Cultrex RGF Basement Membrane Extract, Type 2
Cultrex RGF Basement Membrane Extract, Type 2 Summary
Gelling Assay - BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.
Murine Engelbreth-Holm-Swarm (EHS) tumor.
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||12 - 18 mg/mL.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Product is stable for a minimum of 3 months from date of shipment when stored at –20°C in a manual defrost freezer. For optimal stability, store at –80°C. Avoid freeze-thaw cycles.
Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 μg/ml gentamicin sulfate.
• PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.
• No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
Background: Basement Membrane Extracts
Basement Membrane Extract (BME) is a soluble form of basement membrane
purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at
37°C to form a reconstituted basement membrane. The major components of
BME include laminin, collagen IV, entactin, and heparin sulfate
proteoglycan. BME can be used in a multiple applications, under a
variety of cell culture conditions, for maintaining growth or promoting
differentiation of primary endothelial, epithelial, smooth muscle and
stem cells. BME can also be utilized in cell attachment, neurite
outgrowth, angiogenesis, in vitro cell invasion and in vivo
Recently we have developed two additional formulations of Cultrex® BME known as Cultrex® BME Type 2 and Cultrex® BME Type 3. Cultrex® BME Type 2 provides a proprietary formulation that is higher in tensile strength when compared to our original BME, while Cultrex® BME Type 3 is physiologically aligned with the in vivo solid tumors environment and is recommended for xenografts and other in vivo applications.
There are many applications for Cultrex® BME, which require different thicknesses and concentrations. In general, BME at a protein concentration ≥ 10 mg/ml is used for differentiation studies of primary cells. For applications such as endothelial cell formation of capillary-like structures (Tube Formation Assay), the differentiation of rat aorta tissue into capillary-like structures (Aortic Ring Assay), epithelial organoid formation, or tumor organoid formation, a thick gel is needed. Some applications, such as propagation of primary cells, require a thin layer coating and not a thick gel; therefore, the thin layer method should be used.
Citation for Cultrex RGF Basement Membrane Extract, Type 2
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Crosslinked basement membrane-based coatings enhance glucose sensor function and continuous glucose monitoring in vivo.
Authors: Klueh U, Ludzinska I, Czajkowski C, Qiao Y, Kreutzer D
J Biomed Mater Res A, 0;106(1):7-16.
Sample Types: In Vivo
What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
How should Cultrex Basement Membrane Extract (BME) be stored and handled?
Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.
Animal-Free Recombinant Proteins
GMP Recombinant Proteins
Tocris Small Molecules
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