Canine TGF-beta 1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60147

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

0.747 ng/mL

Assay Range

0.781 ng/mL - 50 ng/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Canine

Specificity

This assay has high sensitivity and excellent specificity for detection of dog TGF-B1. No significant cross-reactivity or interference between dog TGF-B1 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Canine TGF-beta 1 ELISA Kit (Colorimetric)

ELISA: Canine TGF-beta 1 ELISA Kit (Colorimetric) [NBP2-60147]

ELISA: Canine TGF-beta 1 ELISA Kit (Colorimetric) [NBP2-60147]

ELISA: Canine TGF-beta 1 ELISA Kit (Colorimetric) [NBP2-60147] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Canine TGF-beta 1 ELISA Kit (Colorimetric)

Canine TGF-beta 1 ELISA Kit (Colorimetric) [NBP2-60147] -

Canine TGF-beta 1 ELISA Kit (Colorimetric) [NBP2-60147] - Standard Curve Reference

Kit Contents for Canine TGF-beta 1 ELISA Kit (Colorimetric)

  • 1 N HCI
  • 1.2 N NaOH/0.5 M HEPES
  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TGF-beta 1

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGF-B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-B1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for Canine TGF-beta 1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Canine TGF-beta 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Canine TGF-beta 1 ELISA Kit (Colorimetric)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

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