TACS MTT Cell Proliferation Assay
TACS MTT Cell Proliferation Assay Summary
• Allows measurement of proliferation rate, and conversely, the reduction in cell viability.
• Minimizes the number of steps necessary to complete the assay and interpret the data.
• Yields low background absorbance values in the absence of cells.
• Accurate quantification of changes in cell proliferation due to the linear relationship between cell number and signal produced for each cell type.
• Stable when stored at 4°C.
Why Use the TACS MTT Cell Proliferation Assay?
Changes in cell proliferative activity caused by trophic factors, growth inhibitors, or inducers and inhibitors of apoptosis, may be quantified using the TACS MTT Cell Proliferation Assay. MTT is reduced to an insoluble formazan dye by mitochondrial enzymes associated with metabolic activity. The reduction of MTT is primarily due to glycolytic activity within the cell and is dependent upon the presence of NADH and NADPH. The resultant intracellular purple formazan can be solubilized and quantitated by spectroscopic means.
MTT can be used to safely assess cell proliferation, cell viability, and/or cytotoxicity. MTT is added directly to the culture medium and is reduced by metabolically active cells to insoluble purple formazan dye crystals. The absorbance of the sample is read directly in the wells at an optimal wavelength of 570 nm, but any filter that absorbs between 550 and 600 nm may be used.
• MTT Reagent
• Detergent Reagent
For research use only. Not for diagnositic use.
MTT Assay of Human Tissue-derived Liver Organoids. Human Liver organoids were derived from human biopsy tissue. Undifferentiated liver organoids were formed by embedding dissociated tissue in Cultrex RGF BME, Type 2 (Catalog # 3533-005-02) and culturing in specialized media. Liver organoids were differentiated using media containing Recombinant Human FGF-19 (Catalog # 969-FG), DAPT (Catalog # 2634), and Dexamethasone (Catalog # 1126). A) Representative images of untreated control and DMSO-treated (positive control) undifferentiated and differentiated liver organoids. B) Quantification of MTT staining of cells treated with varying doses of acetaminophen, Trovafloxin, or Doxorubicin showed a dose-dependent loss of cell viability under each condition. Interestingly, liver organoids showed increased vulnerability to hepatotoxicity as they matured in differentiation media.
Toxicity analysis of iPSC-derived Hepatocytes using TACS MTT Proliferation Assay and the CometChip Assay. iPSC-derived hepatocytes were generated using the StemXVivo Hepatocyte Differentiation Kit (Catalog # SC033). iPSC-derived hepatocytes were treated with Acetaminophen, Trovafloxacin, and Doxorubicin and dose response toxicity profiles using the (A) TACS MTT Cell Proliferation Assay or (B) CometChip (Catalog # 4260-096-ESK). Using the MTT assay, Trovafloxacin and Doxorubicin showed significantly increased loss of cell metabolic activity at 100 µM and >10 µM, respectively. Increased DNA damage was detected at these two concentrations using the CometChip (n ≥ 3 for each group).
HepG2 Spheroid Toxicity Detected using the MTT Assay. A) Hepatocyte spheroids were formed by plating HepG2 liver hepatocellular carcinoma cells (10,000 cells per well) in a 24-well plate coated with Cultrex RGF BME, Type 2 (Catalog # 3533-005-02). B) HepG2 spheroids were cultured for 21 days prior exposure to acetaminophen, trovafloxacin, or doxorubicin. Spheroids showed significantly increased loss of cell metabolic activity at 100 µM and >10 µM, respectively (n ≥ 3 for each group), as detected by the MTT Assay.
