Home / Basement Membrane Extracts / Cultrex RGF Basement Membrane Extract, Type 2

Cultrex RGF Basement Membrane Extract, Type 2

Catalog #: 3533-005-02
33 Citations Datasheet / COA / SDS
For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract
Catalog # Availability Size / Price Qty
3533-005-02
Product Details
Citations (33)
FAQs
Reviews
Summary Product Datasheets Background

Cultrex RGF Basement Membrane Extract, Type 2 Summary

Specifications

Source
N/A
Endotoxin Level
<8.0 EU per 1 μg of the protein by the LAL method.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Product is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles.

Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 μg/ml gentamicin sulfate.

Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines.
Applications
Bioactivity
Species
Mouse

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Cultrex RGF Basement Membrane Extract, Type 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. GABAergic signaling contributes to tumor cell invasion and poor overall survival in colorectal cancer
    Authors: Strelez, C;Battaglin, F;Perez, R;Yang, Y;Cherry, C;Millstein, J;Yoon, AY;Chlystek, JS;Canfield, E;Haliday, B;Shah, C;Ghaffarian, K;Soni, S;Jiang, H;Lau, R;Schatz, A;Zhou, Y;Mulkerin, D;Ou, FS;Venook, AP;Innocenti, F;Neman, J;Katz, JE;Lenz, HJ;Mumenthaler, SM;
    Oncogene  2025-08-24
  2. Generation of human fetal brain organoids and their CRISPR engineering for brain tumor modeling
    Authors: Pagliaro, A;Andreatta, F;Finger, R;Artegiani, B;Hendriks, D;
    Nature protocols  2025-02-06
  3. miR-449a/miR-340 reprogram cell identity and metabolism in fusion-negative rhabdomyosarcoma
    Authors: Pozzo, E;Yedigaryan, L;Giarratana, N;Wang, CC;Garrido, GM;Degreef, E;Marini, V;Rinaldi, G;van der Veer, BK;Sassi, G;Eelen, G;Planque, M;Fanzani, A;Koh, KP;Carmeliet, P;Yustein, JT;Fendt, SM;Uyttebroeck, A;Sampaolesi, M;
    Cell reports  2025-01-11
  4. A developmental biliary lineage program cooperates with Wnt activation to promote cell proliferation in hepatoblastoma
    Authors: Wu, PV;Fish, M;Hazard, FK;Zhu, C;Vennam, S;Walton, H;Wagh, D;Coller, J;Przybyl, J;Morri, M;Neff, N;West, RB;Nusse, R;
    Nature communications  2024-11-20
  5. More powerful dysregulation of Helicobacter pylori East Asian-type CagA on intracellular signalings
    Authors: Ji, X;Sun, Z;Wu, H;Zhang, J;Liu, S;Cao, X;Wang, B;Wang, F;Zhang, Y;Li, B;Feng, J;Zhao, H;
    BMC microbiology  2024-11-11
  6. Mutant TP53 switches therapeutic vulnerability during gastric cancer progression within interleukin-6 family cytokines
    Authors: Huber, A;Allam, AH;Dijkstra, C;Thiem, S;Huynh, J;Poh, AR;Konecnik, J;Jacob, SP;Busuttil, R;Liao, Y;Chisanga, D;Shi, W;Alorro, MG;Forrow, S;Tauriello, DVF;Batlle, E;Boussioutas, A;Williams, DS;Buchert, M;Ernst, M;Eissmann, MF;
    Cell reports  2024-08-27
  7. Derivation of functional thymic epithelial organoid lines from adult murine thymus
    Authors: Lim, S;J F van Son, G;Wisma Eka Yanti, NL;Andersson-Rolf, A;Willemsen, S;Korving, J;Lee, HG;Begthel, H;Clevers, H;
    Cell reports  2024-03-27
  8. BEHAV3D: a 3D live imaging platform for comprehensive analysis of engineered T cell behavior and tumor response
    Authors: Alieva, M;Barrera Román, M;de Blank, S;Petcu, D;Zeeman, AL;Dautzenberg, NMM;Cornel, AM;van de Ven, C;Pieters, R;den Boer, ML;Nierkens, S;Calkoen, FGJ;Clevers, H;Kuball, J;Sebestyén, Z;Wehrens, EJ;Dekkers, JF;Rios, AC;
    Nature protocols  2024-03-19
  9. Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p
    Authors: Kang, SH;Oh, SY;Lee, KY;Lee, HJ;Kim, MS;Kwon, TG;Kim, JW;Lee, ST;Choi, SY;Hong, SH;
    Theranostics  2024-01-01
  10. Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
    Authors: Nzigou Mombo, B;Bijonowski, BM;Raab, CA;Niland, S;Brockhaus, K;Müller, M;Eble, JA;Wegner, SV;
    Nature communications  2023-10-09
