Tenascin C Antibody (4C8MS) - BSA Free

Novus Biologicals | Catalog # NB110-68136

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Feline

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4C8MS

Format

BSA Free
View all Tenascin C Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Tenascin C [Swiss-Prot# P24821].

Reactivity Notes

Feline reactivity reported in customer review.

Localization

Secreted > extracellular space > extracellular matrix

Specificity

NB110-68136 specifically reacts with Domain B on FNIII repeats of Tenascin C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Tenascin C Antibody (4C8MS) - BSA Free

Flow (Intracellular): Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow (Intracellular): Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].
Western Blot: Tenascin C Antibody (4C8MS)BSA Free [NB110-68136]

Western Blot: Tenascin C Antibody (4C8MS)BSA Free [NB110-68136]

Tenascin-C-Antibody-4C8MS-Western-Blot-NB110-68136-img0015.jpg
Immunocytochemistry/ Immunofluorescence: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunocytochemistry/ Immunofluorescence: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.
Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136] - An intracellular stain was performed on MCF7 cells with Tenascin C Antibody (4C8MS) NB110-68136 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).
Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.
Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody [4C8MS] NB110-68136AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for Tenascin C Antibody (4C8MS) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

5 ug/ml
Application Notes
For use in IHC-P, it is recommended to incubate primary antibody for at least 2 hours at room temperature followed by ON incubation at 4C. This antibody is CyTOF ready.

Reviewed Applications

Read 2 reviews rated 3.5 using NB110-68136 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tenascin C

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).

Alternate Names

Cytotactin, HXB, Tenascin J1, TNC

Entrez Gene IDs

3371 (Human); 116640 (Rat)

Gene Symbol

TNC

UniProt

P24821 (Human)

Additional Tenascin C Products

Product Documents for Tenascin C Antibody (4C8MS) - BSA Free

Certificate of Analysis

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Product Specific Notices for Tenascin C Antibody (4C8MS) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Tenascin C Antibody (4C8MS) - BSA Free

Customer Reviews for Tenascin C Antibody (4C8MS) - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Tenascin C Antibody (4C8MS)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Sclera
    Species: Feline
    Verified Customer | Posted 09/15/2018
    Tenascin-C antibody was immunolabeled shown as red. DAPI (blue). The image was captured using epifluorescent microscopy.
    Tenascin C Antibody (4C8MS) - BSA Free NB110-68136
  • Tenascin C Antibody (4C8MS)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Uterus tissue
    Species: Human
    Verified Customer | Posted 03/22/2017
    Tenascin C Antibody (4C8MS) - BSA Free NB110-68136

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Protocols

View specific protocols for Tenascin C Antibody (4C8MS) - BSA Free (NB110-68136):


Immunohistochemistry-Paraffin Embedded Sections for NB110-68136

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Tenascin C Antibody (4C8MS) - BSA Free

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  • Q: I would like to see image of WB to see the isoforms of the tenascin NB110-68136.

    A:

    I am afraid we do not have notes or images on the westerns for this antibody. There are 2 publications that do have a double band in westerns for this antibody: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680762/. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868127/. We have 2 Tenascin C antibodies that have been validated in mouse for western blot but none with images.

  • Q: One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?

    A:

    Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P

  • Q: I would like to see image of WB to see the isoforms of the tenascin NB110-68136.

    A:

    I am afraid we do not have notes or images on the westerns for this antibody. There are 2 publications that do have a double band in westerns for this antibody: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680762/. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868127/. We have 2 Tenascin C antibodies that have been validated in mouse for western blot but none with images.

  • Q: One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?

    A:

    Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P

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