Citations for TACS MTT Cell Proliferation Assay
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Pancreastatin Reduces Alternatively Activated Macrophages, Disrupts the Epithelial Homeostasis and Aggravates Colonic Inflammation. A Descriptive Analysis
Authors: N Eissa, O Elgazzar, H Hussein, GN Hendy, CN Bernstein, JE Ghia
Biomedicines, 2021;9(2):. 2021
SOX9 Knockout Induces Polyploidy and Changes Sensitivity to Tumor Treatment Strategies in a Chondrosarcoma Cell Line
Authors: S Stöckl, G Lindner, S Li, P Schuster, S Haferkamp, F Wagner, PM Prodinger, G Multhoff, M Boxberg, A Hillmann, RJ Bauer, S Grässel
Int J Mol Sci, 2020;21(20):. 2020
Synergisms of genome and metabolism stabilizing antitumor therapy (GMSAT) in human breast and colon cancer cell lines: a novel approach to screen for synergism
Authors: J Ruhnau, J Parczyk, K Danker, B Eickholt, A Klein
BMC Cancer, 2020;20(1):617. 2020
Targeting the ABC transporter ABCB5 sensitizes glioblastoma to temozolomide-induced apoptosis through a cell-cycle checkpoint regulation mechanism
Authors: CAA Lee, P Banerjee, BJ Wilson, S Wu, Q Guo, G Berg, S Karpova, A Mishra, JW Lian, J Tran, M Emmerich, GF Murphy, MH Frank, NY Frank
J. Biol. Chem., 2020;0(0):. 2020
l-tetrahydropalmatine suppresses osteoclastogenesis in vivo and in vitro via blocking RANK-TRAF6 interactions and inhibiting NF-kappaB and MAPK pathways
Authors: X Zhi, L Wang, H Chen, C Fang, J Cui, Y Hu, L Cao, W Weng, Q Zhou, L Qin, H Song, Y Wang, Y Wang, H Jiang, X Li, S Wang, X Chen, J Su
J. Cell. Mol. Med., 2019;0(0):. 2019
Inhibition of hyaluronic acid formation sensitizes chronic myelogenous leukemia to treatment with doxorubicin
Authors: Olga N Uchakina
Glycobiology, 2016;0(0):. 2016
ALDH1A3, the major aldehyde dehydrogenase isoform in human cholangiocarcinoma cells, affects prognosis and gemcitabine resistance in cholangiocarcinoma patients
Authors: MH Chen, JJ Weng, CT Cheng, RC Wu, SC Huang, CE Wu, YH Chung, CY Liu, MH Chang, MH Chen, KC Chiang, TS Yeh, Y Su, CN Yeh
Clin Cancer Res, 2016;0(0):. 2016
Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
Authors: Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA
Blood, 2007;109(4):1479-89. 2007
Suppression of Mycobacterium tuberculosis induced reactive oxygen species (ROS) and TNF-alpha mRNA expression in human monocytes by allicin.
Authors: Hasan N, Yusuf N, Toossi Z, Islam N
FEBS Lett., 2006;580(10):2517-22. 2006
A functional screening of the kinome identifies the Polo-like kinase 4 as a potential therapeutic target for malignant rhabdoid tumors, and possibly, other embryonal tumors of the brain.
Authors: Sredni S, Suzuki M, Yang J, Topczewski J, Bailey A, Gokirmak T, Gross J, de Andrade A, Kondo A, Piper D, Tomita T
Pediatr Blood Cancer, 0;64(11):. 0
Characterization of CM572, a Selective Irreversible Partial Agonist of the Sigma-2 Receptor with Antitumor Activity.
Authors: Nicholson H, Comeau A, Mesangeau C, McCurdy C, Bowen W
J Pharmacol Exp Ther, 0;354(2):203-12. 0
In vitro antimicrobial studies of silver carbene complexes: activity of free and nanoparticle carbene formulations against clinical isolates of pathogenic bacteria.
Authors: Leid J, Ditto A, Knapp A, Shah P, Wright B, Blust R, Christensen L, Clemons C, Wilber J, Young G, Kang A, Panzner M, Cannon C, Yun Y, Youngs W, Seckinger N, Cope E
J Antimicrob Chemother, 0;67(1):138-48. 0
Identification of Chemical Compounds That Inhibit Protein Synthesis in Pseudomonas aeruginosa.
Authors: Palmer S, Hu Y, Keniry M, Bullard J
SLAS Discov, 0;22(6):775-782. 0
Comparison of electrospun and solvent cast polylactic acid (PLA)/poly(vinyl alcohol) (PVA) inserts as potential ocular drug delivery vehicles.
Authors: Bhattarai R, Das A, Alzhrani R, Kang D, Bhaduri S, Boddu S
Mater Sci Eng C Mater Biol Appl, 0;77(0):895-903. 0
Lenalidomide interferes with tumor-promoting properties of nurse-like cells in chronic lymphocytic leukemia.
Authors: Fiorcari S, Martinelli S, Bulgarelli J, Audrito V, Zucchini P, Colaci E, Potenza L, Narni F, Luppi M, Deaglio S, Marasca R, Maffei R
Haematologica, 0;100(2):253-62. 0
Integrative genomics identified RFC3 as an amplified candidate oncogene in esophageal adenocarcinoma.