  11. Integration of Patient-Derived Organoids and Organ-on-Chip Systems: Investigating Colorectal Cancer Invasion within the Mechanical and GABAergic Tumor Microenvironment
    Authors: Strelez, C;Perez, R;Chlystek, JS;Cherry, C;Yoon, AY;Haliday, B;Shah, C;Ghaffarian, K;Sun, RX;Jiang, H;Lau, R;Schatz, A;Lenz, HJ;Katz, JE;Mumenthaler, SM;
    bioRxiv : the preprint server for biology  2023-09-17
  12. TMEM123 a key player in immune surveillance of colorectal cancer
    Authors: Elisa Pesce, Chiara Cordiglieri, Mauro Bombaci, Serenella Eppenberger-Castori, Stefania Oliveto, Cristina Manara et al.
    Frontiers in Immunology  2023-06-23
  13. gammadelta T cells are effectors of immunotherapy in cancers with HLA class I defects
    Authors: NL de Vries, J van de Haa, V Veninga, M Chalabi, ME Ijsselstei, M van der Pl, J van den Bu, D Ruano, JG van den Be, JB Haanen, LJ Zeverijn, BS Geurts, GF de Wit, TW Battaglia, H Gelderblom, HMW Verheul, TN Schumacher, LFA Wessels, F Koning, NFCC de Miranda, EE Voest
    Nature, 2023-01-11;613(7945):743-750.  2023-01-11
  14. A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing
    Authors: M Vizoso, C E J Pritch, L Bombardell, B van den Br, P Krimpenfor, RL Beijersber, K Jalink, J van Rheene
    Nature Communications, 2022-10-28;13(1):6442.  2022-10-28
  15. Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells
    Authors: L Adnani, J Kassouf, B Meehan, C Spinelli, N Tawil, I Nakano, J Rak
    Nature Communications, 2022-09-19;13(1):5494.  2022-09-19
  16. Mesenchymal tumor organoid models recapitulate rhabdomyosarcoma subtypes
    Authors: MT Meister, MJA Groot Koer, T de Souza, WB Breunis, E Frazer-Men, M Brok, J DeMartino, F Manders, C Calandrini, HHD Kerstens, A Janse, MEM Dolman, S Eising, KPS Langenberg, M van Tuil, RRG Knops, ST van Schelt, LS Hiemcke-Ji, U Flucke, JHM Merks, MM van Noesel, BBJ Tops, JY Hehir-Kwa, P Kemmeren, JJ Molenaar, M van de Wet, R van Boxtel, J Drost, FCP Holstege
    Embo Molecular Medicine, 2022-08-02;0(0):e16001.  2022-08-02
  17. WNK1 collaborates with TGF-beta in endothelial cell junction turnover and angiogenesis
    Authors: AB Jaykumar, S Plumber, DM Barry, D Binns, C Wichaidit, M Grzemska, S Earnest, EJ Goldsmith, O Cleaver, MH Cobb
    Proceedings of the National Academy of Sciences of the United States of America, 2022-07-22;119(30):e2203743119.  2022-07-22
  18. Epithelial-to-Mesenchymal Transition Drives Invasiveness of Breast Cancer Brain Metastases
    Authors: AS Margarido, R Uceda-Cast, K Hahn, R de Bruijn, L Kester, I Hofland, J Lohuis, D Seinstra, A Broeks, J Jonkers, MLD Broekman, P Wesseling, C Vennin, M Vizoso, J van Rheene
    Cancers, 2022-06-25;14(13):.  2022-06-25
  19. Sorafenib and nitazoxanide disrupt mitochondrial function and inhibit regrowth capacity in three-dimensional models of hepatocellular and colorectal carcinoma
    Authors: F Ek, K Blom, T Selvin, J Rudfeldt, C Andersson, W Senkowski, C Brechot, P Nygren, R Larsson, M Jarvius, M Fryknäs
    Scientific Reports, 2022-05-27;12(1):8943.  2022-05-27
  20. FER regulates endosomal recycling and is a predictor for adjuvant taxane benefit in breast cancer
    Authors: S Tavares, N Liv, M Pasolli, M Opdam, MAK Rätze, M Saornil, LM Sluimer, RCC Hengeveld, R van Es, E van Werkho, H Vos, H Rehmann, BMT Burgering, HM Oosterkamp, SMA Lens, J Klumperman, SC Linn, PWB Derksen
    Cell Reports, 2022-04-05;39(1):110584.  2022-04-05
  21. Early invasion of the bladder wall by solitary bacteria protects UPEC from antibiotics and neutrophil swarms in an organoid model
    Authors: K Sharma, VV Thacker, N Dhar, M Clapés Cab, A Dubois, F Signorino-, J Mullenders, GW Knott, H Clevers, JD McKinney
    Cell Reports, 2021-07-20;36(3):109351.  2021-07-20
  22. Active elimination of intestinal cells drives oncogenic growth in organoids
    Authors: A Krotenberg, A Fumagalli, HQ Le, R Jackstadt, TRM Lannagan, OJ Sansom, J van Rheene, SJE Suijkerbui
