TLR2 Antibody (TL2.1) - BSA Free

Novus Biologicals | Catalog # NB100-56722

Clone TL2.1 was used by HLDA to establish CD designation.
Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Rabbit

Cited:

Human, Mouse, Rat, Canine, Rabbit

Applications

Validated:

Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot, Functional, In vitro assay

Cited:

Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Cytometric Bead Assay Standard, In vitro assay, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # TL2.1

Format

BSA Free
View all TLR2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (PMID: 10657659). The hybridoma supernatants were selected by flow cytometry.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28219705, 29501923). Rat reactivity reported in scientific literature (PMID: 29501923).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TLR2 Antibody (TL2.1) - BSA Free

Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722]

Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722]

Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.
Western Blot: TLR2 Antibody (TL2.1)BSA Free [NB100-56722]

Western Blot: TLR2 Antibody (TL2.1)BSA Free [NB100-56722]

TLR2-Antibody-TL2-1-Western-Blot-NB100-56722-img0015.jpg
Western Blot: TLR2 Antibody (TL2.1)BSA Free [NB100-56722]

Western Blot: TLR2 Antibody (TL2.1)BSA Free [NB100-56722]

Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Total protein from human THP-1 and U937 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TLR2 (NB100-56722) in 5% BSA-TBST and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).
Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722]

Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722]

TLR2-Antibody-TL2-1-Flow-Cytometry-NB100-56722-img0013.jpg
Flow (Cell Surface): TLR2 Antibody (TL2.1) - BSA Free [NB100-56722]

Flow (Cell Surface): TLR2 Antibody (TL2.1) - BSA Free [NB100-56722]

Flow (Cell Surface): TLR2 Antibody (TL2.1) [NB100-56722] - Flow (Cell Surface): - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on stable transfected cell line using this antibody at 1 ug/10^6 cells. Green: isotype control. Red: anti-TLR2 antibody. Image using the Azide Free form of this antibody.
TLR2-Antibody-TL2-1-Block-Neutralize-NB100-56722-img0014.jpg
TLR2 Antibody (TL2.1) - BSA Free

Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722] -

Flow Cytometry: TLR2 Antibody (TL2.1) - BSA Free [NB100-56722] - Intracellular flow analysis of TLR2 in 1x10^6 PBMCs using 2 ug of NB100-56722. Shaded histogram represents cells without antibody; green represents isotype control; purple represents anti-TLR2 antibody.

Applications for TLR2 Antibody (TL2.1) - BSA Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 25412776)

Dot Blot

reported in scientific literature (PMID 27248820)

ELISA

reported in multiple pieces of scientific literature

Flow (Cell Surface)

reported in scientific literature (PMID 24836676)

Flow Cytometry

1 ug/10^6 cells

Functional

reported in scientific literature (PMID 25412776)

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

2-5 ug

In vitro assay

reported in scientific literature (PMID 24512642)

Western Blot

1 - 2 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR2

The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for TLR2 Antibody (TL2.1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TLR2 Antibody (TL2.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TLR2 Antibody (TL2.1) - BSA Free

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Protocols

View specific protocols for TLR2 Antibody (TL2.1) - BSA Free (NB100-56722):

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in 1.5% BSA and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructi

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