Recombinant Mouse TGF-beta 1 His Protein

Novus Biologicals | Catalog # NBP2-51925

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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all TGF-beta 1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 279-390 of Mouse TGF-beta 1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSALDTNYC FSSTEKNCCV RQLYIDFRKD LGWKWIHEPK GYHANFCLGP CPYIWSLDTQ YSKVLALYNQ HNPGASASPC CVPQALEPLP IVYYVGRKPK VEQLSNMIVR SCKCS

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

15.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse TGF-beta 1 His Protein

SDS-PAGE: Recombinant Mouse TGF-beta 1 His Protein [NBP2-51925]

SDS-PAGE: Recombinant Mouse TGF-beta 1 His Protein [NBP2-51925]

SDS-Page: Recombinant Mouse TGF-beta 1 Protein [NBP2-51925]

Formulation, Preparation, and Storage

NBP2-51925
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TGF-beta 1

TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule - the amino acid sequence between human and mouse differ only by one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for Recombinant Mouse TGF-beta 1 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse TGF-beta 1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Mouse TGF-beta 1 His Protein

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FAQs for Recombinant Mouse TGF-beta 1 His Protein

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

  • Q: Does TGF-beta 1 react across multiple species?

    A: TGF-beta 1 is a highly conserved molecule varying by just a few amino acids  between most species, and thus it is extremely likely to react across species. For example, human TGF-beta 1 is active on mouse cells and vice versa.

  • Q: Will Recombinant Human TGF-beta 1 support the maintenance of either human or non-human cultured cells?

    A: TGF-beta 1 performs multiple cellular functions, including cell growth, proliferation, differentiation and apoptosis.  It would be necessary to determine the specific conditions for cell growth/proliferation for the cells being cultured.

Showing  1 - 2 of 2 FAQs Showing All
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