    Cell Reports, 2021-07-06;36(1):109307.  2021-07-06
  23. Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer
    Authors: K Lõhmussaar, R Oka, J Espejo Val, MHH Smits, H Wardak, J Korving, H Begthel, N Proost, M van de Ven, OW Kranenburg, TGN Jonges, RP Zweemer, S Veersema, R van Boxtel, H Clevers
    Cell Stem Cell, 2021-04-13;0(0):.  2021-04-13
  24. Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids
    Authors: JF Dekkers, EJ van Vliet, N Sachs, JM Rosenbluth, O Kopper, HG Rebel, EJ Wehrens, C Piani, JE Visvader, CS Verissimo, SF Boj, JS Brugge, H Clevers, AC Rios
    Nature Protocols, 2021-03-10;0(0):.  2021-03-10
  25. SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site
    Authors: AZ Mykytyn, TI Breugem, S Riesebosch, D Schipper, PB van den Do, RJ Rottier, MM Lamers, BL Haagmans
    Elife, 2021-01-04;10(0):.  2021-01-04
  26. Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver
    Authors: D Hendriks, B Artegiani, H Hu, S Chuva de S, H Clevers
    Nature Protocols, 2020-11-27;0(0):.  2020-11-27
  27. Establishment of patient-derived cancer organoids for drug-screening applications
    Authors: E Driehuis, K Kretzschma, H Clevers
    Nat Protoc, 2020-09-14;15(10):3380-3409.  2020-09-14
  28. Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer
    Authors: A Fumagalli, KC Oost, L Kester, J Morgner, L Bornes, L Bruens, L Spaargaren, M Azkanaz, T Schelfhors, E Beerling, MC Heinz, D Postrach, D Seinstra, AM Sieuwerts, JWM Martens, S van der El, M van Baalen, D Bhowmick, N Vrisekoop, SIJ Ellenbroek, SJE Suijkerbui, HJ Snippert, J van Rheene
    Cell Stem Cell, 2020-03-12;0(0):.  2020-03-12
  29. EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
    Authors: F Gimeno-Val, ÁL Riffo-Camp, G Ayala, N Tarazona, V Gambardell, FM Rodríguez, M Huerta, C Martínez-C, J Montón-Bue, S Roselló, D Roda, A Cervantes, L Franco, G López-Roda, J Castillo
    Sci Rep, 2020-02-28;10(1):3723.  2020-02-28
  30. Characterization of organoid cultured human breast cancer
    Authors: N Goldhammer, J Kim, V Timmermans, OW Petersen
    Breast Cancer Res., 2019-12-11;21(1):141.  2019-12-11
  31. Synergistic cancer immunotherapy combines MVA-CD40L induced innate and adaptive immunity with tumor targeting antibodies
    Authors: J Medina-Ech, M Hinterberg, M Testori, M Geiger, R Giessel, B Bathke, R Kassub, F Gräbnitz, G Fiore, ST Wennier, P Chaplin, M Suter, H Hochrein, H Lauterbach
    Nat Commun, 2019-11-06;10(1):5041.  2019-11-06
  32. Patient-Derived Head and Neck Cancer Organoids Recapitulate EGFR Expression Levels of Respective Tissues and Are Responsive to EGFR-Targeted Photodynamic Therapy
    Authors: E Driehuis, S Spelier, I Beltrán He, R de Bree, S M Willems, H Clevers, S Oliveira
    J Clin Med, 2019-11-05;8(11):.  2019-11-05
  33. Crosslinked basement membrane-based coatings enhance glucose sensor function and continuous glucose monitoring in vivo.
    Authors: Klueh U, Ludzinska I, Czajkowski C, Qiao Y, Kreutzer D
    J Biomed Mater Res A, 2017-09-19;106(1):7-16.  2017-09-19

FAQs

  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

  3. How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  4. Does Cultrex BME, Catalog # 3533-005-02, affect fluorescence readings when Alamar Blue is used for final readout of assay? 

    • BME is known to have autofluorescence, but if appropriate controls are evaluated, background can be successfully subtracted. A BME only control well with no cells should be used to subtract the background fluorescence.

View all Cell Culture Product FAQs

Reviews for Cultrex RGF Basement Membrane Extract, Type 2

There are currently no reviews for this product. Be the first to review Cultrex RGF Basement Membrane Extract, Type 2 and earn rewards!

Have you used Cultrex RGF Basement Membrane Extract, Type 2?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review