TACS MTT Cell Proliferation Assay

A sensitive kit for the measurement of cell proliferation using MTT
Catalog # Availability Size / Price Qty
4890-025-K
4890-050-K
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TACS MTT Cell Proliferation Assay Summary

A sensitive kit for the measurement of cell proliferation based upon the reduction of the tetrazolium salt, 3,[4,5-dimethylthiazol-2- yl]-2,5-diphenyl-tetrazolium bromide (MTT).

Key Benefits

• Allows measurement of proliferation rate, and conversely, the reduction in cell viability.
• Minimizes the number of steps necessary to complete the assay and interpret the data.
• Yields low background absorbance values in the absence of cells.
• Accurate quantification of changes in cell proliferation due to the linear relationship between cell number and signal produced for each cell type.
• Stable when stored at 4°C.

Why Use the TACS MTT Cell Proliferation Assay?

Changes in cell proliferative activity caused by trophic factors, growth inhibitors, or inducers and inhibitors of apoptosis, may be quantified using the TACS MTT Cell Proliferation Assay. MTT is reduced to an insoluble formazan dye by mitochondrial enzymes associated with metabolic activity. The reduction of MTT is primarily due to glycolytic activity within the cell and is dependent upon the presence of NADH and NADPH. The resultant intracellular purple formazan can be solubilized and quantitated by spectroscopic means.

MTT can be used to safely assess cell proliferation, cell viability, and/or cytotoxicity. MTT is added directly to the culture medium and is reduced by metabolically active cells to insoluble purple formazan dye crystals. The absorbance of the sample is read directly in the wells at an optimal wavelength of 570 nm, but any filter that absorbs between 550 and 600 nm may be used.

Kit Contents

• MTT Reagent
• Detergent Reagent

Kit Contents

  • MTT Reagent
  • Detergent Reagent

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

FAQs

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