TLE3 Antibody (CL3573) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43936

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3573

Format

Azide and BSA Free
View all TLE3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q04726, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TLE3 Antibody (CL3573) - Azide and BSA Free

TLE3 Antibody (CL3573) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TLE3 Antibody [NBP3-43936]

Immunocytochemistry/ Immunofluorescence: TLE3 Antibody [NBP3-43936]

Staining of HeLa cells using the Anti-TLE3 monoclonal antibody).
TLE3 Antibody (CL3573) - Azide and BSA Free Western Blot: TLE3 Antibody [NBP3-43936]

Western Blot: TLE3 Antibody [NBP3-43936]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line HEK 293
TLE3 Antibody (CL3573) - Azide and BSA Free Western Blot: TLE3 Antibody [NBP3-43936]

Western Blot: TLE3 Antibody [NBP3-43936]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line HeLa nuclear extract
TLE3 Antibody (CL3573) - Azide and BSA Free Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Staining of human kidney shows weak to moderate nuclear positivity in a subset of cells.
TLE3 Antibody (CL3573) - Azide and BSA Free Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Staining of human cervix shows strong nuclear immunoreactivity in epithelial cells.
TLE3 Antibody (CL3573) - Azide and BSA Free Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Staining of human oral mucosa shows strong nuclear positivity in epithelial cells.
TLE3 Antibody (CL3573) - Azide and BSA Free Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
TLE3 Antibody (CL3573) - Azide and BSA Free Western Blot: TLE3 Antibody [NBP3-43936]

Western Blot: TLE3 Antibody [NBP3-43936]

Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TLE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
TLE3 Antibody (CL3573) - Azide and BSA Free Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Staining of human small intestine shows moderate nuclear immunoreactivity in glandular epithelium cells.
TLE3 Antibody (CL3573) - Azide and BSA Free Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP3-43936]

Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells.

Applications for TLE3 Antibody (CL3573) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLE3

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES

Long Name

Transducin-like Enhancer of Split 3

Alternate Names

ESG3, Grg3

Gene Symbol

TLE3

Additional TLE3 Products

Product Documents for TLE3 Antibody (CL3573) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TLE3 Antibody (CL3573) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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