TLE3 Antibody (CL3573) - BSA Free

Novus Biologicals | Catalog # NBP2-61621

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3573

Format

BSA Free
View all TLE3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q04726, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TLE3 Antibody (CL3573) - BSA Free

Western Blot: TLE3 Antibody (CL3573) [NBP2-61621]

Western Blot: TLE3 Antibody (CL3573) [NBP2-61621]

Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TLE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: TLE3 Antibody (CL3573) [NBP2-61621]

Western Blot: TLE3 Antibody (CL3573) [NBP2-61621]

Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line HEK 293
Immunocytochemistry/ Immunofluorescence: TLE3 Antibody (CL3573) [NBP2-61621]

Immunocytochemistry/ Immunofluorescence: TLE3 Antibody (CL3573) [NBP2-61621]

Immunocytochemistry/Immunofluorescence: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of HeLa cells using the Anti-TLE3 monoclonal antibody, showing specific staining in the nucleoplasm and nucleoi in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
Western Blot: TLE3 Antibody (CL3573) [NBP2-61621]

Western Blot: TLE3 Antibody (CL3573) [NBP2-61621]

Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line HeLa nuclear extract
Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human cervix shows strong nuclear immunoreactivity in epithelial cells.
Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human oral mucosa shows strong nuclear positivity in epithelial cells.
Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human small intestine shows moderate nuclear immunoreactivity in glandular epithelium cells.
Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621]

Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human kidney shows weak to moderate nuclear positivity in a subset of cells.

Applications for TLE3 Antibody (CL3573) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:10000 - 1:20000

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLE3

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES

Long Name

Transducin-like Enhancer of Split 3

Alternate Names

ESG3, Grg3

Gene Symbol

TLE3

Additional TLE3 Products

Product Documents for TLE3 Antibody (CL3573) - BSA Free

Certificate of Analysis

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Product Specific Notices for TLE3 Antibody (CL3573) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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