Mouse TGF-beta 1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-92671

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate), buffered solution (10 - 40 uL (pretreated))

Sensitivity

9.0 pg/mL

Assay Range

31.3 - 2,000 pg/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

The assay detects both natural and recombinant mouse TGF-beta1. The cross reactivity of TGF-beta2 and TGF-beta3, and of TNF-beta, IL-8, IL-6, IL- 2, TNF-alpha, IL-1beta, IL-4, IFN-gamma, IL12p70, IL-5 and IL-10 was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no cross reactivity detected.

Description

The mouse TGF-beta1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse TGF-beta1.

Precision

Intra-Assay Precision (Precision within an assay) CV% 6.8%

Inter-Assay Precision (Precision between assays) CV% 6.8%

Recovery for Mouse TGF-beta 1 ELISA Kit (Colorimetric)

Recovery

Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Mouse TGF-beta 1 ELISA Kit (Colorimetric)

ELISA: Mouse TGF-beta 1 ELISA Kit [NBP1-92671]

Kit Contents for Mouse TGF-beta 1 ELISA Kit (Colorimetric)

  • ]Substrate Solution (tetramethyl-benzidine)
  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse TGF-beta1
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse TGF-beta1 polyclonal antibody
  • Mouse TGF-beta1 Standard lyophilized, 4 ng/mL upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TGF-beta 1

TGF-beta plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. It has both tumor suppressive and tumor promoting activities and is highly regulated at all levels. TGF-beta1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF-beta1 promotes Treg cell differentiation resulting in IL-10/TGF-beta1 production and Th1 cell and Th2 cell suppression.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for Mouse TGF-beta 1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse TGF-beta 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Mouse TGF-beta 1 ELISA Kit (Colorimetric)

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FAQs for Mouse TGF-beta 1 ELISA Kit (Colorimetric)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

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