Authors: Lockwood W, Thu K, Lin L, Pikor L, Chari R, Lam W, Beer D
Clin Cancer Res, 0;18(7):1936-46. 0
Can mesenchymal stem cells pretreated with platelet-rich plasma modulate tissue remodeling in a rat with burned skin?
Authors: Hosni Ahmed H, Rashed L, Mahfouz S, Elsayed Hussein R, Alkaffas M, Mostafa S, Abusree A
Biochem Cell Biol, 0;95(5):537-548. 0
Imidazolium salts as small-molecule urinary bladder exfoliants in a murine model.
Authors: Wagers P, Tiemann K, Shelton K, Kofron W, Panzner M, Wooley K, Youngs W, Hunstad D
Antimicrob Agents Chemother, 0;59(9):5494-502. 0
Development and qualification of an LC-MS/MS method for investigating the biological implications of micelle entrapped paclitaxel in cell culture and rats.
Authors: Kaddoumi A, Gill K, Elfakhri K, Nazzal S
Biomed Chromatogr, 0;31(9):. 0
Anticancer and Anti-Inflammatory Properties of Ganoderma lucidum Extract Effects on Melanoma and Triple-Negative Breast Cancer Treatment.
Authors: Barbieri A, Quagliariello V, Del Vecchio V, Falco M, Luciano A, Amruthraj N, Nasti G, Ottaiano A, Berretta M, Iaffaioli R, Arra C
Nutrients, 0;9(3):. 0
An easy method for bacterial expression and purification of wild-type and mutant superoxide dismutase 1 (SOD1).
Authors: Tachu B, Wusten K, Garza M, Wille H, Tamguney G
Protein Expr Purif, 0;134(0):63-71. 0
Vaccine-induced tumor necrosis factor-producing T cells synergize with cisplatin to promote tumor cell death.
Authors: van der Sluis T, van Duikeren S, Huppelschoten S, Jordanova E, Beyranvand Nejad E, Sloots A, Boon L, Smit V, Welters M, Ossendorp F, van de Water B, Arens R, van der Burg S, Melief C
Clin Cancer Res, 0;21(4):781-94. 0
Identification of Chemical Compounds That Inhibit the Function of Glutamyl-tRNA Synthetase from Pseudomonas aeruginosa.
Authors: Hu Y, Guerrero E, Keniry M, Manrrique J, Bullard J
J Biomol Screen, 0;20(9):1160-70. 0
Synergistic effect of Ebselen and gamma radiation on breast cancer cells.
Authors: Thabet N, Moustafa E
Int J Radiat Biol, 0;93(8):784-792. 0
Identification of a negative regulatory role for spi-C in the murine B cell lineage.
Authors: Li S, Solomon L, Fulkerson P, DeKoter R
J Immunol, 0;194(8):3798-807. 0
Regulation of bovine pyruvate carboxylase mRNA and promoter expression by thermal stress.
Authors: White H, Koser S, Donkin S
J Anim Sci, 0;90(9):2979-87. 0
A Gene Expression Signature Associated with Overall Survival in Patients with Hepatocellular Carcinoma Suggests a New Treatment Strategy.
Authors: Gillet J, Andersen J, Madigan J, Varma S, Bagni R, Powell K, Burgan W, Wu C, Calcagno A, Ambudkar S, Thorgeirsson S, Gottesman M
Mol Pharmacol, 0;89(2):263-72. 0
Nicotine Reduces Survival via Augmentation of Paracrine HGF-MET Signaling in the Pancreatic Cancer Microenvironment.
Authors: Delitto D, Zhang D, Han S, Black B, Knowlton A, Vlada A, Sarosi G, Behrns K, Thomas R, Lu X, Liu C, George T, Hughes S, Wallet S, Trevino J
Clin Cancer Res, 0;22(7):1787-99. 0
In vitro cytotoxicity assessment of a West Virginia chemical spill mixture involving 4-methylcyclohexanemethanol and propylene glycol phenyl ether.
Authors: Han A, Fabyanic E, Miller J, Prediger M, Prince N, Mouch J, Boyd J
Environ Monit Assess, 0;189(4):190. 0
Prothymosin-alpha interacts with mutant huntingtin and suppresses its cytotoxicity in cell culture.
Authors: Dong G, Callegari E, Gloeckner C, Ueffing M, Wang H
J Biol Chem, 0;287(2):1279-89. 0
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