina
Glycobiology, 2016;0(0):. 2016
Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
Authors: Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA
Blood, 2007;109(4):1479-89. 2007
Suppression of Mycobacterium tuberculosis induced reactive oxygen species (ROS) and TNF-alpha mRNA expression in human monocytes by allicin.
Authors: Hasan N, Yusuf N, Toossi Z, Islam N
FEBS Lett., 2006;580(10):2517-22. 2006
Nicotine Reduces Survival via Augmentation of Paracrine HGF-MET Signaling in the Pancreatic Cancer Microenvironment.
Authors: Delitto D, Zhang D, Han S, Black B, Knowlton A, Vlada A, Sarosi G, Behrns K, Thomas R, Lu X, Liu C, George T, Hughes S, Wallet S, Trevino J
Clin Cancer Res, 0;22(7):1787-99. 0
In vitro antimicrobial studies of silver carbene complexes: activity of free and nanoparticle carbene formulations against clinical isolates of pathogenic bacteria.
Authors: Leid J, Ditto A, Knapp A, Shah P, Wright B, Blust R, Christensen L, Clemons C, Wilber J, Young G, Kang A, Panzner M, Cannon C, Yun Y, Youngs W, Seckinger N, Cope E
J Antimicrob Chemother, 0;67(1):138-48. 0
Identification of Chemical Compounds That Inhibit Protein Synthesis in Pseudomonas aeruginosa.
Authors: Palmer S, Hu Y, Keniry M, Bullard J
SLAS Discov, 0;22(6):775-782. 0
Comparison of electrospun and solvent cast polylactic acid (PLA)/poly(vinyl alcohol) (PVA) inserts as potential ocular drug delivery vehicles.
Authors: Bhattarai R, Das A, Alzhrani R, Kang D, Bhaduri S, Boddu S
Mater Sci Eng C Mater Biol Appl, 0;77(0):895-903. 0
Lenalidomide interferes with tumor-promoting properties of nurse-like cells in chronic lymphocytic leukemia.
Authors: Fiorcari S, Martinelli S, Bulgarelli J, Audrito V, Zucchini P, Colaci E, Potenza L, Narni F, Luppi M, Deaglio S, Marasca R, Maffei R
Haematologica, 0;100(2):253-62. 0
Development and qualification of an LC-MS/MS method for investigating the biological implications of micelle entrapped paclitaxel in cell culture and rats.
Authors: Kaddoumi A, Gill K, Elfakhri K, Nazzal S
Biomed Chromatogr, 0;31(9):. 0
A functional screening of the kinome identifies the Polo-like kinase 4 as a potential therapeutic target for malignant rhabdoid tumors, and possibly, other embryonal tumors of the brain.
Authors: Sredni S, Suzuki M, Yang J, Topczewski J, Bailey A, Gokirmak T, Gross J, de Andrade A, Kondo A, Piper D, Tomita T
Pediatr Blood Cancer, 0;64(11):. 0
Vaccine-induced tumor necrosis factor-producing T cells synergize with cisplatin to promote tumor cell death.
Authors: van der Sluis T, van Duikeren S, Huppelschoten S, Jordanova E, Beyranvand Nejad E, Sloots A, Boon L, Smit V, Welters M, Ossendorp F, van de Water B, Arens R, van der Burg S, Melief C
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In vitro cytotoxicity assessment of a West Virginia chemical spill mixture involving 4-methylcyclohexanemethanol and propylene glycol phenyl ether.
Authors: Han A, Fabyanic E, Miller J, Prediger M, Prince N, Mouch J, Boyd J
Environ Monit Assess, 0;189(4):190. 0
Identification of a negative regulatory role for spi-C in the murine B cell lineage.
Authors: Li S, Solomon L, Fulkerson P, DeKoter R
J Immunol, 0;194(8):3798-807. 0
Integrative genomics identified RFC3 as an amplified candidate oncogene in esophageal adenocarcinoma.
Authors: Lockwood W, Thu K, Lin L, Pikor L, Chari R, Lam W, Beer D
Clin Cancer Res, 0;18(7):1936-46. 0
A Gene Expression Signature Associated with Overall Survival in Patients with Hepatocellular Carcinoma Suggests a New Treatment Strategy.
Authors: Gillet J, Andersen J, Madigan J, Varma S, Bagni R, Powell K, Burgan W, Wu C, Calcagno A, Ambudkar S, Thorgeirsson S, Gottesman M
Mol Pharmacol, 0;89(2):263-72. 0
Regulation of bovine pyruvate carboxylase mRNA and promoter expression by thermal stress.
Authors: White H, Koser S, Donkin S
J Anim Sci, 0;90(9):2979-87. 0
Prothymosin-alpha interacts with mutant huntingtin and suppresses its cytotoxicity in cell culture.
Authors: Dong G, Callegari E, Gloeckner C, Ueffing M, Wang H
J Biol Chem, 0;287(2):1279-89. 0
Synergistic effect of Ebselen and gamma radiation on breast cancer cells.
Authors: Thabet N, Moustafa E
Int J Radiat Biol, 0;93(8):784-792. 0
Identification of Chemical Compounds That Inhibit the Function of Glutamyl-tRNA Synthetase from Pseudomonas aeruginosa.
Authors: Hu Y, Guerrero E, Keniry M, Manrrique J, Bullard J
J Biomol Screen, 0;20(9):1160-70. 0
Anticancer and Anti-Inflammatory Properties of Ganoderma lucidum Extract Effects on Melanoma and Triple-Negative Breast Cancer Treatment.
Authors: Barbieri A, Quagliariello V, Del Vecchio V, Falco M, Luciano A, Amruthraj N, Nasti G, Ottaiano A, Berretta M, Iaffaioli R, Arra C
Nutrients, 0;9(3):. 0
An easy method for bacterial expression and purification of wild-type and mutant superoxide dismutase 1 (SOD1).
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Characterization of CM572, a Selective Irreversible Partial Agonist of the Sigma-2 Receptor with Antitumor Activity.
Authors: Nicholson H, Comeau A, Mesangeau C, McCurdy C, Bowen W
J Pharmacol Exp Ther, 0;354(2):203-12. 0
Imidazolium salts as small-molecule urinary bladder exfoliants in a murine model.
Authors: Wagers P, Tiemann K, Shelton K, Kofron W, Panzner M, Wooley K, Youngs W, Hunstad D
Antimicrob Agents Chemother, 0;59(9):5494-502. 0
Can mesenchymal stem cells pretreated with platelet-rich plasma modulate tissue remodeling in a rat with burned skin?
Authors: Hosni Ahmed H, Rashed L, Mahfouz S, Elsayed Hussein R, Alkaffas M, Mostafa S, Abusree A
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